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Displaying 20 of 320 results for "EWSR1"
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  1. Gene expression profiling of myxoid liposarcomas (validation set INT-B) OmicsDI

    ID: E-GEOD-55465

    Date Released: 10-31-2014

    Description: FUS-CHOP and EWS-CHOP balanced translocations characterize myxoid liposarcoma which encompasses myxoid (ML) and round cell (RC) variants initial...

  2. MicroRNA expression profiling of Ewing sarcoma cancer stem cells OmicsDI

    ID: E-GEOD-30513

    Date Released: 05-04-2014

    Description: d into CD133+ and CD133- fractions. One sample of EWS-FLI1 expressing human pediatric mesenchymal stem cells, divided into CD133+ and CD133- fractions. One sample of STA-ET-8.2 cells, divided into CD133+ and CD133- fractions....

  3. Epigenetic Maintenance of Stemness and Malignancy in Peripheral Neuroectodermal Tumors by EZH2 OmicsDI

    ID: E-GEOD-15890

    Date Released: 05-15-2013

    Description: iating gene silencing. Down-regulation of EZH2 by RNA interference in ET suppressed oncogenic transformation, tumor development and metastasis in a respective mouse model. Further microarray analysis of EZH2 knock down, or functionally linked HDAC-inhibitor treatment revealed an undifferentiated reversible phenotype in ET maintained by EZH2. EZH2 suppression resulted in a generalized loss of H3K27me3 as well as an increase in H3 acety...

  4. Induction of epididymis specific G-protein coupled receptor-64 (GPR64) in Ewing Tumors supports invasiveness and metastatic spread BioProject

    ID: PRJNA129883

    Keywords: Transcriptome or Gene expression

    Access Type: download

  5. Antibody detection of translocations (ADOT) in Ewing Sarcoma ArrayExpress

    ID: E-GEOD-35450

    Description: translocations in poor quality unprocessed total RNA. We demonstrate the feasibility of ADOT by examples in which both known and unknown Ewing sarcoma translocations are identified from cell lines, tumor xenografts, and FFPE primary tumors. These results demonstrate that ADOT may be an effective approach for translocation analysis in clinical specimens with significant RNA degradation and may offer a novel diagnostic tool for translocation-based cancers. We designed oligonucleotide probes for each possible exon-exon combination be...

  6. Oncogene-Mediated Alterations in Chromatin Conformation BioProject

    ID: PRJNA163079

    Keywords: Other

    Access Type: download

    dataset.description: -wide chromosome conformation capture (Hi-C), ERG binding and gene expression, we demonstrate that oncogenic transcription factor over-expression is associated with global, reproducible and functionally coherent changes in chromatin organization. The results presented here have broader implications, as genomic alterations in other cancer types frequently give rise to aberrant transcription factor expression e.g., EWS-FLI1, c-Myc, n-Myc, PML-RARα. Overall design: We used stable isogenic, normal benign prostate epithelial cell lines (RWPE1) that differ with respect to ERG3 over-expression. To test whether ERG over-expression is associated with global changes in chromatin structure, we performed unbiased chromatin interaction mapping using the Hi-C technique from both RWPE1-ERG and RWPE1-GFP cells, with biological replicates. Successful fill-in and ligation were determined as previously reported by testing for a known interac...
  7. Effect of LOX-PP on the gene expression profile of the A673 cell line (Ewing sarcoma) OmicsDI

    ID: E-GEOD-46407

    Date Released: 06-03-2014

    Description: ls and primary tumors and is downregulated by the EWS/FLI1 oncoprotein characteristic of these tumors. Using a doxycycline inducible system to restore LOX expression in an Ewing sarcoma derived cell line, we show that LOX displays tumor suppressor activities. Interestingly, we show that the tumor suppressor activity resides in the propeptide domain of LOX (L...

