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Displaying 20 of 213 results for "SMAD3"
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  1. GRP78 regulates ER homeostasis and distal epithelial cell survival during lung development BioProject

    ID: PRJNA287847

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: veolar epithelial cell (AEC) apoptosis. Increased Smad3 phosphorylation and expression of transforming growth factor-β (TGF-β)/Smad3 targets Cdkn1a (encoding p21) and Gadd45a suggest that interactions among the apoptotic arm of the UPR, oxidative stress and TGF-β/Smad signaling pathways contribute to Grp78 KO-induced AEC apoptosis and developmental arrest. Chemical chaperone taursodeoxycholic acid reduced UPR activation and apoptosis in cGrp78f/f lungs cultured ex vivo, confirming a role for ER stress in observed AEC abnormalities. These results demonstrate a key role for GRP78 in AEC survival and gene expression during lung development through modulation of ER stress and suggest the UPR as a potential therapeutic target in BPD. Overall design: Whole-genome expression profiling was performed using MouseRef-8 v2.0 Expression BeadChips (Illumina) on RNA isolated from lungs of four Grp78f/f and three cGrp78f/f mice at E18....
  2. DACH1 inhibits TGF-beta signaling through binding Smad4 BioProject

    ID: PRJNA87633

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ince the Ski/Sno proto-oncogenes repress AP-1 and SMAD signaling, we hypothesized that DACH1 might play a similar cellular function. Herein, DACH1 was found to be expressed in breast cancer cell lines and to inhibit TGF-beta induced apoptosis. DACH1 repressed TGF-beta induction of AP-1 and Smad signaling in gene reporter assays and repressed endogenous TGF-beta responsive genes by microarray analyses. DACH1 bound to endogenous NCoR and Smad4 in cultured cells and DACH1 co-localized with NCoR in nuclear dot-like...
  3. Expression profile of etoposide-resistant MCF7 (MCF7VP) cells with targeted RUNX2 knockdown ArrayExpress

    ID: E-GEOD-28414

    Description: tial expression of over 5000 genes (fold change > 2, P value < 0.05) indicate that several drug resistance mechanisms may be operating in these cells, including up-regulation of ABC transporter genes, down-regulation of the drug target and down-regulation of apoptotic genes. Several transcription factors such as RUNX2, SOX9, ETS1 and SMAD3 were up-regulated in the drug resistant cells. Targeted RUNX2 knockdown in the resistant cells using siRNA increased sensitivity to etoposide and also upregulated expression of pro-apoptotic genes indicating that RUNX2 could be a molecular target against etoposide resistance. Differential miRNA (microRNA) expression was observed among the drug resistant and sensitive cells suggesting that miRNA may also play a role...

  4. HNF4A-binding sites in HepG2 hepatoblastoma cells treated with TGF-beta BioProject

    ID: PRJNA143117

    Keywords: Epigenomics

    Access Type: download

    dataset.description: tion factors and cofactors that interact with the Smad complex. In the present study, we determined Smad2 and Smad3 (Smad2/3) binding regions in the promoters of known genes in HepG2 hepatoblastoma cells, and compared them to those in HaCaT epidermal keratinocytes to elucidate the m...
  5. Gene expression analysis of wild-type and Beta-2-spectrin homozygous knockout (β2SP-/-) mouse embryonic fibroblasts BioProject

    ID: PRJNA275639

    Keywords: Transcriptome or Gene expression

    Access Type: download

  6. Analysis of the Tgfb1/Smad3-regulated white adipose tissue (WAT) transcriptome in a mouse model of diet induced obesity NURSA

    Keywords: WAT epididymal

    Description: WAT was isolated from Smad3 KO and WT male mice fed a HFD (55% [w/w] fat content) or a control diet (5% [w/w] fat content) for 10 w. WAT was also isolate...

    ID: 10.1621/yG7RrbFzac

  7. Microarray Gene Expression for Undifferentiated Mesenchymal Stem Cells, Adipogenically Differentiated and Dedifferentiation cells BioProject

    ID: PRJNA157099

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: SESN3) and downregulation (DST, KAT2, MLL5, RB1, SMAD3, ZAK) of distinct genes play a curcial role in cell cycle to drive the adipogenically differentiated cells towards an arrested state to narrow down the lineage potency. However, the upregulation (CCND1, CHEK, HGF, HMGA2, SMAD3) and downregulation (CCPG1, RASSF4, RGS2) of these cell cycle genes motivates dedifferentiation of adipogenically differentiated cells to reverse the arrested state. We also found new fat markers along with signaling pathways for adipogenically differentiated and dedifferentiated cells, and also observed the influencing role of proliferation associated genes in cell cycle arrest and progression. We have differentiated bone marrow derived mesenchymal stem cells(MSC) into adipogenically differentiated cells followed by dedifferentiation. We are intrested to know new fat markers, signaling pathways for adipogenically differentiated and dedifferentiated cells, along to observe the genes associated with cell cycle arrest and progress...
  8. The TGF-b superfamily cytokine GDF15 is a CCN2-regulated autocrine/paracrine factor in the heart BioProject

