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Displaying 20 of 250 results for "GFAP"
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  1. PDGF Engages an E2F-USP1 Signaling Pathway to Support ID2-mediated Survival of Proneural Glioma Cells. OmicsDI

    ID: E-GEOD-77419

    Date Released: 03-26-2016

    Description: cells (PDGF-GSC) which had been isolated from the Gfap-tTa/Tre-PDGFB mouse model used in our study. The data were analyzed to determine the genes that are regulated by PDGF signaling in PDGF-driven glioma....

  2. Upregulated Hedgehog Signaling Promotes Zebrafish CNS Tumorigenesis OmicsDI

    ID: E-GEOD-40761

    Date Released: 06-02-2014

    Description: escence studies confirmed overexpression of Pax2, GFAP, S100 and Sox2, specifically in gliomas. We also detected upregulated expression of phosphorylated pRb and Mdm2 associated with gliomagenesis. Our results suggest that dysregulated Hh signaling initiates tumorigenesis in zebrafish CNS. Zebrafish optic nerve maintains phosphorylated pRb expression, therefore provides a unique niche for pathogenesis of glioma. RNAs from 4 gross eye tumors (from fish of 4 months of age) and 4 normal eyes from wil-type control fish were extracted and used for array a...

  3. Expression data from MRTFA-B double knockout brain OmicsDI

    ID: E-GEOD-22117

    Date Released: 05-02-2014

    Description: -/-;MRTF-BFlox/Flox and three MRTF-A-/-;MRTF-BF/F;GFAP-Cre RNA samples were collected and pooled for hybridization on Affymetrix microarrays....

  4. Rosette-forming glioneuronal tumour arising in the spinal cord: a case report and molecular profiling OmicsDI

    ID: E-GEOD-64891

    Date Released: 08-19-2015

    Description: reas the glial component exhibited positivity for GFAP and S-100. Neurocytic component was found negative for GFAP. Mitoses were rare, and there was no necrosis present. Across all mutational analyses, there were detected somatic mutations in 4 genes: MLL2, CNNM3, PCDHGC4 and SCN1A. The tumor exhibited a microsatellite stable (MSS) phenotype. Array-CGH (aCGH) showed loss in 1p and gain of 1q, as well as gain of the whole chromosomes 7, 9 and 16. Moreover, we observed focal gains/losses in the chromosomes 1, 2, 3, 6, 7, 11, 14, 17, 22 and 23. Local amplifications in 9q34.2 (region with no genes mapped) and 19p13.3 (region encompassing the gene SBNO2) were identified. Additionally, we verified the KIAA1549-BRAF fusion, typically found in pilocytic astrocytomas, through aCGH, RT-PCR and FISH. Our comprehensive molecular profiling of a RGNT case suggests the existence of a unique genetic pathway for the development of these tumors: KIAA1549-BRAF fusion is a possible driver by constitutively activating MAPK pathway, and MLL2 mutation may lead to profound c...

  5. Transcription profiling of mouse conditional knockout of patched (mouse model of medulloblastoma) to investigate the control initiation and progressio... OmicsDI

    ID: E-GEOD-12430

    Date Released: 03-27-2012

    Description: loblastomas initiated in neural stem cells (using GFAP-Cre) are more aggressive than those initiated in neural progenitors (using Math1-Cre), and will express more genes associated with self-renewal. In addition, we hypothesize that cancer stem cells (CD15+ cells) from a given tumor will express higher levels of self-renewal genes than non-stem cells (CD15- cells) from the same tumor. Cells from 5 GFAP-Cre tumors, 5 Math1-Cre tumors, and 5 conventional patched-knockout tumors will be isolated using enzymatic digestion and Percoll gradient centrifugation. These procedures have been found to result in 85-95% pure tumor cell populations. In addition, 5 tumors will be FACS-sorted to isolate CD15+ and CD15- populations. RNA will be prepared from each of these samples using an RNeasy kit from Qiagen. Samples of 2 micrograms will be resuspended in 10 microliters of RNase-free water, and sent to the Consortium for labeling, hybridization and analysis. Cells from the three types of tumors, and CD15+ and CD15- cells, will be compared to one another....

