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Displaying 20 of 115 results for "THBS1"
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  1. Crystal Structure of the Thrombospondin-1 N-terminal domain PDB

    ID: PDB:1Z78

    Description: Thrombospondin 1

    gene.name: THBS1, TSP, TSP1
  2. ARID-DNA interactions are required for promoter occupancy by SWI/SNF BioProject

    ID: PRJNA147453

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ependent, BAF-A associations at THROMBOSPONDIN 1 (THBS1) led to the concomitant upregulation of this anti-angiogenic protein. Using a THBS1 promoter-reporter gene, we further show that BAF-A directly regulates THBS1 promoter activity in an ARID domain-dependent manner. Our data not only demonstrate that ARID-DNA interactions are physiologically relevant in higher eukaryotes, but also indicate these interactions can facilitate SWI/SNF binding to target sites in vivo. These findings support the model wherein cooperative interactions among intrinsic subunit-chromatin interaction domains and sequence-specific transcription factors drive SWI/SNF recruitment. Overall design: Four-condition experiment, wild-type vs Baf250a/Arid1a^V1068G/V1068G yolk sacs isolated at E8.5 and E9.5. Biological replicates: 3 ...
  3. Gene expression signatures and molecular markers predict response to therapy in locally advanced head and neck squamous cell carcinoma. OmicsDI

    ID: E-GEOD-23036

    Date Released: 06-10-2011

    Description: we confirmed the association of RAB25, DUOX1 and THBS1 expression with LRFS and OS. Patients whose tumors displayed high RAB25 or DUOX1 mRNA levels had longer LRFS and OS than patients with low mRNA levels. In contrast, patients bearing tumors with high THBS1 levels had a shorter OS than patients with low THBS1 levels. RAB25, DUOX1 and THBS1 could be good molecular markers to identify patients who would benefit from genotoxic treatment. Sixty three pretreatment HNSCC biopsies and five normal mucosas were assayed using the Affymetrix HG-U133A2.0 array....

  4. Transcription profiling of human cells stably expressing KSHV-encoded MicroRNAs OmicsDI

    ID: E-GEOD-7554

    Date Released: 06-10-2011

    Description: nd found that protein levels of thrombospondin 1 (THBS1) were decreased >10-fold. THBS1 has previously been reported to be down-regulated in KS lesions and has known activity as a strong tumor suppressor and anti-angiogenic factor, exerting its anti-angiogenic effect in part by activating the latent form of TGF-b. We show that reduced THBS1 expression in the presence of viral miRNAs translates into decreased TGF-b activity. These data suggest that KSHV-encoded miRNAs may contribute directly to pathogenesis by down-regulation of THBS1, a major regulator of cell adhesion, migration, and angiogenesis. Experiment Overall Design: 293 cells were stably transfected with either a vector expressing 10 KHSV encoded microRNAs or empty vector control. Cells were selected through G418. 4 samples were collected for each cell line and RNA collected for Affymetrix microarray analysis. Results show which transcripts are changed in the presence of KSHV microRNA...

  5. Transcription profiling of human cells stably expressing KSHV-encoded MicroRNAs ArrayExpress

    ID: E-GEOD-7554

    Description: nd found that protein levels of thrombospondin 1 (THBS1) were decreased >10-fold. THBS1 has previously been reported to be down-regulated in KS lesions and has known activity as a strong tumor suppressor and anti-angiogenic factor, exerting its anti-angiogenic effect in part by activating the latent form of TGF-b. We show that reduced THBS1 expression in the presence of viral miRNAs translates into decreased TGF-b activity. These data suggest that KSHV-encoded miRNAs may contribute directly to pathogenesis by down-regulation of THBS1, a major regulator of cell adhesion, migration, and angiogenesis. Experiment Overall Design: 293 cells were stably transfected with either a vector expressing 10 KHSV encoded microRNAs or empty vector control. Cells were selected through G418. 4 samples were collected for each cell line and RNA collected for Affymetrix microarray analysis. Results show which transcripts are changed in the presence of KSHV microRNA...

  6. Crystal Structure of the Thrombospondin-1 N-terminal Domain in Complex with Arixtra PDB

    ID: PDB:1ZA4

    Description: Thrombospondin 1

    gene.name: THBS1, TSP, TSP1
  7. Crystal Structure of the Thrombospondin-1 Type 1 Repeats PDB

    ID: PDB:1LSL

    Description: Thrombospondin 1

    gene.name: THBS1
  8. Gene expression signatures and molecular markers predict response to therapy in locally advanced head and neck squamous cell carcinoma ArrayExpress

    ID: E-GEOD-23036

    Description: we confirmed the association of RAB25, DUOX1 and THBS1 expression with LRFS and OS. Patients whose tumors displayed high RAB25 or DUOX1 mRNA levels had longer LRFS and OS than patients with low mRNA levels. In contrast, patients bearing tumors with high THBS1 levels had a shorter OS than patients with low THBS1 levels. RAB25, DUOX1 and THBS1 could be good molecular markers to identify patients who would benefit from genotoxic treatment. Sixty three pretreatment HNSCC biopsies and five normal mucosas were assayed using the Affymetrix HG-U133A2.0 array....

