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Displaying 20 of 250 results for "GFAP"
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  1. Oncogenic alterations in multiple core signaling pathways are required for glioblastoma pathogenesis in vitro and in vivo ArrayExpress

    ID: E-GEOD-40265

    Description: ) from the human glial fibrillary acidic protein (GFAP) promoter, which inactivates all three members of the Rb family (Rb, p107, p130) and results in proliferation and defective G1/S ratios. Additionally, cells contained KrasG12D mutant (R) and/or contained either heterozygous or homozygous deletion of Pten (P+/- or P-/-) . Here are 23 cell lines comprising 6 different genotypes. 10 Samples (Sample Title: TR_2, T_1, TRP_null_3, TR_1, TRP_null_4, TRP_het_1, T_2, TRP_null_2, T_3, TRP_null_5) from one batch were combined with the remaining 13 samples to remove batch related effects (see data processing for method)....

  2. RNAi profiling of human ezh2 specific siRNA A673 treated cells reveals EZh2 is a mediator of EWS-FLI1 driven tumor growth blocking endothelial and neu... ArrayExpress

    ID: E-GEOD-12692

    Description: in neuroectodermal differentiation (EMP1, EPHB2, GFAP, GAP43). These data suggest that EZH2 might play a central role in Ewing Tumor pathology shaping the oncogenicity and stem cell phenotype of this tumor presumably by epigenetic regulation. Experiment Overall Design: A673 cells were treated for 24 hours either with 100 nM Trichostatin A (TSA) or 0.01% DMSO. In siRNA experiments with ezh2 specific siRNA A673 cells were resuspended in medium containing 5 nM siRNA and transfection reagent and incubated for 48 hours. RNA from cells under such treatment was isolated with Trizol and subjected to microarray analysis onto human U133A microarray following the Affymetrix protocol....

  3. Murine embryonic stem cell-derived astrocytes BioProject

    ID: PRJNA106407

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: Expression profiles for Gfap-positive astrocytes obtained by in vitro differentiation of 129SvJae x C57BL/6 murine embryonic stem (ES) cells. Generated to ...
  4. Cell Image Library Dataset CIL:36414 CIL

    ID: 36414

    Description: to the astroglial-specific cytoskeletal protein, GFAP (blue). Optical sections were generated with a single photon confocal microscope, and are shown here compiled into a maximum projection image....

    Data Types: image

  5. Cell Image Library Dataset CIL:36415 CIL

    ID: 36415

    Description: to the astroglial-specific cytoskeletal protein, GFAP (blue). Optical sections were generated with a single photon confocal microscope, and are shown here compiled into a maximum projection image....

    Data Types: image

  6. Cell Image Library Dataset CIL:36421 CIL

    ID: 36421

    Description: to the astroglial-specific cytoskeletal protein, GFAP (blue). Optical sections were generated with a single photon confocal microscope, and are shown here compiled into a maximum projection image....

    Data Types: image

  7. Cell Image Library Dataset CIL:36426 CIL

    ID: 36426

    Description: zation of glial-specific proteins S-100 (red) and GFAP (blue). Optical sections were generated with a single photon confocal microscope. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database....

    Data Types: image

  8. Cell Image Library Dataset CIL:36429 CIL

    ID: 36429

    Description: zation of glial-specific proteins S-100 (red) and GFAP (blue). Optical sections were generated with a single photon confocal microscope. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database....

    Data Types: image

  9. Cell Image Library Dataset CIL:36439 CIL

    ID: 36439

    Description: zation of glial-specific proteins S-100 (red) and GFAP (blue). Optical sections were generated with a single photon confocal microscope. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database....

    Data Types: image

  10. Cell Image Library Dataset CIL:40170 CIL

    ID: 40170

    Description: lexa568 (red) respectively, and immunolabeled for GFAP (blue), imaged with confocal microscopy. Optical sections were generated with a single photon confocal microscope. Original data contributed to Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hip...

