microRNA-146b improves intestinal injury in mouse colitis by activating NF-kB and improving epithelial barrier function OmicsDI

ID: E-GEOD-40708

Date Released: 09-26-2012

Description: ear. The present study investigated the effect of microRNA (miR-146b) on improving intestinal inflammation. Methods: The microRNA profile in IL-10-deficient mice was examined using

Modulation of mucosal immune responses to Clostridium difficile by peroxisome proliferator-activated receptor γ and microRNA-146b BioProject

ID: PRJNA170259

Keywords: Transcriptome or Gene expression

Access Type: download

microRNA-146b improves intestinal injury in mouse colitis by activating NF-kB and improving epithelial barrier function BioProject

ID: PRJNA174740

Keywords: Transcriptome or Gene expression

Access Type: download

MicroRNA profiling of bleomycin-induced pulmonary fibrosis in mice ArrayExpress

ID: E-GEOD-45789

Description: y and hyperplasia. To determine if alterations in microRNA expression contribute to these phenotypes, microRNA expression profiling of the lungs from bleomycin treated C57Bl/6J mice, relative to that of untreated controls, was undertaken. Mice were treated at 8 weeks old with 100 Units/kg of bleomycin delivered subcutaneou...

microRNA-146b improves intestinal injury in mouse colitis by activating NF-kB and improving epithelial barrier function ArrayExpress

ID: E-GEOD-40708

Description: ear. The present study investigated the effect of microRNA (miR-146b) on improving intestinal inflammation. Methods: The microRNA profile in IL-10-deficient mice was examined using

MicroRNA-146 function in the innate immune response of zebrafish embryos to Salmonella typhimurium infection [RNA-seq] OmicsDI

ID: E-GEOD-45209

Date Released: 10-03-2013

Description: an in vivo system to investigate the role of the microRNA-146 family (consisting of 2 members miR-146a and miR-146b) in the innate immune response to S...

MicroRNA-146 function in the innate immune response of zebrafish embryos to Salmonella typhimurium infection [RNA-seq] ArrayExpress

ID: E-GEOD-45209

Description: an in vivo system to investigate the role of the microRNA-146 family (consisting of 2 members miR-146a and miR-146b) in the innate immune response to S...

MicroRNA-146 function in the innate immune response of zebrafish embryos to Salmonella typhimurium infection [RNA-seq] BioProject

ID: PRJNA193208

Keywords: Transcriptome or Gene expression

Access Type: download

The miR-146b-3p/PAX8/NIS Regulatory Circuit Modulates the Differentiation Phenotype and Function of Thyroid Cells During Carcinogenesis. OmicsDI

ID: E-GEOD-63511

Date Released: 08-29-2015

Description: PTC. The integrated analyses identified a master microRNA regulatory network in PTC that is involved in essential biological processes such as thyroid differentiation. As both mature products of miR-146b (miR-146b-5p and -3p) were among the most abundant upregulated in tumors, we unveil their target genes and found that miR-146b-3p specifically binds to the 3`UTR of PAX8 and NIS, leading to an impaired translation of the proteins and subsequently decreasing the iodide uptake of the cells. Furthermore, we show that mir-146b and PAX8 regulate each other, describing a novel regulatory circuit that determines the differentiated phenotype of PTC. In conclusion, our integrative genomic analysis uncovers the target genes of two of the most upregulated miRNAs and highlights the importanc...

The miR-146b-3p/PAX8/NIS Regulatory Circuit Modulates the Differentiation Phenotype and Function of Thyroid Cells During Carcinogenesis ArrayExpress

ID: E-GEOD-63511

Description: PTC. The integrated analyses identified a master microRNA regulatory network in PTC that is involved in essential biological processes such as thyroid differentiation. As both mature products of miR-146b (miR-146b-5p and -3p) were among the most abundant upregulated in tumors, we unveil their target genes and found that miR-146b-3p specifically binds to the 3`UTR of PAX8 and NIS, leading to an impaired translation of the proteins and subsequently decreasing the iodide uptake of the cells. Furthermore, we show that mir-146b and PAX8 regulate each other, describing a novel regulatory circuit that determines the differentiated phenotype of PTC. In conclusion, our integrative genomic analysis uncovers the target genes of two of the most upregulated miRNAs and highlights the importanc...

