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Displaying 20 of 250 results for "GFAP"
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  1. Expression data from adult Shh-responding astroglia cells in mice ArrayExpress

    ID: E-GEOD-50950

    Description: e expression data obtained from FACS-sorted Gli1+ GFAP+ cells from microdissected SVZ, hippocampus and cerebellum. GFAP expression is based on hGFAP-GFP reporter line and Gli1 expression is lineage marked using Gli1-CreER;ROSA26-tdTomato mice. 15 Total samples were analyzed. We compared expression levels of SVZ vs. Cerebellum, Hippocampus vs. Cerebelllum to identify genes which had more than 4 fold change in expression levels with p < 0.01. From this narrowed list, we compared between SVZ and Hippocampus to identify the common genes up and down regulated. In addition, we also identified commonly expressed genes in hippocampus and SVZ at high level....

  2. Expression data from MRTFA-B double knockout brain BioProject

    ID: PRJNA127601

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: -/-;MRTF-BFlox/Flox and three MRTF-A-/-;MRTF-BF/F;GFAP-Cre RNA samples were collected and pooled for hybridization on Affymetrix microarrays....
  3. Upregulated Hedgehog Signaling Promotes Zebrafish CNS Tumorigenesis ArrayExpress

    ID: E-GEOD-40761

    Description: escence studies confirmed overexpression of Pax2, GFAP, S100 and Sox2, specifically in gliomas. We also detected upregulated expression of phosphorylated pRb and Mdm2 associated with gliomagenesis. Our results suggest that dysregulated Hh signaling initiates tumorigenesis in zebrafish CNS. Zebrafish optic nerve maintains phosphorylated pRb expression, therefore provides a unique niche for pathogenesis of glioma. RNAs from 4 gross eye tumors (from fish of 4 months of age) and 4 normal eyes from wil-type control fish were extracted and used for array a...

  4. Cell Image Library Dataset CIL:39751 CIL

    ID: 39751

    Description: taken from the cortex layer 2/3 of an 8 month old GFAP mouse. In this mouse the enhanced green fluorescent protein (EGFP) under the control of the human glial fibrillary acidic protein (GFAP) p...

    Data Types: image

  5. Flow activated cell sorting and genome-wide analysis of hybrids cells (RFP+YFP+) and non-hybrid cells (RFP+) BioProject

    ID: PRJNA310711

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: RFP+ unfused cells using microarrays in the R26Y, GFAP-Cre and FoxA2-Cre models Overall design: HSPCs were unilaterally grafted at day 3 after the last MPTP intraperitoneal injection in mice. One week after transplantation in the midbrain MPTP, cells were dissociated and FACS sorted. We FACS-sorted RFP+YFP+ fused and RFP+ unfused cells (obtained after transplanting HSPCsCre/RFP in MPTP-treated R26Y, HSPCsR26Y in MPTP-treated GFAP-Cre or in MPTP-treated FoxA2-Cre mice) and analyzed their transcriptome profile by microarray analysis...
  6. Expression data of E16 GFAP-tTA: TetO-VEGF-A164 bitransgenic embryos compared to wild type OmicsDI

    ID: E-GEOD-47903

    Date Released: 06-24-2013

    Description: Overexpression of VEGF (vascular endothelial growth factor) in the germinal matrix of the brain causes GMH-IVH-like anomalies (Germinal matrix hemorrh...

  7. Differentially regulted genes in neural stem cells treated by oleanolic acid OmicsDI

    ID: E-GEOD-38394

    Date Released: 06-11-2012

    Description: -specific marker glial fibrillary acidic protein (GFAP) decreased. In immunofluorescence analysis, OA significantly elevated the percentage of TuJ1-positive cells and reduced GFAP-positive cells. Using DNA microarrays, 183 genes were found to be differentially regulated by OA. Neural stem cells derived form mouse embrynic brains were treated with Oleanolic acid or not. Each group had 4 biological relicates each from a mouse....