    ID: PRJNA239002

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ing a concentration-dependent increase of phospho-Smad3(Ser423/425) (p<0.05, n=3), phospho-AKT(Ser473) (p<0.05, n=3) and phospho-IκBα(Ser32/36) (p<0.05, n=3) levels. However, rGDF15 did not phosphorylate Smad3(Ser423/425) or IκBα(Ser32/36) in cardiac myocyte. Cardiac fibroblasts exposed to rGDF15 for 48 hours displayed differentiation towards myofibroblasts reflected by increased levels of the differentiation marker α-smooth muscle actin (SMA) similar to cardiac fibroblasts stimulated with TGFβ. The effect of GDF15 on α-SMA was dose-dependent ranging from 500 nM - 20 nM rGDF15 (p<0.05, n=3). Differentiation towards a myofibroblast phenotype in the presence of GDF15 was also supported by higher matrix metalloproteinase (MMP) enzyme activity in the cell culture medium (6±1 fold increase, n=3, p<0.05) and increased e...
  9. Gallus gallus white isthmus transcriptome ArrayExpress

    ID: E-GEOD-52491

    Description: nforces the key role of the estrogen receptor and SMAD3 in mediating gene regulation during eggshell membrane synthesis. These results will assist with development of selection strategies to improve eggshell quality and food safety of the table egg. Keywords: Laying hen, eggshell, oviduct, Isthmus expression, cDNA microarray, indirect cDNA labelling, Alexa Fluor dyes Keywords: Expression profiling by array A balanced block hybridization design (Dye switch) was used where half of the samples were labelled with Alexa® 555 fluorescent dye and the other half with Alexa® 647. A total of 16 microarray slides were used for hybridization to 32 samples that correspond to four tissue contrast (White isthmus versus magnum and uterus versus white isthmus)....

  10. Deciphering the Cancer Cell Resistome: Gene and microRNA Expression Signatures reveal Unique Molecular Targets for Therapeutic Intervention in Etoposi... ArrayExpress

    ID: E-GEOD-28415

    Description: tial expression of over 5000 genes (fold change > 2, P value < 0.05) indicate that several drug resistance mechanisms may be operating in these cells, including up-regulation of ABC transporter genes, down-regulation of the drug target and down-regulation of apoptotic genes. Several transcription factors such as RUNX2, SOX9, ETS1 and SMAD3 were up-regulated in the drug resistant cells. Targeted RUNX2 knockdown in the resistant cells using siRNA increased sensitivity to etoposide and also upregulated expression of pro-apoptotic genes indicating that RUNX2 could be a molecular target against etoposide resistance. Differential miRNA (microRNA) expression was observed among the drug resistant and sensitive cells suggesting that miRNA may also play a ro...

  11. Skeletal muscle gene expression after myostatin knockout in mature mice BioProject

    ID: PRJNA116581

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ression of sets of genes with promoter motifs for Smad3, Smad4, myogenin, NF-κB, serum response factor, and numerous other transcription factors. Main conclusions: in mature muscle, myostatin is a key transcriptional regulator of collagen genes, but not genes encoding contractile proteins or genes encoding proteins involved in energy metabolism. Overall design: Comparison of muscle gene expression in 5 mice with postdevelopmental myostatin knockout and 5 control mice...
  12. TG-interacting factor1 (Tgif1) maintains the identity of mouse ES cells by counterbalancing the expression of ES cell core factors BioProject

    ID: PRJNA239579

    Keywords: Epigenomics

    Access Type: download

    dataset.description: scription factors (Tgif1, Tgif2, Nanog, Hdac1 and Smad3) in mouse ES cells. Nanog ChIP was performed using bioChIP-seq. Control ChIP-seq was performed using BirA cells....
  13. Mus musculus : Mus musculus Raw sequence reads BioProject

    ID: PRJNA354803

    Keywords: raw sequence reads

    Access Type: download

    dataset.description: (bHLH) transcription factor, which is induced by Smad3 under TGF-β stimulation, is not only critical for iTreg cell development but promotes development of iTregs by repressing the Th2 transcriptional program. Loss of MSC reduces Foxp3 expression and induces Th2 differentiation even under TGF-β induced iTreg differentiation conditions. MSC mediates this effect by physically interacting with GATA3, by interrupting binding of GATA3 to the Th2 cytokine locus and by reducing intrachromosomal interactions within the Th2 cytokine gene cluster. iTregs from MSC-deficient mice are not able to suppress Th2 responses and the Msc–/– mice spontaneously develop gut and lung inflammation with age. Our data indicate that MSC enforces Foxp3 expression and promotes un...
  14. A growth differentiation factor 9 (GDF9) motif regulates follicle-stimulating hormone b-subunit (FSHb) gene expression: involvement of activin recepto... BioProject