The miR-146b-3p/PAX8/NIS Regulatory Circuit Modulates the Differentiation Phenotype and Function of Thyroid Cells During Carcinogenesis BioProject

ID: PRJNA268095

Keywords: Transcriptome or Gene expression

Access Type: download

MicroRNA-profiling by high-throughput sequencing of colonic tissues from wild-type C57BL6/J mice with or without infection by Clost... ArrayExpress

ID: E-GEOD-39235

Description: h Clostridium difficile strain VPI 10463 RESULTS: MicroRNA (miRNA)-sequencing analysis indicated that miR-146b, miR-1940, and miR-1298 were significantly overexpressed in colons of C. difficile-infected mice Colon of uninfected and C.difficile-infected C57BL6/J WT mice were sampled at day 4 post-infection with Clostridium difficile VPI 1046...

MicroRNA-profiling by high-throughput sequencing of colonic tissues from wild-type C57BL6/J mice with or without infection by Clost... OmicsDI

ID: E-GEOD-39235

Date Released: 01-13-2015

Description: h Clostridium difficile strain VPI 10463 RESULTS: MicroRNA (miRNA)-sequencing analysis indicated that miR-146b, miR-1940, and miR-1298 were significantly overexpressed in colons of C. difficile-infected mice Colon of uninfected and C.difficile-infected C57BL6/J WT mice were sampled at day 4 post-infection with Clostridium difficile VPI 1046...

MicroRNA profiling of bleomycin-induced pulmonary fibrosis in mice OmicsDI

ID: E-GEOD-45789

Date Released: 05-13-2014

Description: y and hyperplasia. To determine if alterations in microRNA expression contribute to these phenotypes, microRNA expression profiling of the lungs from bleomycin treated C57Bl/6J mice, relative to that of untreated controls, was undertaken. Mice were treated at 8 weeks old with 100 Units/kg of bleomycin delivered subcutaneou...

MicroRNA Profiling Analysis of Differences between the Melanoma of Young Adults and Older Adults BioProject

ID: PRJNA120857

Keywords: Transcriptome or Gene expression

Access Type: download

MicroRNA transcriptome in weaning and sucking porcine intestines ArrayExpress

ID: E-GEOD-50500

Description: xpressed miRNAs were found between W4 and S4 (ssc-mir-146b had the largest difference and ssc-mir-215 had the highest expression level); and 22 differentially expressed miRNAs were found between W7 and S7. Sequencing miRNA results were validated using RT-qPCR. Approximately 12,819 miRNA-mRNA interactions corresponding to 4,250 target genes were predicted. The biological analyses revealed that the differentially expressed miRNAs regulated small intestinal metabolism, stressful responses, cellular and immune functions and miRNA biosynthesis in piglets. Therefore, the small intestine miRNA transcriptome was significantly different between weaning and suckling piglets; the difference varied with the number of days after weaning. six small RNA libraries from weaning piglets at 1, 4 and 7 d after weaning ...

MicroRNA profiling of bleomycin-induced pulmonary fibrosis in mice BioProject

ID: PRJNA196321

Keywords: Transcriptome or Gene expression

Access Type: download

MicroRNA transcriptome in weaning and sucking porcine intestines BioProject

ID: PRJNA217780

Keywords: Transcriptome or Gene expression

Access Type: download

Altered microRNA expression and pre-mRNA splicing events reveal new mechanisms associated with early stage Mycobacterium avium subspecies p... BioProject

ID: PRJNA300273

Keywords: Transcriptome or Gene expression

Access Type: download

MicroRNA profiling by array of 217 cases of chronic lymphocytic leukemia (CLL) comparing CLL-affected B-cells against normal B-cell... OmicsDI

ID: E-MTAB-1454

Date Released: 05-03-2014

Description: rentially expressed miRNAs in CLL, which included miR-193b, miR-33b*, miR-365, miR-181b and miR-196a/b among the down-regulated and miR-23a/b, miR-26a, miR-130a, miR-532 (5p and 3p) among the up-regulated miRNAs. We also investigated differences of miRNA expression related to the IGHV somatic mutation status and to deletions at 13q, 11q and 17p or trisomy 12. Little differences were detected between unmutated (UM) versus mutated (M) CLLs, although miR-29c, miR-29c* and miR-146b were st...

