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Displaying 20 of 129 results for "DDT"
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  1. PACAP dependency of genes regulated in adrenal gland BioProject

    ID: PRJNA97901

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: CTP, 15 mM dTTP and 10 mM aminoallyl dUTP), 0.1 M DDT, and Superscript II reverse transcriptase. The cDNA were labeled with NHS-ester Cy3 or Cy5 dye in the presence of 1 M sodium bicarbonate. Array slides placed in a hybridization chamber (Corning, Corning, NY) were incubated at 42C for 16-24 hr, and successively washed with 0.5X SSC, 0.01% SDS, and 0.06X SSC at room temperature for 10 min each. Arrays were scanned with a GenePix 4000A scanner (Axon, Foster City, CA), and the resulting images were analyzed using IPlab (Fairfax, VA) and a FileMaker Pro 5 (Santa Clara, CA) relational database designed by Zedong Chen, NHGRI. Following background subtraction and normalization, a calculated ratio of Cy3 to Cy5 signal intensities was used to define the relative increase or decrease of a particular transcript. Ratios were calculated only from those spots with a combined ratio quality above 0.3....
  2. Multiple classes of insecticides fail to induce the transcription of known detoxification gene families BioProject

    ID: PRJNA97003

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: induce any P450, GST or esterase gene expression. DDT was the only insecticide tested capable of eliciting an induction response, but only low levels for one GST and one P450. These results are in contrast to the induction responses observed for the natural plant compound caffeine and the barbituate drug phenobarbital, both of which induced a number of P450 and GST genes to high. Our results show that although insects evolve metabolic resistance to insecticides, induction does not usually have a role in survival after insecticide application, and induction studies cannot be used to predict which genes are capable of metabolizing insecticides. This has implications for managing the evolution of metabolic insecticide resistance in natural insect populations. Keywords: induction response after treatment by various compounds Overall design: Drosophila melanogaster larvae were exposed to 7 different compounds and adult males were exposed to 2 different compounds. For each compound 8 replicates of 50 individuals were exposed to the compound and an additional 8 replicates of 50 individuals were exposed to the appropriate control (...
  3. Effects of methoxychlor (MXC) in male largemouth bass BioProject

    ID: PRJNA112715

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: creasingly as an insecticide since the banning of DDT, the primary advantage being that MXC is rapidly metabolized and does not show high levels of bioaccumulation in non-target organisms. However, studies have shown that MXC can be metabolized into additional metabolites, such as mono-demethylated 2-(p-hydroxyphenyl)-2-(p-methoxyphenyl)-1,1,1-trichloroethane (OH-MXC) and bis-demethylated 2,2-bis(p-hydroxyphenyl)-1,1,1-trichloroethane (HPTE), both of which are estrogenic in mammals. In ad...
  4. PACAP dependency of genes regulated in ischemia OmicsDI

    ID: E-GEOD-5902

    Date Released: 05-02-2014

    Description: CTP, 15 mM dTTP and 10 mM aminoallyl dUTP), 0.1 M DDT, and Superscript II reverse transcriptase. The cDNA were labeled with NHS-ester Cy3 or Cy5 dye in the presence of 1 M sodium bicarbonate. Array slides placed in a hybridization chamber (Corning, Corning, NY) were incubated at 42 äaC for 16-24 hr, and successively washed with 0.5X SSC, 0.01% SDS, and 0.06X SSC at room temperature for 10 min each. Arrays were scanned with a GenePix 4000A scanner (Axon, Foster City, CA), and the resulting images were analyzed using IPlab (Fairfax, VA) and a FileMaker Pro 5 (Santa Clara, CA) relational database designed by Zedong Chen, NHGRI. Following background subtraction and normalization, a calculated ratio of Cy3 to Cy5 signal intensities was used to define the relative increase or decrease of a particular transcript. Ratios were calculated only from those spots with a combined ratio quality above 0.3....

  5. PACAP dependency of genes regulated in ischemia ArrayExpress

    ID: E-GEOD-5902

    Description: CTP, 15 mM dTTP and 10 mM aminoallyl dUTP), 0.1 M DDT, and Superscript II reverse transcriptase. The cDNA were labeled with NHS-ester Cy3 or Cy5 dye in the presence of 1 M sodium bicarbonate. Array slides placed in a hybridization chamber (Corning, Corning, NY) were incubated at 42 äaC for 16-24 hr, and successively washed with 0.5X SSC, 0.01% SDS, and 0.06X SSC at room temperature for 10 min each. Arrays were scanned with a GenePix 4000A scanner (Axon, Foster City, CA), and the resulting images were analyzed using IPlab (Fairfax, VA) and a FileMaker Pro 5 (Santa Clara, CA) relational database designed by Zedong Chen, NHGRI. Following background subtraction and normalization, a calculated ratio of Cy3 to Cy5 signal intensities was used to define the relative increase or decrease of a particular transcript. Ratios were calculated only from those spots with a combined ratio quality above 0.3....