    Data Types: image

  11. miRNAs and codon usage regulate striatal gene and protein expression in two mouse models of Parkinson?s disease GEMMA

    ID: 698

    Keywords: functional genomics

    Description: ch may be involved in the pathophysiology of PD. GFAP transcript and protein levels were significantly up-regulated by both neurotoxins, confirming the known astrocytic response to these drugs. Other genes and proteins were idiosyncratic in their responses to the two toxins, suggesting specific toxicological responses. Comparing transcript and protein levels revealed that efficiently translated genes used more commonly occurring codons than inefficiently translated genes. Additionally, a potential role was found for miRNAs in translational control in the striatum. The results constitute one of the largest datasets integrating transcript and pr...

  12. Identification of therapeutic targets for glioblastoma by network analysis ArrayExpress

    ID: E-GEOD-64411

    Description: s were derived from 11 days old SynapsinI-Cre and GFAP-Cre mice, respectively. The cells were cultured in their respective media to maintain their identity. These cells were then transduced with HRas-shp53 lentivirus with a transduction efficiency of >90%. The transduced neurons and astrocytes were later switched to neural stem cell media devoid of serum and supplemented with FGF-2 (NSC media). Within one week, these cells became proliferative and aggregated to form free-floating neurospheres. These cells, hereinafter referred to as NSynR53 and AGR53, respectively, were later harvested and mRNA collected for sequencing library generation using DP-seq. To assess the regression of these cells to an undifferentiated state along the differentiation axis, enriched populations of mESC and NSC were also grown in vitro and mRNA obtained from these cells were subjected to sequencing library preparation....

  13. The connexin43-dependent transcriptome during brain development: importance of genetic background ArrayExpress

    ID: E-GEOD-37239

    Description: ome, we performed microarray studies on brains of GFAP-Cre:Cx43f/f C57Bl/6 and 129/SVEV mice. The surprisingly low number of Cx43 core genes (regulated in all Cx43 nulls regardless of strain) and the high number of differently regulated genes in the two Cx43 CKOs indicate high influence of mouse strain on brain transcriptome. The transcriptomes of WT and Cx43 null brains from both C57Bl/6 and SVEV strains were profiled and compared at perinatal and adult time points to learn more about the strain dependence of the Cx43-null phenotype. For this purpose, differently labeled cDNAs from biological replicas (4 of each genotype) were co-hybridized with Duke MO36K mouse oligonucleotide array spotted with 36k Operon oligonucleotides V4.0....

  14. Loss of PATCHED (wechs-affy-mouse-512645) BioProject

    ID: PRJNA113743

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: loblastomas initiated in neural stem cells (using GFAP-Cre) are more aggressive than those initiated in neural progenitors (using Math1-Cre), and will express more genes associated with self-renewal. In addition, we hypothesize that cancer stem cells (CD15+ cells) from a given tumor will express higher levels of self-renewal genes than non-stem cells (CD15- cells) from the same tumor. Cells from 5 GFAP-Cre tumors, 5 Math1-Cre tumors, and 5 conventional patched-knockout tumors will be isolated using enzymatic digestion and Percoll gradient centrifugation. These procedures have been found to result in 85-95% pure tumor cell populations. In addition, 5 tumors will be FACS-sorted to isolate CD15+ and CD15- populations. RNA will be prepared from each of these samples using an RNeasy kit from Qiagen. Samples of 2 micrograms will be resuspended in 10 microliters of RNase-free water, and sent to the Consortium for labeling, hybridization and analysis. Cells from the three types of tumors, and CD15+ and CD15- cells, will be compared to one another....
  15. Transcriptome Profiling of Hippocampal CA1 After Early Life Seizure-Induced Preconditioning May Elucidate New Genetic Therapies for Epilepsy BioProject