  6. Loss of PATCHED (wechs-affy-mouse-512645) GEMMA

    ID: 1104

    Keywords: functional genomics

    Description: loblastomas initiated in neural stem cells (using GFAP-Cre) are more aggressive than those initiated in neural progenitors (using Math1-Cre), and will express more genes associated with self-renewal. In addition, we hypothesize that cancer stem cells (CD15+ cells) from a given tumor will express higher levels of self-renewal genes than non-stem cells (CD15- cells) from the same tumor. Cells from 5 GFAP-Cre tumors, 5 Math1-Cre tumors, and 5 conventional patched-knockout tumors will be isolated using enzymatic digestion and Percoll gradient centrifugation. These procedures have been found to result in 85-95% pure tumor cell populations. In addition, 5 tumors will be FACS-sorted to isolate CD15+ and CD15- populations. RNA will be prepared from each of these samples using an RNeasy kit from Qiagen. Samples of 2 micrograms will be resuspended in 10 microliters of RNase-free water, and sent to the Consortium for labeling, hybridization and analysis. Cells from the three types of tumors, and CD15+ and CD15- cells, will be compared to one another. Last Updated (by provider): Feb 09 2009 Contributers: Robert J Wechsler-Reya...

  7. Transcription profiling by array of mouse developing ureters ArrayExpress

    ID: E-MEXP-3929

    Description: The mammalian ureter contains a water-tight epithelium surrounded by smooth muscle. Key molecules have been defined which regulate ureteric bud initia...

  8. Effects of Hsp72 on astrocyte activation following stroke in the mouse ArrayExpress

    ID: E-GEOD-28731

    Description: tent increase in glial fibrillary acidic protein (GFAP ) as soon as 3 hours after MCAO. In contrast, vimentin immunoreactivity appeared 12-24 hours after stroke, peaked at 72 hours, and returned to baseline after 30 days. Surprisingly, no change in overall astrocyte number was observed based on glutamine synthetase (GS) immunoreactivity. To determine if Hsp72Tg mice exhibited altered astrocyte activation compared to WT controls, morphological evaluation by fractal analysis was used. Overexpression of Hsp72 reduced astrocyte cell area, arbor area, and to a lesser extent fractal dimension, 72 hours following stroke. In conclusion, in vivo overexpression of Hsp72 alters gene expression following stroke, including genes involved in astrocyte activation, and decreases astrocyte activation acutely following MCAO. Thus, modulation of astrogliosis may be a neuroprotective mechanism exerted by Hsp72 after ischemia. A total of 10 samples were analyzed, with 5 of each genotype, wildtype (WT) and Hsp72-overexpressing (Hsp72Tg) mice. Of the 5 in each group, 3 received middle cerebral artery occlusion (MCAO) and 2 rece...

  9. Transcription profiling of mouse models or Parkinsons disease reveal miRNAs and codon usage regulate striatal gene and protein expression ArrayExpress

    ID: E-GEOD-8030

    Description: ch may be involved in the pathophysiology of PD. GFAP transcript and protein levels were significantly up-regulated by both neurotoxins, confirming the known astrocytic response to these drugs. Other genes and proteins were idiosyncratic in their responses to the two toxins, suggesting specific toxicological responses. Comparing transcript and protein levels revealed that efficiently translated genes used more commonly occurring codons than inefficiently translated genes. Additionally, a potential role was found for miRNAs in translational control in the striatum. The results constitute one of the largest datasets integrating transcript and prot...

  10. Transcriptional Profile Analysis of RPGRORF15 frameshift mutation ArrayExpress

    ID: E-GEOD-19124

    Description: on for additional genes not on the array, such as GFAP, RHO, OPN1SW, CNGB3 and the mutated RPGR. Western blotting and IHC analysis also confirmed the high reliability of the presented transcriptomic data. Conclusions: A list of mutated genes in RPGRORF15 diseased retinas, which are likely candidates to further study their role in age-related photoreceptor degeneration diseases, is reported at different crucial ages. The results indicate that at 7 weeks a combination of non-classical anti- and pro-apoptotic genes appears to be involved in photoreceptor degeneration, whereas at both 7 and 16 weeks expression of mitochondria related genes indicates they may play a relevant role in the disease process. 3 biological replicates each for normal and XLPRA2 affected retinas were analyzed at 7 and 16 weeks of age. Each individual sample was hybridized in a reference design using a custom-made retinal cDNA microarray against brain pool to enable cross comparison between groups. Retina was labeled with Cy5 in all samples but 5615 (GSM474350)....

  11. Integrative functional genomic analysis identifies epigenetically regulated Fibromodulin as an essential gene for glioma cell migration ArrayExpress

    ID: E-GEOD-79122

    Description: med using EGFR (monoclonal E-30, diluted to 1:50) GFAP status: Paraffin blocks of all the cases were retrieved, and histological features were reviewed on freshly cut and stained (hematoxylin and eosin) sections. After confirming the diagnosis of glioblastoma, 4 µm thick sections were collected on silane-coated slides, and IHC was performed using glial fibrillary acidic protein (GFAP, monoclonal GA-5, diluted to 1:100)....