  6. Genomic data of the Diamondback moth (Plutella xylostella) GigaDB

    ID: doi:10.5524/100078

    Release Date: 08-21-2014

    Description: ed resistance to dichlorodiphenyltrichloroethane (DDT) in the 1950s and to Bacillus thuringiensis (Bt) toxins in the 1990s and has developed resistance to all classes of insecticide, making it increasingly difficult to control. A strain of the diamondback moth (DBM) (Fuzhou-S), P. xylostella, was reared on radish seedlings without exposure to insecticides for 5 years, spanning at least 100 generations. An inbred line was developed by successive single-pair sibling matings. Male pupae were used for genome sequencing.DNA from the diamondback moth was collected in Fuzhou, China. We sequenced the 0.34 Gb genome to a depth of approximately 131.2 X with short reads from a series of l...

  7. proteomic survey of lung development and nmyc function in the lung BioProject

    ID: PRJNA97713

    Keywords: Other

    Access Type: download

    dataset.description: ucrose, 50 mM Tris-HCl (pH 7.4), 5 mM MgCl2, 1 mM DDT, and 1 mM PMSF using a tight fitting Teflon pestle. The lysate was centrifuged in a bench-top centrifuge at 800 g for 15 minutes; the supernatant served as source for cytosol, mitochondria and microsomes. The pellet, which contains the nuclei was re-homogenized in lysis buffer and centrifuged as above. The nuclei were resuspended in 2 molar sucrose buffer (2M sucrose, 50 mM Tris-HCl (pH 7.4), 5 mM MgCl2, 1 mM DDT, and 1 mM PMSF) and pelleted by ultracentrifugation at 80000 g in a SW40Ti (Beckman) for 35 minutes. Mitochondria were isolated from the crude cytoplasmic fraction by bench-top centrifugation at 6000 g for 15 minutes. The mitochondrial pellet was washed and pelleted twice in lysis buffer. The cytosolic fraction was obtained after removal of the microsomal fraction by ultracentrifugation at 100000 g in a SW60Ti (Beckman) for 1 hour. Organelle protein extraction Nuclear proteins were extracted by resuspending the isolated nuclei in 5 volumes of 20 mM HEPES (pH 7.9), 1.5 mM MgCl2, 0.42 M NaCl, 0.2 mM EDTA, and 25 % glycerol for 30 minutes with gentle shaking at 4ºC. The nuclei were lysed by 10 passages through an 18-gauge needle, and debris was removed by microcentrifugation at 13000 rpm. The supernatant served as nuclear fraction. Mitochondrial proteins were isolated by incubating the mitochondria in a hypotonic lysis buffer [10 mM HEPES, pH 7.9] for 30 minutes on ice, briefly sonicated and debris pelleted at 13000 rpm for 30 minutes. Protein digestion and MudPIT analysis An aliquot of 150 ug of total protein from each fraction was precipitated over night with 5 volumes of ice-cold acetone followed by centrifugation at 13000 rpm for 20 minutes. The protein pellet was solubilized in 8 M urea, 50 mM Tris-HCl (pH 8.5), 1 mM DTT for 1 hour followed by carboxyamidomethylation with 5 mM iodoacetamide for 1 hour at 37ºC. The samples were then diluted to 4 M urea with 100 mM ammonium bicarbonate (pH 8.5) and digested over night with endoproteinase Lys-C at 37ºC. The next day the mixture was further diluted to 2 M urea with 50 mM ammonium bicarbonate (pH 8.5) supplemented with CaCl2 to a final concentration of 1 mM, and incubated over night with Porozyme trypsin beads at 30ºC with rotating. The resulting peptide mixtur...
  8. PACAP dependency of genes regulated in ischemia BioProject

    ID: PRJNA97331

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: CTP, 15 mM dTTP and 10 mM aminoallyl dUTP), 0.1 M DDT, and Superscript II reverse transcriptase. The cDNA were labeled with NHS-ester Cy3 or Cy5 dye in the presence of 1 M sodium bicarbonate. Array slides placed in a hybridization chamber (Corning, Corning, NY) were incubated at 42 äaC for 16-24 hr, and successively washed with 0.5X SSC, 0.01% SDS, and 0.06X SSC at room temperature for 10 min each. Arrays were scanned with a GenePix 4000A scanner (Axon, Foster City, CA), and the resulting images were analyzed using IPlab (Fairfax, VA) and a FileMaker Pro 5 (Santa Clara, CA) relational database designed by Zedong Chen, NHGRI. Following background subtraction and normalization, a calculated ratio of Cy3 to Cy5 signal intensities was used to define the relative increase or decrease of a particular transcript. Ratios were calculated only from those spots with a combined ratio quality above 0.3....
  9. Charleston Heart Study, 1960-2000 ICPSR

    ID: doi:10.3886/ICPSR04050.v3

    Description: The Charleston Heart Study (CHS) represents data collected over a 41-year period (1960-2000) in order to provide an understanding of the natural progr...

    Creators: Nietert, Paul J. Sutherland, Susan E. Bachman, David L. Keil, Julian E. Gazes, Peter Boyle, Edwin

    citation.title: A twenty year follow-up study of cancer mortality and serum levels of DDT

Displaying 20 of 129 results for "DDT"