PPARG and microRNA-146 in mucosal immune responses to C. difficile ImmPort

ID: SDY100

Description: een and colonic lamina propria of mice with CDAD. MicroRNA (miRNA)-sequencing analysis and RT-PCR validation indicated that miR-146b was significantly overexpressed in colons of C. difficile-infected mice. We next developed a computational model that predicts the upregulation of miR-146b, downregulation of the PPAR gamma co-activator NCOA4, and PPAR gamma, leading to upregulation of IL-17. Treatment of C. difficile-infected mice with the PPAR gamma agonist pioglitazone ameliorated colitis and suppressed pro-inflammatory gene expression. In conclusion, our data indicates that miRNA-146b and PPAR gamma may be implicated in the regulation of Th17 responses and colitis in C. difficile-infected mice....

MicroRNA profiling by array of 217 cases of chronic lymphocytic leukemia (CLL) comparing CLL-affected B-cells against normal B-cell... ArrayExpress

ID: E-MTAB-1454

Description: rentially expressed miRNAs in CLL, which included miR-193b, miR-33b*, miR-365, miR-181b and miR-196a/b among the down-regulated and miR-23a/b, miR-26a, miR-130a, miR-532 (5p and 3p) among the up-regulated miRNAs. We also investigated differences of miRNA expression related to the IGHV somatic mutation status and to deletions at 13q, 11q and 17p or trisomy 12. Little differences were detected between unmutated (UM) versus mutated (M) CLLs, although miR-29c, miR-29c* and miR-146b were st...

Aberrantly microRNA profiling in cancerous hepatocytes versus normal primary hepatocytes BioProject

ID: PRJNA124197

Keywords: Transcriptome or Gene expression

Access Type: download

Human Corneal microRNA Expression Profile in Fungal Keratitis BioProject

ID: PRJNA272148

Keywords: Transcriptome or Gene expression

Access Type: download

MicroRNA transcriptome in weaning and sucking porcine intestines OmicsDI

ID: E-GEOD-50500

Date Released: 09-07-2014

Description: xpressed miRNAs were found between W4 and S4 (ssc-mir-146b had the largest difference and ssc-mir-215 had the highest expression level); and 22 differentially expressed miRNAs were found between W7 and S7. Sequencing miRNA results were validated using RT-qPCR. Approximately 12,819 miRNA-mRNA interactions corresponding to 4,250 target genes were predicted. The biological analyses revealed that the differentially expressed miRNAs regulated small intestinal metabolism, stressful responses, cellular and immune functions and miRNA biosynthesis in piglets. Therefore, the small intestine miRNA transcriptome was significantly different between weaning and suckling piglets; the difference varied with the number of days after weaning. six small RNA libraries from weaning piglets at 1, 4 and 7 d after weaning ...

Human Corneal microRNA Expression Profile in Fungal Keratitis OmicsDI

ID: E-GEOD-64843

Date Released: 07-28-2016

Description: lect target genes were further analysed by Q-PCR. MiR-21-5p, miR-223-3p, miR-146b-5p, miR-155-5p, miR-511-5p were found to be involved in inflammatory and immune responses, regulating Toll like receptor signaling pathways, which is of particular interest. MiR-451a with an increased expression in keratitis may have a role in wound healing by targeting Macrophage Migration Inhibitory Factor (MIF). Further, we highlighted that Neurotrophin signaling pathway may play a role in wound healing process. One novel miRNA was also detected in cornea. In conclusion, several miRNAs with high expression in fungal keratitis corneas point towards their role in regulation of pathogenesis. Further insights in understanding miRNAs role in wound healing and inflammation may help design new therapeutic strategies. Control and fungal keratitis, human corneal miRNA profiles were generated using IlluminaHiseq1000 platform...

New global miRSeq analysis of “miRNome”: the example of PTC and nodal metastases BioProject

ID: PRJNA248083

Keywords: Transcriptome or Gene expression

Access Type: download

MicroRNA analysis of the bovine alveolar macrophage response to Mycobacterium bovis infection suggests pathogen survival is enhance... BioProject