  8. Cell Image Library Dataset CIL:48105 CIL

    ID: 48105

    Description: erved rat astrocytes were revived and stained for GFAP (in red) to reveal cytoskeleton and DAPI (in blue) to label nuclei. GFAP mutations and intracellular accumulation h...

    Data Types: image

  9. Transcription profiling of mouse conditional knockout of patched (mouse model of medulloblastoma) to investigate the control initiation and progressio... ArrayExpress

    ID: E-GEOD-12430

    Description: loblastomas initiated in neural stem cells (using GFAP-Cre) are more aggressive than those initiated in neural progenitors (using Math1-Cre), and will express more genes associated with self-renewal. In addition, we hypothesize that cancer stem cells (CD15+ cells) from a given tumor will express higher levels of self-renewal genes than non-stem cells (CD15- cells) from the same tumor. Cells from 5 GFAP-Cre tumors, 5 Math1-Cre tumors, and 5 conventional patched-knockout tumors will be isolated using enzymatic digestion and Percoll gradient centrifugation. These procedures have been found to result in 85-95% pure tumor cell populations. In addition, 5 tumors will be FACS-sorted to isolate CD15+ and CD15- populations. RNA will be prepared from each of these samples using an RNeasy kit from Qiagen. Samples of 2 micrograms will be resuspended in 10 microliters of RNase-free water, and sent to the Consortium for labeling, hybridization and analysis. Cells from the three types of tumors, and CD15+ and CD15- cells, will be compared to one another....

  10. Microarray analysis of the astrocyte transcriptome in the ageing brain: relationship to Alzheimer's pathology and ApoE genotype OmicsDI

    ID: E-GEOD-29652

    Date Released: 06-02-2014

    Description: l Cognitive Function and Ageing Study (MRC-CFAS). GFAP-positive astrocytes were isolated from age, sex and brain pH-matched cases derived from the MRC-CFAS cohort, using immuno laser capture microdissection. The extracted RNA was amplified and applied to HGU133 Plus 2.0 Affymetrix gene arrays. Genes were considered differentially expressed if they showed a minimum 1.5 fold change at p<0.02 in all 6 individual cases in each stage of pathology [18 cases in total: 6 cases Braak stages I-II (3 cases carried at least one ApoE epsilon4 allele and 3 were ApoEe4 negative); 6 cases Braak stages III-IV (3 ApoEe4 positive and 3 ApoEe4 negative; 6 cases Braak stages V-VI (3 ApoEe4 positive and 3 ApoEe4 negative)]. keywords: human GFAP-positive astrocytes...

  11. Expression profiling of murine MYCN-driven neuroendocrine tumor from LSL-MYCN;hGFAP-Cre mice ArrayExpress

    ID: E-GEOD-73466

    Description: induction of MYCN in glial fibre acidic protein (GFAP) expressing cells resulting in the development of neuroendocrine tumors of the pancreas and the brain. The expression profiles of nine tumors from pancreas and three brain tumors were compared to non-malignant pancreas controls (GSM771025.CEL-GSM771027.CEL) from wildt...

  12. Expression data of transduced mouse glioblastomas ArrayExpress

    ID: E-GEOD-35917

    Description: Transgenic GFAP-, NES-, and SYN- CRE mice were injected with a lenti-viral construct containing a floxed RFP directly upstream of a cassette c...

  13. Expression data of transduced mouse glioblastomas BioProject

    ID: PRJNA152017

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: Transgenic GFAP-, NES-, and SYN- CRE mice were injected with a lenti-viral construct containing a floxed RFP directly upstream of a cassette c...
  14. Mutant TDP-43 in Astrocytes Kills Motor Neurons in Rats through Neurotoxic Gain and Neuroprotective Loss in Astrocytes BioProject