  8. Induction of epididymis specific G-protein coupled receptor-64 (GPR64) in Ewing Tumors supports invasiveness and metastatic spread OmicsDI

    ID: E-GEOD-23058

    Date Released: 10-12-2011

    Description: helial signature of this tumor and revealed the G-protein coupled receptor-64 (GPR64), an orphan receptor with normal expression restricted to human epididymis, to be highly induced in ET. Down-regulation of GPR64 in ET lines by RNA interference did not reduce their proliferative capacity in vitro as measured by plastic adherence dependent proliferation or contact independent growth in colony forming assays. Of interest inhibition ...

  9. Gene expression analysis of renal cell carcinoma cell line A-498 after 3-hour treatment with englerin A. BioProject

    ID: PRJNA340129

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: his notion. Englerin A has been shown to activate protein kinase C theta (PKCθ) and was proposed to affect cell viability by promoting glucose dependence while simultaneously starving cells of glucose. Englerin A has also been shown to increase cytosolic calcium levels which may play a role in the decreased phosphorylation and activity of the oncoprotein, EWS-FLI1, in Ewing’s sarcoma. Two other groups have reported that englerin A inhibits tumor cell growth by activating the transient receptor potential cation channel, subfamily C, member 4 (TRPC4) ion channel, while a third group reported that englerin A antagonized L-type calcium channels. However, in at least two of these studies, the concentration of englerin A that was required to modulate these calcium channels was much higher than that required to kill renal carcinoma cells and other cells sensitive to the cytotoxic effects of englerin A. For instance, the most recent study reported that englerin A displaced a radiolabeled tool compound bound to an L-type calcium channel in a concentration-dependent manner with a Ki of 5.7 M. In contrast, the EC50 of englerin A in reducing the viability or growth of most renal cancer cell lines in the NCI60 cell panel is less than 60 nM, suggesting that modulation of L-type or TRPC calcium channels is not required for englerin A to induce toxic effects in these cells. It is still not clear what mechanisms account...
  10. Gene expression analysis of renal cell carcinoma cell line A-498 after 20-hour treatment with englerin A. BioProject

    ID: PRJNA340130

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: his notion. Englerin A has been shown to activate protein kinase C theta (PKCθ) and was proposed to affect cell viability by promoting glucose dependence while simultaneously starving cells of glucose. Englerin A has also been shown to increase cytosolic calcium levels which may play a role in the decreased phosphorylation and activity of the oncoprotein, EWS-FLI1, in Ewing’s sarcoma. Two other groups have reported that englerin A inhibits tumor cell growth by activating the transient receptor potential cation channel, subfamily C, member 4 (TRPC4) ion channel, while a third group reported that englerin A antagonized L-type calcium channels. However, in at least two of these studies, the concentration of englerin A that was required to modulate these calcium channels was much higher than that required to kill renal carcinoma cells and other cells sensitive to the cytotoxic effects of englerin A. For instance, the most recent study reported that englerin A displaced a radiolabeled tool compound bound to an L-type calcium channel in a concentration-dependent manner with a Ki of 5.7 M. In contrast, the EC50 of englerin A in reducing the viability or growth of most renal cancer cell lines in the NCI60 cell panel is less than 60 nM, suggesting that modulation of L-type or TRPC calcium channels is not required for englerin A to induce toxic effects in these cells. It is still not clear what mechanisms account...
  11. Gene expression analysis of renal cell carcinoma cell line A-498 after 8-hour treatment with englerin A. BioProject

    ID: PRJNA340131

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: his notion. Englerin A has been shown to activate protein kinase C theta (PKCθ) and was proposed to affect cell viability by promoting glucose dependence while simultaneously starving cells of glucose. Englerin A has also been shown to increase cytosolic calcium levels which may play a role in the decreased phosphorylation and activity of the oncoprotein, EWS-FLI1, in Ewing’s sarcoma. Two other groups have reported that englerin A inhibits tumor cell growth by activating the transient receptor potential cation channel, subfamily C, member 4 (TRPC4) ion channel, while a third group reported that englerin A antagonized L-type calcium channels. However, in at least two of these studies, the concentration of englerin A that was required to modulate these calcium channels was much higher than that required to kill renal carcinoma cells and other cells sensitive to the cytotoxic effects of englerin A. For instance, the most recent study reported that englerin A displaced a radiolabeled tool compound bound to an L-type calcium channel in a concentration-dependent manner with a Ki of 5.7 M. In contrast, the EC50 of englerin A in reducing the viability or growth of most renal cancer cell lines in the NCI60 cell panel is less than 60 nM, suggesting that modulation of L-type or TRPC calcium channels is not required for englerin A to induce toxic effects in these cells. It is still not clear what mechanisms account...
  12. Oncogene-Mediated Alterations in Chromatin Conformation OmicsDI