  9. Identification of Cellular Genes Targeted by KSHV-Encoded MicroRNAs BioProject

    ID: PRJNA100371

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: nd found that protein levels of thrombospondin 1 (THBS1) were decreased >10-fold. THBS1 has previously been reported to be down-regulated in KS lesions and has known activity as a strong tumor suppressor and anti-angiogenic factor, exerting its anti-angiogenic effect in part by activating the latent form of TGF-b. We show that reduced THBS1 expression in the presence of viral miRNAs translates into decreased TGF-b activity. These data suggest that KSHV-encoded miRNAs may contribute directly to pathogenesis by down-regulation of THBS1, a major regulator of cell adhesion, migration, and angiogenesis. Keywords: comparison, experiemental versus control Overall design: 293 cells were stably transfected with either a vector expressing 10 KHSV encoded microRNAs or empty vector control. Cells were selected through G418. 4 samples were collected for each cell line and RNA collected for Affymetrix microarray analysis. Results show which transcripts are ...
  10. Tasquinimod, a Quinoline-3-Carboxamide Anti-Angiogenic Agent, treated Prostate Cancer Cell Line in-vivo and in-vitro OmicsDI

    ID: E-GEOD-17031

    Date Released: 06-02-2014

    Description: , cytochrome P450 1A1 (CYP1A1), thrombospondin-1 (THBS1) and Lysyloxidase preprotein (LOX). To further explore the mode of action of ABR-215050, in vitro experiments with gene microarray analysis were performed using LNCaP prostate tumor cells. The array data were validated by real-time PCR (sqRT-PCR), as well as by protein expression experiments. One of the most significant differentially expressed genes after exposure to ABR-215050, was thrombospondin-1 (THBS1). 2-color treatment vs. control microarray experiment comprising four biological replicates. Each biological replicate was hybridized with dye-swap; resulting in eight hybridizations for each dataset (in vitro and in vivo), for a total of 16 hybridizations....

  11. Gene expression profie of OSMF (Oral Submucous Fibrosis) tissues compared with the expression in normal oral tissues ArrayExpress

    ID: E-GEOD-20170

    Description: wn regulated genes also includes RARRES1, TGM2, THBS1, CHGB, MMP3, SPP1 and ALOX12, C4orf7 respectively. Analysis of microarray data revealed TGF-? pathway as a principal gene network involved in the development of OSF. Intriguingly, there was no regulation of CTGF and THBS1 in fibroblasts by arecoline. However, TGF-b1 treatment regulated all these genes in both epithelial and fibroblast cells. Conclusion: We demonstrate over expression of novel genes in OSF and suggest that OSF development involves TGF-b pathway in an epithelial-stromal cooperation. Targeting TGF-b signaling could be an alternate strategy to treat OSF. Two-condition experiment: 10 OSF tissues against 8 pooled normal tissues...

  12. Expression analysis of the glucose deprivation-induced human tumor cell responses ArrayExpress

    ID: E-GEOD-38583

    Description: easing the expression of angiogenesis inhibitors (THBS1, CXCL14 and CXCL10). The markers of Unfolded Protein Response (UPR) (Grp78/Bip, CHOP, ATF4, etc.) were significantly increased. The above results suggest GD may regulate angiogenesis through activation of the UPR. UM-SCC-81B cells (human oral squamous cell carcinoma cell line) were treated with glucose deprivation (GD) for 4 hours (UM-SCC-81B-GD-4) and 24 hours (UM-SCC-81B-GD24). Cells without treatment were used as a non-treatment control (UM-SCC-81B-NT). Each sample was analyzed once, i.e., without biological replicates. The expression of the genes of interest was confirmed with real-time PCR, ELISA and Western blot....

  13. Microarray analysis of gene expression profiles in response to tomato leaves extract treatment in MCF-7 breast cancer cells ArrayExpress

    ID: E-GEOD-33443

    Description: RYAB, PIM1, BTG1, CYR61, HIF1A, CEBP-beta, TXNIP, THBS1, HMG1L1 and HIST2H3D genes. These results demonstrate the potent activity of TLE as an anti-cancer agent. We analyzed untreated (0 hr) and treated MCF-7 breast cancer cells (after 1 hr and 48hrs) using the Affymetrix Human Exon 1.0 ST platform. Chip array was scanned by Affymetrix Microarray Suite/GCOS. Experiment were performed in triplicates. Raw data was processed by GeneSpringGX v7.2 (Agilent Technology, USA)....