  12. PDGF Engages an E2F-USP1 Signaling Pathway to Support ID2-mediated Survival of Proneural Glioma Cells. BioProject

    ID: PRJNA310247

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: cells (PDGF-GSC) which had been isolated from the Gfap-tTa/Tre-PDGFB mouse model used in our study. The data were analyzed to determine the genes that are regulated by PDGF signaling in PDGF-driven glioma....
  13. PDGF Engages an E2F-USP1 Signaling Pathway to Support ID2-mediated Survival of Proneural Glioma Cells. BioProject

    ID: PRJNA310253

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ells (PDGF-GSC) and primary tumors arising in the Gfap-tTa/Tre-PDGFB mouse model used in our study was analyzed to determine to which subtype of GBM these specimens belonged....
  14. Expression profiling of murine MYCN-driven neuroendocrine tumor from LSL-MYCN;hGFAP-Cre mice. OmicsDI

    ID: E-GEOD-73466

    Date Released: 03-05-2016

    Description: induction of MYCN in glial fibre acidic protein (GFAP) expressing cells resulting in the development of neuroendocrine tumors of the pancreas and the brain. The expression profiles of nine tumors from pancreas and three brain tumors were compared to non-malignant pancreas controls (GSM771025.CEL-GSM771027.CEL) from wildt...

  15. Transcriptome analysis of the uninjured Muller glia (MG) and MG derived retinal progenitors at 4 day post injured zebrafish retina OmicsDI

    ID: E-GEOD-36191

    Date Released: 08-20-2012

    Description: e control of the glial fibrillary acidic protein (gfap) promoter. MG-derived retinal progenitors were isolated by FACS at 4 days post retinal injury from 1016 tuba1a:gfp transgenic fish where GFP is driven by the tuba1a promoter which is specifically activated in these progenitors. Total RNA was isolated from these cell populations and subjected to microarray analysis to compare their transcriptomes. Samples were prepared in duplicate....

  16. PDGF Engages an E2F-USP1 Signaling Pathway to Support ID2-mediated Survival of Proneural Glioma Cells. OmicsDI

    ID: E-GEOD-77423

    Date Released: 03-26-2016

    Description: ells (PDGF-GSC) and primary tumors arising in the Gfap-tTa/Tre-PDGFB mouse model used in our study was analyzed to determine to which subtype of GBM these specimens belonged....

  17. Expression data of transduced mouse glioblastomas OmicsDI

    ID: E-GEOD-35917

    Date Released: 10-29-2012

    Description: Transgenic GFAP-, NES-, and SYN- CRE mice were injected with a lenti-viral construct containing a floxed RFP directly upstream of a cassette c...

  18. Integrative functional genomic analysis identifies epigenetically regulated Fibromodulin as an essential gene for glioma cell migration BioProject

    ID: PRJNA314994

    Keywords: Epigenomics

    Access Type: download

    dataset.description: med using EGFR (monoclonal E-30, diluted to 1:50) GFAP status: Paraffin blocks of all the cases were retrieved, and histological features were reviewed on freshly cut and stained (hematoxylin and eosin) sections. After confirming the diagnosis of glioblastoma, 4 µm thick sections were collected on silane-coated slides, and IHC was performed using glial fibrillary acidic protein (GFAP, monoclonal GA-5, diluted to 1:100)....
  19. PDGF Engages an E2F-USP1 Signaling Pathway to Support ID2-mediated Survival of Proneural Glioma Cells ArrayExpress

    ID: E-GEOD-77419

    Description: cells (PDGF-GSC) which had been isolated from the Gfap-tTa/Tre-PDGFB mouse model used in our study. The data were analyzed to determine the genes that are regulated by PDGF signaling in PDGF-driven glioma....

  20. Transcription profiling of mouse astroglial gene expression program elicited by fibroblast growth factor-2 ArrayExpress

    ID: E-GEOD-6675

    Description: reater than 95% of cell cultures as determined by GFAP staining. Microglia are present as a contaminating cell type at a percentage of 3.6 ± 0.9% as determined by CD11B staining. For process induction experiments, cells are trypsinized and replated in DMEM with 1% fetal bovine serum. This low serum concentration is necessary for good adhesion of the cells. Pharmacological inhibitors are dissolved in DMSO and added 2 h later. P38 MAP kinase inhibitors SB202190 (Calbiochem) and the MEK inhibitor U0126 (Promega) are used at 20 µM. We previously determined that the inactive analog of SB 202190, SB202474 (Calbiochem) had no effects on pathway activation or astrocyte morphology. Therefore, the inactive drug analog will not be used in the array experiments. Final DMSO concentration in the drug treatments and vehicle controls is 0.2%. Human recombinant FGF2 (obtained from the National Cancer Institute Preclinical Repository) is used at 25 ng/ml, based on extensive dose/response analyses in our published work....


Displaying 20 of 250 results for "GFAP"