    ID: PRJNA179143

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: to Doxycline regulation. Astrocytes isolated from GFAP-tTA/TRE-TDP43M337V rats were desiginated as M337V groups and astrocytes isolated from GFAP-tTA single transgenic rats were desiginated as tTA control groups. Total RNA was isolated from cultured astrocytes at varying times (3, 4, or 6 days after Dox withdrawal) after mutant TDP-43 was induced in astrocytes. Upon mutant TDP-43 induction in astroyctes, gene expression profiles in astroyctes were determined by Illumina Direct Hybridization Assay and compared between tTA and M337V groups at the varying time points of mutant TDP-43 induction....
  15. Microarray analysis of the astrocyte transcriptome in the ageing brain: relationship to Alzheimer's pathology and ApoE genotype BioProject

    ID: PRJNA141241

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: tion and Ageing Study (MRC-CFAS). Overall design: GFAP-positive astrocytes were isolated from age, sex and brain pH-matched cases derived from the MRC-CFAS cohort, using immuno laser capture microdissection. The extracted RNA was amplified and applied to HGU133 Plus 2.0 Affymetrix gene arrays. Genes were considered differentially expressed if they showed a minimum 1.5 fold change at p<0.02 in all 6 individual cases in each stage of pathology [18 cases in total: 6 cases Braak stages I-II (3 cases carried at least one ApoE epsilon4 allele and 3 were ApoEe4 negative); 6 cases Braak stages III-IV (3 ApoEe4 positive and 3 ApoEe4 negative; 6 cases Braak stages V-VI (3 ApoEe4 positive and 3 ApoEe4 negative)]. keywords: human GFAP-positive astrocytes...
  16. Gene Expression and DNA methylation profiling in Alzheimer’s Disease patients reveals dysregulated genes associated with site-specific DNA methylati... ArrayExpress

    ID: E-GEOD-84890

    Description: re already associated with AD (i.e. NOTCH1, LRP5, GFAP, and PRKCB); additionally, several genes could represent novel candidates for participation in AD pathophysiology (i.e. MYT1L, RIMBP2, SNRPN and TMEM132D). 97 RNA samples from middle temporal gyrus of Alzheimer's patients (AD); 98 RNA samples from middle temporal gyrus of age and sex matched non demented controls (ND)...

  17. Transcription profiling of human glioma cell lines reveals CD133(+) and CD133(-) glioblastoma-derived cancer stem cells show differential growth chara... ArrayExpress

    ID: E-GEOD-7181

    Description: gnificantly lower proliferation index but similar GFAP staining as compared to CD133+ GBM. Gene arrays from 2x3 representative cells lines are given. Experiment Overall Design: Human glioblastoma cells cultured in DMEM supplemented with EGF, FGF, LIF, B27....

  18. Transcription profiling by array of adult neural stem cells (hGFAP-GFP+/prominin1+ cells) compared to parenchymal astrocytes that are not neurogenic (... ArrayExpress

    ID: E-GEOD-18765

    Description: ult mouse subependymal zone on the basis of their GFAP- and prominin1-expression by fluorescence-activated cell sorting. All self-renewing, multipotent stem cells are contained in this fraction at 70% purity. The stem cell identity of these double-positive cells is further demonstrated in vivo, by using a novel split-Cre-technology for fate mapping. Comparison of the transcriptome of these prospectively isolated neural stem cells to the transcriptomes of other astrocytes or ependymal cells revealed a distinct clustering of the stem cells, highlightin...

  19. CD133+ and CD133- CSC lines in primary human GBM BioProject

    ID: PRJNA98331

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: gnificantly lower proliferation index but similar GFAP staining as compared to CD133+ GBM. Gene arrays from 2x3 representative cells lines are given. Keywords: Cancer stem cell, CD133, glioblastoma Overall design: Human glioblastoma cells cultured in DMEM supplemented with EGF, FGF, LIF, B27....
  20. 11040941 RGD

    Description: SD-company colony (Madison, Wisconsin, USA). The GFAP-TK rat was generated by pronuclear injection of a¿Gfap-Tk' Bacterial Artificial Chromosome (BAC) construct, engineered using ¿RedET¿-mediated homologous recombination methods...

    Creators: National BioResource Project for the Rat in Japan


Displaying 20 of 250 results for "GFAP"