    ID: E-GEOD-37752

    Date Released: 05-03-2014

    Description: -wide chromosome conformation capture (Hi-C), ERG binding and gene expression, we demonstrate that oncogenic transcription factor over-expression is associated with global, reproducible and functionally coherent changes in chromatin organization. The results presented here have broader implications, as genomic alterations in other cancer types frequently give rise to aberrant transcription factor expression e.g., EWS-FLI1, c-Myc, n-Myc, PML-RARα. We used stable isogenic, normal benign prostate epithelial cell lines (RWPE1) that differ with respect to ERG3 over-expression. To test whether ERG over-expression is associated with global changes in chromatin structure, we performed unbiased chromatin interaction mapping using the Hi-C technique from both RWPE1-ERG and RWPE1-GFP cells, with biological replicates. Successful fill-in and ligation were determined as previously reported by testing for a known interaction between two...

  13. SRP028293 SRA

    Accession: SRX328591

    Organism: Homo sapiens

    Size:

  14. Transcription factor ETS fusion EWS/FLI1 knockdown in Ewing sarcoma cell line: time course GEO

    ID: geo.datasets:GDS4962

    Description: Sarcoma cell line for up to 96hrs after inducible EWSR1/FLI1 knockdown. Oncogenic ETS fusions are driver mutations in diverse cancers, including Ewing sarcoma. Results provide insight into the molecular m...

    Types: Expression profiling by array

    Instrument: GPL571

  15. NOR1 gene and EWS/NOR1 fusion gene overexpression GEO

    ID: geo.datasets:GDS3481

    Description: Analysis of cells overexpressing NOR1 or the EWS/NOR1 fusion gene. NOR1 encodes an orphan nuclear receptor. The EWS gene is involved in various malignancies...

    Types: Expression profiling by array

    Instrument: GPL570

  16. Rhabdomyosarcoma and Ewing's sarcoma comparison GEO

    ID: geo.datasets:GDS971

    Description: s (RMS) to that in 11 pediatric Ewing's sarcomas (EWS). 3 embryonic and 9 alveolar RMS tumors examined. RMS and EWS appear similar in routine histology. Results identify expression profiles that distinguish RMS from

    Types: Expression profiling by array

    Instrument: GPL91

  17. DEEPEST-Fusion CEDAR

    Description: This is a statistical fusion detection algorithm particularly engineered for screening big RNA sequencing databases

  18. Transcription factor FOXO1 silencing effect on Ewing Sarcoma cell line GEO

    ID: geo.datasets:GDS4963

    Description: arcoma cell line A673sh (a doxycycline-inducible, EWS-FLI1-shRNA expressing derivative of A673) transfected with pRS-sh-FOXO1 plasmid targeting FOXO1wt, in the presence or absence of Dox. Results prov...

    Types: Expression profiling by array

    Instrument: GPL570

  19. Ewing family tumor histogenetic origin GEO

    ID: geo.datasets:GDS916

    Description: fected with the Ewing family tumor (EFT)-specific EWS-FLI1 fusion protein to investigate the histogenetic origin of EFTs. The observed EFT-specific gene expression profile supports the concept of a neura...

    Types: Expression profiling by array

    Instrument: GPL96

  20. Cytosine arabinoside effect on Ewing's sarcoma cell line: time course and dose response GEO

    ID: geo.datasets:GDS2733

    Description: dentified as a modulator of the Ewing’s sarcoma EWS/FLI fusion protein. Results provide insight into the specificity of the response of A673 cells to ARA-C....

    Types: Expression profiling by array

    Instrument: GPL4685


Displaying 20 of 320 results for "EWSR1"