Central Role of Th2 and Tc2 Lymphocytes in Multiple Sclerosis Pattern II Demyelinating Lesions OmicsDI

ID: E-GEOD-60914

Date Released: 01-10-2016

Description: gical patterns of MS have been described. Pattern II is characterized by antibody and complement deposition. MS is considered a prototypic T cell-mediated disease, but until now the study of pathogenic T cells has encountered major challenges, most importantly the limited access of brain-infiltrating T cells. Here, we used next generation sequencing to identify clonally expand...

Mito-protective autophagy is impaired in erythroid cells of aged mtDNA-mutator mice OmicsDI

ID: E-GEOD-57761

Date Released: 04-27-2015

Description: ce. Genetic disruption of autophagy did not cause anemia in wild-type mice but accelerated the decline in mitochondrial respiration and development of macrocytic anemia in mtDNA-mutator mice. These findings highlight a pathological feedback loop that explains how dysfunctional mitochondria can escape autophagy-mediated degradation and propagate in cells predisposed to somatic mtDNA mutations, leading to disease. We used microarrays to identify expression profiles and pathways that are differentially activated or suppressed in Ter119+ bone marrow cells isolated from phlebotomized wildtype or Polg mutant mice...

Transcription profiling of human multiple myeloma patients classified using the TC system to provide a molecular characterization of the TC groups OmicsDI

ID: E-GEOD-2912

Date Released: 03-27-2012

Description: ntified 112 probe sets as characterizing the TC1, TC2, TC4 and TC5 groups, whereas the TC3 samples showed heterogeneous phenotypes and no marker genes. The TC2 group, which showed extra copies of the CCND1 locus and no IGH translocations or the chromosome 13q deletion, was characterized by the overexpression of genes involved in protein biosynthesis at translational level. A meta-analysis of published datasets validated the identified gene expression signatures. CONCLUSIONS. Our data contribute to the understanding of the molecular and biological features of distinct MM subtypes. The identification of a distinctive gene expression pattern in TC2 patients may improve risk stratification and indicate novel therapeutic targets. Experiment Overall Design: This series of microarray experiments contains the gene expression profiles of purified plasma cells (PCs) obtained from 50 newly diagnosed multiple myeloma (MM). PCs were purified from bone marrow specimens, after red blood cell lysis with 0.86% ammonium chloride, using CD138 immunomagnetic microbeads. The pu...

Proteomic analysis of integrin adhesion complex assembly OmicsDI

ID: GPM32310025299

Date Released:

Description: , PXD ID: PXD002159. Experiment: 20101108 JAA FN1 tc2 1-30, file: 20101108_JAA_FN_tc2_AB17.mzml. From ProteomeXchange: {{i}} To explore the dynamics of integrin adhesion complex assembly, isolated adhesion ...

Cag pathogenicity island-specific responses of gastric epithelial cells to Helicobacter pylori infection. OmicsDI

ID: E-GEOD-3556

Date Released: 10-18-2011

Description: 4) (the cagA 6 hour sample of trial 3 was lost). TC2: Biological replication and expansion of TC1, using more isogenic mutants and timepoints. AGS cells were mock infected, infected with G27, and isogenic mutants in cagN, cagA, cagE, and a deletion of the cag PAI for 0. 1, 3, 6, 12, and 24 hours. Probe synthesis and hybridization was done as in TC1. Note: there may have been a sample mix-up with PAI 12, swapping it with G27 or cagN 12. Groups of assays that are related as part of a time series. Computed...