    ID: PRJNA189420

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ated (e.g., Cacng1, Nadsyn1, Kcng1, Aven, S100a4, GFAP, Vim, Hrsp12, Grik1). After 3×KA, protective genes were differentially over-expressed (e.g., Cat, Gpx7, GAD1, Hspa12A, Foxn1, adenosine A1 receptor, Ca2+ adaptor and homeostatic proteins, Cacnb4, Atp2b2, anti-apoptotic Bcl-2 gene members, intracellular trafficking protein, Grasp, suppressor of cytokine signaling (Socs3)). Distinct anti-inflammatory interleukins not observed in adult tissues (e.g., IL6 transducer, IL23 and IL33 or their receptors (ILF2)) were also over-expressed. Several transcripts were validated by real-time polymerase chain reaction (QPCR) and immunohistochemistry. QPCR showed that casp 6 was increased after 1×KA but reduced after 3×KA; pro-inflammatory gene cox1 was either upregulated or unchanged after 1...
  16. miRNAs and codon usage regulate striatal gene and protein expression in two mouse models of Parkinson’s disease BioProject

    ID: PRJNA100839

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ch may be involved in the pathophysiology of PD. GFAP transcript and protein levels were significantly up-regulated by both neurotoxins, confirming the known astrocytic response to these drugs. Other genes and proteins were idiosyncratic in their responses to the two toxins, suggesting specific toxicological responses. Comparing transcript and protein levels revealed that efficiently translated genes used more commonly occurring codons than inefficiently translated genes. Additionally, a potential role was found for miRNAs in translational control in the striatum. The results constitute one of the largest datasets integrating transcript and prot...
  17. Transgenic inhibition of astroglial NF-kappaB in experimental autoimmune encephalomyelitis GEMMA

    ID: 2132

    Keywords: functional genomics

    Description: nic inactivation of astroglial NF-kappaB in mice (GFAP-IkappaBalpha-dn mice) resulted in dramatic reduction of disease severity and improvement in functional recovery following EAE. This coincided with a higher presence of leukocytes in the cord and brain of transgenic mice at the chronic phase of the disease, when the functional recovery over WT mice was the most significant. We observed that expression of proinflammatory genes in both spinal cord and cerebellum was delayed and reduced, while ...

  18. Data for figures 2 to 6.xlsx Figshare

    ID: doi:10.6084/M9.FIGSHARE.4256576.V1

    Release Date: 11-24-2016

    Description: ence from baseline for a-wave and b-wave, ONL and GFAP quantification.


    ...

  19. Expression profiling of glioma initiating cells (GICs) in the sphere and differentiation conditions ArrayExpress

    ID: E-GEOD-43762

    Description: e down-regulated, and the astrocyte/glioma marker GFAP and the malignancy marker CD44 dramatically up-regulated. To identify genes/proteins whose expression changes dynamically during the differentiation of GICs into glioma cells, these GICs were subjected to DNA microarray/iTRAQ based integrated proteomics. Within 4 hours of tumor removal from GBM patients, tissues were subjected to GIC preparation. After successive cloning, total RNA from GIC clones (GIC03A and GIC03U) on day 2 or 7 of subculture in NSC medium with or without 10% FCS was subjected to the analysis with Affymetrix microarrays. Simultaneously, the proteins extracted from the same set of cells were subjected to LC-shot gun analyses using the 8-plex iTRAQ method. We sought to obtain the information of the common molecules that were up- or down-regulated d...

  20. Matrix metalloproteinase-9 and TIMP-1 in peripheral nerve: Implications for myelin formation ArrayExpress

    ID: E-GEOD-33454

    Description: 9 gene deletion increased the number of immature, GFAP+ mSC and post-mitotic cell counts that correlate with shorter myelin internodes in remyelinated fibers lacking MMP-9. MMP-9 is essential to nodal clustering of voltage-gated Na+ (Nav) channels. MMP inhibitor therapy diminished the expression of Nav 1.7 and 1.8. These data established the essential role of MMP-9 in guiding SC differentiation toward myelin production and in molecular assembly of the myelin domains. Modification of Nav channels in myelinated fibers may thus provide an important therapeutic approach for a number of facilitates regeneration and attenuated neuropathic pain. Gene expression profiling of total RNAs extracted from murine sciatic nerves, dorsal root ganglion and spinal cords at day 1 and day 5 post injury....


Displaying 20 of 250 results for "GFAP"