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Displaying 20 of 126 results for "SCX"
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  1. Expression data from embryonic day 15.5 atrioventricular canal regions were isolated from Scx-/- and Scx+/+ mice. BioProject

    ID: PRJNA246425

    Keywords: Transcriptome or Gene expression

    Access Type: download

  2. Expression data from embryonic day 15.5 atrioventricular canal regions were isolated from Scx-/- and Scx+/+ mice ArrayExpress

    ID: E-GEOD-57423

    Description: ose: Our lab has previously shown that Scleraxis (Scx) is require for proper valve development in vivo. In order to fully explore gene networks regulated by Scx durin...

  3. Expression data from embryonic day 15.5 atrioventricular canal regions were isolated from Scx-/- and Scx+/+ mice. OmicsDI

    ID: E-GEOD-57423

    Date Released: 08-14-2014

    Description: ose: Our lab has previously shown that Scleraxis (Scx) is require for proper valve development in vivo. In order to fully explore gene networks regulated by Scx durin...

  4. Hs_Hela-SCX-Ti-IMAC-HILIC_3D_HCD OmicsDI

    ID: PAe003957

    Date Released: 12-31-2013

    Description: Hs_Hela-SCX-Ti-IMAC-HILIC_3D_HCD

  5. Expression data from mouse limb tendon cells during development. BioProject

    ID: PRJNA235887

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: mb tendon cells were isolated based on Scleraxis (Scx) expression at different stages of development: E11.5, E12.5 and E14.5 Microarray comparisons were carried out between tendon progenitor and differentiated stages. Overall design: Forelimbs from E11.5, E12.5 and E14.5 Scx-GFP embryos were coll...
  6. Extending proteome coverage through an on-line 2D SCX-RP ultra-high pressure system OmicsDI

    ID: GPM32320003848

    Date Released:

    Description: e question whether a fast and sensitive online 2D SCX-RP UHPLC-MS/MS workflow, could compete with the current 1D long gradient analysis, making total analysis time- versus proteome coverage and sample used as benchmark parameters. Our new automated 2D-LC/MS system is robust and easy to use, consisting of a homemade SCX column, a trap column and a 50 cm analytical EASY-Spray column. We benchmark the system using small amounts of a human cell lysate digest. The 2D

  7. Expression data from mouse limb tendon cells during development ArrayExpress

    ID: E-GEOD-54207

    Description: mb tendon cells were isolated based on Scleraxis (Scx) expression at different stages of development: E11.5, E12.5 and E14.5 Microarray comparisons were carried out between tendon progenitor and differentiated stages. Forelimbs from E11.5, E12.5 and E14.5 Scx-GFP embryos were collected and dissoc...

  8. Extending proteome coverage through an on-line 2D SCX-RP ultra-high pressure system OmicsDI

    ID: PXD000705

    Date Released: 09-30-2014

    Description: e question whether a fast and sensitive online 2D SCX-RP UHPLC-MS/MS workflow, could compete with the current 1D long gradient analysis, making total analysis time- versus proteome coverage and sample used as benchmark parameters. Our new automated 2D-LC/MS system is robust and easy to use, consisting of a homemade SCX column, a trap column and a 50 cm analytical EASY-Spray column. We benchmark the system using small amounts of a human cell lysate digest. The 2D SCX-RP UHPLC-MS/MS workflow allowed us to identify 56600 unique peptides and 5958 proteins in a total analysis time of just over 8 hours. On the same system a 1D RP UHPLC-MS/MS workflow gave only 22000 peptides and 4200 unique proteins in 6 hours analysis time, increases of 157% and 42% at the peptide and prot...

  9. ERLIC-based strategies for combined quantitative proteome and phosphoproteome analysis with limited sample amounts OmicsDI

    ID: PXD001241

    Date Released: 02-25-2015

    Description: mle sets have been uploaded. 16 fractions ERLIC-1-SCX/RP (2 replicates) for phosphopeptide enrichment, 8 fractions SCX-EL (2 replicates), 12 fractions ERLIC-2 (2 replicates). The following 3 strategies were compared: A 2D strategy -> sample set ERLIC-1-SCX/RP. A pseudo-3Dstrategy -> sample sets ERLIC-1-SCX/RP + SCX-EL and a 3D strategy -> sample sets E...