  14. CD36_MOUSE UniProt:Swiss-Prot

    ID: Q08857

    Description: ic Required for interaction with thrombospondins, THBS1 and THBS2 Interaction with PTK2, PXN and LYN Critical for TLR4-TLR6 dimerization and signaling S-palmitoyl cysteine S-palmitoyl cysteine S-palmitoyl cysteine S-palmitoyl cysteine N-linked (GlcNAc...) N-linked...

  15. CD36_MESAU UniProt:Swiss-Prot

    ID: P70110

    Description: ic Required for interaction with thrombospondins, THBS1 and THBS2 Interaction with PTK2, PXN and LYN Critical for TLR4-TLR6 dimerization and signaling S-palmitoyl cysteine S-palmitoyl cysteine S-palmitoyl cysteine S-palmitoyl cysteine N-linked (GlcNAc...) N-linked...

  16. DNA Methylation Profiling of Melanoma Cell Lines ArrayExpress

    ID: E-GEOD-28356

    Description: tes. We found transcript levels of UCHL1, COL1A2, THBS1 and TNFRSF10D were inversely correlated with promoter methylation. The effect of this methylation on expression was confirmed at the protein level. Identification of these candidate TSGs and how their silencing is related to melanoma development will increase our understanding of the etiology of this cancer and may provide tools for its early diagnosis. Analysed samples consisted of 11 melanoma cell lines and 1 neonatal foreskin melanocyte pool as a reference. Melanoma cell lines overlap with members of the DNA copy number analysis series GSE9003 and expression profiling series GSE7127 . The matching copy number data GEO sa...

  17. Tasquinimod, a Quinoline-3-Carboxamide Anti-Angiogenic Agent, treated Prostate Cancer Cell Line in-vivo and in-vitro ArrayExpress

    ID: E-GEOD-17031

    Description: , cytochrome P450 1A1 (CYP1A1), thrombospondin-1 (THBS1) and Lysyloxidase preprotein (LOX). To further explore the mode of action of ABR-215050, in vitro experiments with gene microarray analysis were performed using LNCaP prostate tumor cells. The array data were validated by real-time PCR (sqRT-PCR), as well as by protein expression experiments. One of the most significant differentially expressed genes after exposure to ABR-215050, was thrombospondin-1 (THBS1). 2-color treatment vs. control microarray experiment comprising four biological replicates. Each biological replicate was hybridized with dye-swap; resulting in eight hybridizations for each dataset (in vitro and in vivo), for a total of 16 hybridizations....

  18. Epigenetic events in early breast cancer OmicsDI

    ID: E-GEOD-8007

    Date Released: 05-02-2014

    Description: ing Growth Factor Beta (TGFb) signalling pathway (THBS1, TGFb2, TGFb R1 & TGFb R2) were consistently down-regulated in the post-selection HMEC population. Gene suppression was not associated with DNA methylation but was associated with chromatin remodelling, involving a decrease in histone H3 lysine 27 (H3K27) tri-methylation and an increase in histone H3 lysine 9 (H3K9) di-methylation and H3K9 de-acetylation. Similar epigenetic repression was also identified MDAMB453 breast cancer cells and in breast tumour samples. These results demonstrate for the first time that TGFb2, its receptors TGFb R1 & TGFb R2 and activator THBS1 are concordantly suppressed early in breast carcinogenesis by repressive histone modifications and indicate that the TGFb signalling pathway is a novel target for gene activation by epigenetic therapy. Keywords: cell differentiation, breast cancer, gene silencing, epigenetics Two-colour reference design was used. Two biological replicates were included in the study - Bre60 and Bre-80. Each cell line was sampled at pre-selection stage, p...

  19. Gene expression profie of OSMF (Oral Submucous Fibrosis) tissues compared with the expression in normal oral tissues OmicsDI

    ID: E-GEOD-20170

    Date Released: 05-02-2014

    Description: wn regulated genes also includes RARRES1, TGM2, THBS1, CHGB, MMP3, SPP1 and ALOX12, C4orf7 respectively. Analysis of microarray data revealed TGF-? pathway as a principal gene network involved in the development of OSF. Intriguingly, there was no regulation of CTGF and THBS1 in fibroblasts by arecoline. However, TGF-b1 treatment regulated all these genes in both epithelial and fibroblast cells. Conclusion: We demonstrate over expression of novel genes in OSF and suggest that OSF development involves TGF-b pathway in an epithelial-stromal cooperation. Targeting TGF-b signaling could be an alternate strategy to treat OSF. Two-condition experiment: 10 OSF tissues against 8 pooled normal tissues...

  20. Crystal structure of the C. elegans Protein O-fucosyltransferase 2 (CePOFUT2) double mutant (R298K-R299K) in complex with GDP and the human TSR1 from ... PDB

    ID: PDB:5FOE

    Description: GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2, THROMBOSPONDIN-1

    gene.name: THBS1, TSP, TSP1

Displaying 20 of 115 results for "THBS1"