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Displaying 10 of 48 results for "FCGR1A"
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  1. Expression data from Aortic Macrophages ArrayExpress

    ID: E-GEOD-68968

    Description: Aortic macrophages (CD11bhighF4/80highCD45+MerTK+CD64+) were isolated from C57/B6 wild-type male mice aged 6-8 weeks before and 7 days after induction of sepsis by cecal puncture. 18-20 aortas were pooled per sample. Each condition contained three biological replicates....

  2. Gene expression of Th cells, macrophages and monocytes derived from WT and Tbx21-/- Th cell-induced colitis BioProject

    ID: PRJNA271677

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ter, live, single Th cells (CD3+CD4+), monocytes (CD64+Ly-6Chi) and macrophages (CD64+Ly-6Clow) were sorted. Th cells were stimulated for 3 hours in IMDM with 10% FCS containing PMA (10 ng/ml) and ionomycin (1 µg/ml). Total RNA was extracted using the RNeasy Mini kit (Qiagen). The integrity of isolated RNA was assessed for each sample using an Agilent 2100 Bioanalyzer (Agilent, Waldbronn, Germany) and amount was checked with a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). Double-stranded complementary RNA was synthesized from 1 µg total RNA using Message AmpII Biotin (Ambion, USA). Fifteen micrograms of fragmen...
  3. Expression data from intestinal dendritic cells and macrophages of VDTR mice at 4 hours post diphtheria toxin administration BioProject

    ID: PRJNA339056

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ic mice (VDTR) where the primate diphtheria toxin receptor (DTR) fused to enhanced green fluorescent protein (GFP) was driven by the villin promoter enabling expression in intestinal epithelial cells (IEC) of the small and large intestine. We reasoned that intraperitoneal injection of diphtheria toxin would induce apoptosis specifically in IEC, whose sampling could be monitored by tracking the appearance of GFP within professional phagocytes. We chose to explore the transcriptome at 4 hour post diphtheria toxin administration as the number of sampleing phagocytes was maximal at this time point. We found that one dendritic cell subset (herein refered to as CD103) and two macrophage subsets (herein refered to as CD103 CD11b CD64 and CD11b CD64) were responsible for the sampeling of apoptotic IEC. Cells that sampled were refered to as GFPplus and those that did not were GFPminus. Data repres...
  4. Primary human umbilical vein and arterial endothelial cells ArrayExpress

    ID: E-GEOD-1539

    Description: as a negative selection step using CD14, CD45 and CD64 to remove residual contaminating leukocytes, followed by positive selection using a mouse anti-endothelial cell monoclonal antibody (anti-CD146/clone P1H12 purchased from Chemicon, Temecula, CA). Total processing time was limited to 20 to 24 hours. The homogeneous, viable, primary ECs were used immediately to construct the library. The construction of SAGE libraries was performed with the I-SAGE kit (Invitrogen, Carlsbad, CA) per manufacturer’s instructions Keywords: other...

  5. Expression data from Aortic Macrophages BioProject

    ID: PRJNA284232

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: Aortic macrophages (CD11bhighF4/80highCD45+MerTK+CD64+) were isolated from C57/B6 wild-type male mice aged 6-8 weeks before and 7 days after induction of sepsis by cecal puncture. 18-20 aortas were pooled per sample. Each condition contained three biological replicates....
  6. Primary human umbilical vein and arterial endothelial cells BioProject

    ID: PRJNA90863

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: as a negative selection step using CD14, CD45 and CD64 to remove residual contaminating leukocytes, followed by positive selection using a mouse anti-endothelial cell monoclonal antibody (anti-CD146/clone P1H12 purchased from Chemicon, Temecula, CA). Total processing time was limited to 20 to 24 hours. The homogeneous, viable, primary ECs were used immediately to construct the library. The construction of SAGE libraries was performed with the I-SAGE kit (Invitrogen, Carlsbad, CA) per manufacturer’s instructions Keywords: other...
  7. Systems Biology Approach to Study Influenza Vaccine in Children with Autoimmunity (Juvenile Dermatomyositis JDM) 2014/2015 Cohort (see companion studi... ImmPort

    ID: SDY645

    Description: Transcriptional profiling of peripheral blood of pediatric patients with juvenile dermatomyositis (JDM) before/after vaccination with seasonal influen...

    molecularEntity.name: 2014 Dendritic Cell (CD64)
  8. Systems Biology Approach to Study Influenza Vaccine in Children with Autoimmunity (Juvenile Dermatomyositis JDM) 2013/2014 Cohort (see companion studi... ImmPort

    ID: SDY376

    Description: Transcriptional profiling of peripheral blood of pediatric patients with juvenile dermatomyositis (JDM) before/after vaccination with seasonal influen...

    molecularEntity.name: 2013 Dendritic Cell (CD64)

Displaying 10 of 48 results for "FCGR1A"