  10. Highly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic repulsion-hydrophilic interaction chromatography OmicsDI

    ID: PXD001381

    Date Released: 12-02-2014

    Description: bottom-up phosphoproteomics strategy termed ERLIC-SCX/RP-LC-MS. We compared two SPE types (RP, SCX) for an ERLIC run with 7 fractions. The results were used to design a workflow for highly sensitive bottom-up phosphoproteomics (third experiment) combing E...

  11. Expression data from mouse limb tendon cells during development. OmicsDI

    ID: E-GEOD-54207

    Date Released: 06-03-2014

    Description: mb tendon cells were isolated based on Scleraxis (Scx) expression at different stages of development: E11.5, E12.5 and E14.5 Microarray comparisons were carried out between tendon progenitor and differentiated stages. Forelimbs from E11.5, E12.5 and E14.5 Scx-GFP embryos were collected and dissoc...

  12. SCX_CHICK UniProt:Swiss-Prot

    ID: P59101

    Description: Basic helix-loop-helix transcription factor scleraxis bHLH Poly-Gly

    gene.name: SCX
  13. SCX_MOUSE UniProt:Swiss-Prot

    ID: Q64124

    Description: Basic helix-loop-helix transcription factor scleraxis bHLH Poly-Pro

    gene.name: Scx
  14. Targeted SCX based peptide fractionation for optimal sequencing by collision induced, and electron transfer dissociation OmicsDI

    ID: PRD000083

    Date Released: 06-18-2013

    Description: Not available

  15. Macro-porous Reversed Phase Separation of Proteins Combined with Reversed Phase Separation of Phosphopeptides and Tandem Mass Spectrometry for Profili... OmicsDI

    ID: PXD000948

    Date Released: 06-06-2014

    Description: ventional method of using strong cation exchange (SCX)-RPLC separation of peptides combined with MS/MS was also used for analyzing the phosphoproteome. Replicate experiments by the mRP-RPLC method identified 1585 distinct phosphoproteins with 4519 phosphopeptides, compared to 1585 phosphoproteins with 4297 phosphopeptides by SCX-RPLC, with a total of 1947 phosphoproteins and 6278 phosphopeptides identified from the combined results. While the two methods have similar ability in the identification of the phosphoproteome, they produce complementary information. The phosphoproteins identified in this study, including 67 novel phosphorylation sites from 56 breast cancer related proteins, can serve as the entry point for future validation with biological implications in breast cancer....

  16. SCX_MS raw files and RP_MS raw files OmicsDI

    ID: PXD003438

    Date Released: 01-28-2016

    Description: n-line pH gradient-eluted strong cation exchange (SCX)-nano-ESI-MS/MS method was developed for protein identification and tested with mixture of six standard proteins, total lysate of HuH7 and N2a cells, as...

  17. Comparative analysis of OFFGel, SCX with pH gradient, and Reverse Phase at high pH for first dimensional separation of peptides from a membrane enriched protein fr... OmicsDI

    ID: PRD000127

    Date Released: 12-01-2009

    Description: OFFGEL fractions 11 -13(24), LC-MS/MS data.

  18. Proteome analysis by charge state-selective separation of peptides: a multidimensional approach OmicsDI

    ID: PXD000210

    Date Released: 11-27-2013

    Description: tified. The high selectivity displayed during the SCX step (95% to 100%) and the overlaps between proteins identified from the SCX-separated peptide groups, result in additional assets of the procedure that significantly increase the confidence of protein identifications. Raw data files were processed with Mascot Distiller (2.2), the mgf files were searched with Mascot Daemon (2.4), all data was stored in ms_lims Fixed modifications: cysteine alkylation Variable modifications: methionine oxidation, protein N-terminus acetylation, pyroglutamate formation of N-terminal glutamine, cyclic carbamate formation of N-terminal threonine or serine Enzyme: trypsin/P with 2 missed cleavages allowed Precursor mass tolerance: 10 ppm Peptide fragment mass tolerance: 0.5 Da Database: Swiss Prot mouse (version 56.4) The xml files of RH0 - RH1 - RH2 CID were created with pride converter 2.5.5 because this is still able to link to ms_lims. The old converter can n...

  19. CMOP_Ce_MixedFFE0609SCX OmicsDI

    ID: PAe000439

    Date Released:

    Description: FFE 0609 SCX

  20. CMOP_Ce_MixedFFE2010SCX OmicsDI

    ID: PAe000436

    Date Released:

    Description: FFE 2010 SCX


Displaying 20 of 126 results for "SCX"