ID: PRJNA253809

Keywords: Transcriptome or Gene expression

Access Type: download

miRNA expression profiling in liver of juvenile and egg-laying hens BioProject

ID: PRJNA299589

Keywords: Transcriptome or Gene expression

Access Type: download

Human Corneal microRNA Expression Profile in Fungal Keratitis ArrayExpress

ID: E-GEOD-64843

Description: lect target genes were further analysed by Q-PCR. MiR-21-5p, miR-223-3p, miR-146b-5p, miR-155-5p, miR-511-5p were found to be involved in inflammatory and immune responses, regulating Toll like receptor signaling pathways, which is of particular interest. MiR-451a with an increased expression in keratitis may have a role in wound healing by targeting Macrophage Migration Inhibitory Factor (MIF). Further, we highlighted that Neurotrophin signaling pathway may play a role in wound healing process. One novel miRNA was also detected in cornea. In conclusion, several miRNAs with high expression in fungal keratitis corneas point towards their role in regulation of pathogenesis. Further insights in understanding miRNAs role in wound healing and inflammation may help design new therapeutic strategies. Control and fungal keratitis, human corneal miRNA profiles were generated using IlluminaHiseq1000 platform...

Paired non-cancerous and cancerous miRNA expression of esophageal adenocarcinoma and squamous cell carcinoma patients ArrayExpress

ID: E-GEOD-13937

Description: enrolled in 3 distinct cohorts. In ADC patients, miR-21, miR-194, miR-293, and miR-223 expression was elevated, while miR-375 and miR-203 expression was reduced in cancerous tissue compared to non-cancerous tissue. Increased levels of miR-192 and miR-194 were observed in Barrett’s associated compared to sporadic ADC cancerous tissue. In SCC patients, miR-21, miR-181b, miR-155, and miR-146b expression was elevated while miR

Immune responses to the in vitro LPS assault engineered in the spaceflight multi-omics study OmicsDI

ID: E-GEOD-54213

Date Released: 06-03-2014

Description: compelled further investigation of the respective microRNA modulators such as miR-200a and miR-146b. These miRNAs were expressed differently in response to LPS assaults in different gravitational limits. In conclusion, despite a serious drawback attributed to the small sample size, we delineated some of the important aspects of the extraterrestrial etiology; more comprehensive follow up studies are warranted. Present study though compromised by the small sample size was able to shade lights on several aspects of immunological responses to the endotoxic assault mediated by μG. Implementing the host-pathogen interactions in the spaceflight and subsequently lysing the cells onboard presented the critical distinguishing features of the present study from the past reports. We identified the CCM of Tissue Genesis, Inc., HI as the suitable hardware system to carry out the experiment in the spaceflight. CCM is an automated, feedback controlled module that can concurrently support 24 bioreactors following protocols exclusively programmed for individual bioreactor. For this experiment we use samples EA41, EA 47, EA45, and EA155 that were exposed to LPS for 4 hours. Samples EA123, EA165, EA127, EA126 were exposed to LPS for 8Hrs. Samples EA33, EA 125, EA79 and EA 39 were controls in this experiment....

Immune responses to the in vitro LPS assault engineered in the spaceflight multi-omics study ArrayExpress

ID: E-GEOD-54213

Description: compelled further investigation of the respective microRNA modulators such as miR-200a and miR-146b. These miRNAs were expressed differently in response to LPS assaults in different gravitational limits. In conclusion, despite a serious drawback attributed to the small sample size, we delineated some of the important aspects of the extraterrestrial etiology; more comprehensive follow up studies are warranted. Present study though compromised by the small sample size was able to shade lights on several aspects of immunological responses to the endotoxic assault mediated by μG. Implementing the host-pathogen interactions in the spaceflight and subsequently lysing the cells onboard presented the critical distinguishing features of the present study from the past reports. We identified the CCM of Tissue Genesis, Inc., HI as the suitable hardware system to carry out the experiment in the spaceflight. CCM is an automated, feedback controlled module that can concurrently support 24 bioreactors following protocols exclusively programmed for individual bioreactor. For this experiment we use samples EA41, EA 47, EA45, and EA155 that were exposed to LPS for 4 hours. Samples EA123, EA165, EA127, EA126 were exposed to LPS for 8Hrs. Samples EA33, EA 125, EA79 and EA 39 were controls in this experiment....

Homo sapiens : High-throughput analysis of miRNA expression profile in Hepatocellular Carcinoma using Next Generation Sequencing BioProject

ID: PRJDB2795

Keywords: Other

Access Type: download

Immune responses to the in vitro LPS assault engineered in the spaceflight multi-omics study BioProject

ID: PRJNA237328

Keywords: Transcriptome or Gene expression

Access Type: download