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Displaying 20 of 197 results for "ESAM"
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  1. Decorin and EphA2 mediate Carcinoembryonic Antigen Cell Adhesion Molecule 1-L inhibition of colorectal cancer metastasis ArrayExpress

    ID: E-GEOD-73208

    Description: ersus CT cells. Decorin was detected at the peri-endothelial layers of large blood vessels and in liver stellate cells. In metastatic lesions, it was expressed in pericyte-like cells covering capillary endothelium. Phosphokinase differential screening revealed reduced Ephrin type-A receptor 2 (EphA2) expression and activity in CC1-L- versus CT-expressing cells. Treatment of CT cells with recombinant Dcn led to decreased migration and invasion and decreased EphA2-mediated Erk and Akt signaling. CRC patients exhibiting high CC1 and DCN expression, in combination with low EPHA2 expression, benefit from longer 10-year survival than those with high EPHA2 expression. Conclusion CC1-L expression in poorly differentiated CRC inhibits liver metastasis through increased Decorin expression and EphA2-mediated signaling. Quadriplicate samples of each mouse colon cancer cell lines (control cells versus those expressing either short or long isoform of CEACAM1) have been used for RNA extraction...

  2. e expression and function in acute versus chronic endothelial activation... OmicsDI

    ID: E-GEOD-4518

    Date Released: 06-02-2014

    Description: ain insight into mechanisms of prolonged vascular endothelial activation we compared changes in gene expression induced by continuous activation in stable tmTNFalpha expressing cells with changes due to acute TNFalpha challenge in vitro. Affymetrix Genechip® analysis was performed on RNA from control, acute and continuous TNFalp...

  3. Solution NMR structure of CaM bound to the eNOS CaM binding domain peptide PDB

    ID: PDB:2LL7

    Description: Calmodulin, Nitric oxide synthase, endothelial (E.C.1.14.13.39)

  4. Inhibition of VCAM-1 expression by CORM-3: the role of the ubiquitin proteasome system, p38 and mitochondrial respiration BioProject

    ID: PRJNA127685

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ates TNF-alpha mediated inflammatory responses in endothelial cells, yet, the underlying mechanism hereof is still elusive. We sought to explore potential mechanisms by which CO down-regulates VCAM-1 express...
  5. Endothelial Cell Line Expression Patterns BioProject

    ID: PRJNA102843

    Keywords: Transcriptome or Gene expression

    Access Type: download

  6. LSEC: a microenvironment dependent differentiation program in rat including the novel junctional protein Leda-1 OmicsDI

    ID: E-GEOD-20375

    Date Released: 06-10-2011

    Description: hly isolated ex vivo with both lung microvascular endothelial cells (LMEC) and with LSEC cultured for 42h. The LSEC signature consisted of 48 genes both down-regulated in LMEC and in LSEC upon culture (FC>7 in at least one comparison); qRT-PCR confirmation of these genes included numerous family members and signalling pathway-associated molecules. The LSEC differentiation program comprised distinct sets of growth (wnt2, Fzd4, 5, 9, wls, VEGFR1, 2, 3, Nrp2) and transcription factors (Gata4, Lmo3, Tcfec, Maf) as well as endocytosis-related (Stabilin-1/2, Lyve1 and Ehd3) and cytoskeleton-assoc...

  7. Transcription profiling of rat cirrhotic liver treated with angiogenesis inhibitors OmicsDI

    ID: E-GEOD-6929

    Date Released: 03-27-2012

    Description: nt of vascular density and expression of vascular endothelial growth factor-A (VEGF-A), angiopoietin-1, angiopoietin-2 and placental growth factor (PlGF) in cirrhotic livers. The newly formed hepatic vasculature expressed vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). Interestingly, the expression of these adhesion molecules correlated well with local inflammatory infiltrate. Livers of cirrhotic rats treated with angiogenesis inhibitors presented a significant decrease in hepatic vascular density, inflammatory infiltrate, a-SMA abundance, collagen expression and portal pressure. Conclusion: Angiogenesis inhibitors may offer a potential novel therapy for cirrhosis due to its multiple mechanisms of action against angiogenesis, inflammation and fibrosis in cirrhotic livers. Experiment Overall Design: RNA from liver of 4 non-treated cirrhotic rats or 4 rats treated with angiogenesis inhibitors was hyb...

  8. Inflammation-induced chemokine expression in uveal melanoma cell lines stimulates monocyte chemotaxis ArrayExpress

    ID: E-GEOD-55983

    Description: We used an in vitro co-culture system in which UM cell lines and T cells were cultured together, but separated by a membrane. UM gene expression was quantified using a microarray. Protein expression in the supernatant was quantified with ELISA or cytometric bead array. For the monocyte migration assay, a trans-well plate was used. Results: Gene-expression analysis of UM cell lines showed that co-culture with activated T cells resulted in an upregulation of chemokines such as CXCL8, CXCL9, CXCL10, CXCL11, CCL2, CCL5, vascular endothelial growth factor (VEGF), intracellular adhesion

  9. CLL: CD38+CD49d+ vs. CD38-CD49d- BioProject

    ID: PRJNA111739

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ed media from CCL3-stimulated macrophages induced endothelial cells to express VCAM-1, the CD49d ligand, likely through TNFα over-production. These effects were apparent in BMB from CD38+CD49d+CLL, where lymphoid infiltrates were characterized by a prominent meshwork of VCAM-1+ stromal/endothelial cells. Lastly, CD49d engagement by VCAM-1-transfectants increased viability of CD38+CD49d+ CLL cells. Altogether, CD38 and CD49d can be thought of as a part of a consecutive chain of events ultimately leading to improved survival of CLL cells. Overall design: Dual Color reference design with 26 different patients (15 cd38-cd49d- and 11 cd38+cd49d+)...
  10. Mechanical shear effects on flexor tendon cells ArrayExpress

    ID: E-GEOD-4096

    Description: Adhesion formation after flexor tendon repair remains a clinical problem. Early postoperative motion after tendon repair has been d...