    ID: PRJNA229506

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: expression. Treatment with GDF9 stimulated Smad2/3 phosphorylation. The activin receptor-like kinase (ALK) receptor inhibitor SB-505124 antagonized GDF9-induced Smad2/3 phosphorylation and FSHb mRNA induction. Smad2 and Smad3 knockdown studies indicated that the induction of FSHb by GDF9 involves both Smad2 and Smad3. GDF9 and GnRH synergistically induced FSHb mRNA expression and high frequency GnRH pulses suppressed GDF9. We hypothesized that GDF9 contributes to a regulatory loop that tunes the GnRH frequency-response characteristics of the FSHb gene. To test this, we determined the effects of GDF9 knockdown on FSHb induction at different GnRH pulse frequencies using a parallel perifusion system. Reduction of GDF9 shifted the characteristic pattern of GnRH pulse frequency sensitivity. These results identify GDF9 as contributing to an incoheren...
  15. Activin/Nodal signalling controls divergent transcriptional networks in pluripotent and endoderm progenitors. BioProject

    ID: PRJNA120331

    Keywords: Epigenomics

    Access Type: download

    dataset.description: iptional network controlled in hESCs by Smad2 and Smad3 which represent the direct effectors of Activin/Nodal signalling. These analyses reveal that Smad2/3 participate in the control of the core transcriptional network characterising pluripotency which includes Oct-4, Nanog, FoxD3, Dppa4, Tert, Myc and UTF-1. In addition, similar experiments performed on endoderm cells confirm that a broad part of the transcriptional network directing differentiation is downstream of Smad...
  16. Chromatin and Transcriptional Signatures for Nodal Signaling During Endoderm Formation in hESCs BioProject

    ID: PRJNA141533

    Keywords: Epigenomics

    Access Type: download

    dataset.description: ed ChIP-Seq to identify genomic targets for SMAD2/3, SMAD3, SMAD4, FOXH1 and the active and repressive chromatin marks, H3K4me3 and H3K27me3, in human embryonic stem cells (hESCs) and derived endoderm. We demonstrate that while SMAD2/3
  17. Genomic differences distinguish the myofibroblast phenotype of distal lung from airway fibroblasts BioProject

    ID: PRJNA137313

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: tween AF and DLF, including identification of the SMAD3 and MAPK8 signaling pathways. These results demonstrated that marked molecular and functional differences exist between these two lung regional fibroblast populations. These striking differences identify multiple potential mechanisms by which AF and DLF differ in their responses to injury, regeneration and remodeling in the lungs. Overall design: In order to better identify the underlying molecular differences between AF and DLF, microarray analysis was performed on 12 different matched pairs of fibroblasts (4 pairs from normal subjects and 8 pairs from asthmatics)....
  18. Skeletal muscle gene expression after myostatin knockout in mature mice GEMMA

    ID: 1238

    Keywords: functional genomics

    Description: ression of sets of genes with promoter motifs for Smad3, Smad4, myogenin, NF-?B, serum response factor, and numerous other transcription factors. Main conclusions: in mature muscle, myostatin is a key transcriptional regulator of collagen genes, but not genes encoding contractile proteins or genes encoding proteins involved in energy metabolism. Last Updated (by provider): Mar 23 2009 Contributers: Stephen Welle...

  19. Expression profile of etoposide-resistant MCF7 (MCF7VP) cells ArrayExpress

    ID: E-GEOD-28413

    Description: tial expression of over 5000 genes (fold change > 2, P value < 0.05) indicate that several drug resistance mechanisms may be operating in these cells, including up-regulation of ABC transporter genes, down-regulation of the drug target and down-regulation of apoptotic genes. Several transcription factors such as RUNX2, SOX9, ETS1 and SMAD3 were up-regulated in the drug resistant cells. Targeted RUNX2 knockdown in the resistant cells using siRNA increased sensitivity to etoposide and also upregulated expression of pro-apoptotic genes indicating that RUNX2 could be a molecular target against etoposide resistance. Differential miRNA (microRNA) expression was observed among the drug resistant and sensitive cells suggesting that miRNA may also play a role i...

  20. Invasion control by a positive feedback loop mechanism Smad4-null cells BioProject

    ID: PRJNA101547

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: al factors alternative to Smad4 can bind to Smad2/3 and mediate different transcriptional effects. In this study, we detected constitutively phosphorylation of Smad2/3 in Smad4-null pancreatic cancer cell ...

Displaying 20 of 213 results for "SMAD3"