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  1. Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to adenocarcinomas BioProject

    ID: PRJNA111245

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: factor 3, RHO guanine nucleotide exchange factor 10, RAS guanine releasing protein 2 and 3, and RAS guanine nucleotide-releasing factor 1 that play a role in an activation of the MAP kinases. Notably, we found the tumor suppressors MCC (mutated in colorectal cancers), HEY1 (hairy/enhancer-of-split related with YRPW motif 1), FAT3 (FAT tumor suppressor homolog 3), ARMCX1 (armadillo repeat containing, X-linked 1) and RECK (reversion-inducing-cysteine-rich protein with kazal motifs) to be significantly repressed. Taken collectively, our study provides valuable information for new candidate genes in lung adenocarcinoma induced by exaggerted c-raf kinase activity. Overall design: SP-C/c-raf model SP-C/c-raf transgenic mice were obtained from the laboratory of Prof. Ulf Rapp (University of Würzburg, Germany), who bred the mice in the C57BL/6/DBA/2 hybrid background. We kept the SP-C/c-raf transgenic mice in the C57BL/6 background for at least five generations. Lung cancer samples were derived from SP-C/c-raf mice (aged 12 – 14 months); unaltered lung tissue were always isolated from the transgenic mouse (aged 5 – 7 months). Endogenous normal lung tissue was studied of non-transgenic mice (aged 7 – 10 months). The non-transgenic littermates (wild-type) served as control for transgenic effects. Mice were sacrificed and the lung tissues were immediately frozen on dry ice and stored at -80°C until further analysis. The histopathological diagnosis was based on routinely processed hematoxylin-eosin stains. Microdissection (LMPC – Laser Microbeam Microdissection and Laser Pressure Catapulting) From each frozen lung tissue 10-µm thick sections were prepared and transferred on polyethylene napthalate foil-covered slides (Zeiss, P.A.L.M. Microlaser Technologies GmbH, Bernried, Germany). The sections were fixed in methanol / acetic acid and stained in hematoxylin. The desired cells were microdissected using the PALM MicroLaser systems (Zeiss, P.A.L.M. Microlaser Technologies GmbH, Ber...

Displaying 1 of 1 results for "ARMC10"