  11. L1CAM overexpression in mouse lung endothelial cells (lECs) ArrayExpress

    ID: E-GEOD-45859

    Description: In an attempt to elucidate the molecular mechanisms underlying the multiple roles of L1 in endothelium, we checked whether manipulating its expression...

  12. Transcription profiling of mechanical shear effects on flexor tendon cells ArrayExpress

    ID: E-SMDB-3543

    Description: Adhesion formation after flexor tendon repair remains a clinical problem. Early postoperative motion after tendon repair has been d...

  13. ER alpha rapid signaling is required for estrogen induced proliferation and migration of vascular endothelial cells ArrayExpress

    ID: E-GEOD-72180

    Description: diated by interaction between ERa and the adaptor molecule striatin. Here we identify a triple point mutation (AA 231,233 & 234 KRR->AAA) of full length ERa that blocks its association with striatin and eliminates its ability to perform rapid signaling (without affecting its ability to perform genomic signaling). We have created stably-transfected human vascular endothelial cell lines expressing either WT ERa (WT ECs) or KRR mutant ERa (KRR ECs), and use these cells to show that rapid signaling through ERa is required for the proper regulation of most E2-regulated genes (the data presented in this record), and...

  14. Inflammation-induced chemokine expression in uveal melanoma cell lines stimulates monocyte chemotaxis BioProject

    ID: PRJNA241516

    Keywords: Transcriptome or Gene expression

    Access Type: download

  15. ER alpha rapid signaling is required for estrogen induced proliferation and migration of vascular endothelial cells BioProject

    ID: PRJNA293271

    Keywords: Transcriptome or Gene expression

    Access Type: download

  16. Transcription profiling of mouse T cell model to characterize transendothelial migration across lymphatics ArrayExpress

    ID: E-GEOD-9237

    Description: Sphingosine 1-phosphate (S1P) influences T cell migration into and out of secondary lymphoid organs; however, its mechanism of action remains uncertain. Our previous research...

  17. Sod2 KO anemic mice OmicsDI

    ID: E-GEOD-27053

    Date Released: 11-27-2011

    Description: function and stress response we find a number of cell surface molecules with significant differential expression. Thrombospondin is found at higher levels in SOD2-/- red cell membrane fractions. This is an extracellular matrix glycoprotein involved in cell adhesion and migration that interacts with glycosaminoglycans (especially the heparan sulfate proteoglycan perlecan) expressed at the endothelial cell surface (Feitsma J. Biol. Chem. 2000 275:9396, Vischer Eur. J. Cell. Biol. 1997 73:332, Sun J. Biol. Chem. 1989 15:2885). Thrombospondin is likely an acquired surface molecule on our red cells, which is of interest as this molecule has been found on the surface of sickle red cells and appears to mediate abnormal adhesion of red cells to vessel wall (Hillery Blood 1996 87:4879, Sugihara Blood 1992 15:2634). CD44 is also found at higher levels in SOD2-/- membrane fractions. CD44 is an adhesion receptor for the high molecular weight polysaccharide hyaluronic acid and has been shown recently to be involved in the trafficking of stem cells to the bone marrow (Avigdor Blood January 2004). Higher expression of these molecules on the surface of SOD2-/- red cells may affect t...

  18. Interaction between endothelium and chronic lymphocytic leukemia B-cells rescues from apoptosis and modulates gene expression profile of leukemic cell... ArrayExpress

    ID: E-GEOD-28787

    Description: ells (CLL) reside in close contact with activated endothelial cells (EC) in infiltrated tissues. Here, we investigated the interactions between EC and CLL cells, highlighting molecular networks involved in this ce...

  19. Increased CD226 Expression on CD8+ T cells in Patients with Systemic Sclerosis ArrayExpress

    ID: E-GEOD-63903

    Description: xpression of genes related to immune response and cell adhesion, including CD226 (also known as DNAX accessory molecule-1, DNAM-1), was significantly altered. Moreover, detailed analysis of CD226 revealed that CD226highCD8+ T cells were increased in SSc patients (mean, 50.7%) compared with healthy controls (32.9%) and were appreciably associated with the severity of skin sclerosis and interstitial lung disease. Further, CD226highCD8+ T cells from SSc patients produced abundant IL-13 and were positively correlated with the cytotoxic capacity of CD8+ T cells against HUVECs. Finally, the neutralization of CD226 impaired IL-13 production and cytolysis against HUVECs. These findings indicate that upregulated CD226 expressio...

  20. Expression data from human choroidal melanocyte cultures BioProject

    ID: PRJNA289500

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: -α or with supernatant from activated T-cells (T-cell conditioned media (TCM)). Gene expression analysis was performed using microarrays. Protein levels were quantified with ELISA or cytometric bead array. Supernatants of HCMs were assessed for the capability to attract monocytes in a trans-well plate. Proliferation of activated T-cells was assessed in a direct co-culture with HCMs by a [3H]-thymidine incorporation assay. Results: Stimulation of HCMs with TCM or IFN-γ and TNF-α resulted in increased expression and secretion of CXCL8, CXCL...

Displaying 20 of 197 results for "ESAM"