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Displaying 19 of 19 results for "RNPS1"
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  1. The exon junction complex controls transposable element activity by ensuring the faithful splicing of the piwi transcript BioProject

    ID: PRJNA247816

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: vel function for the EJC and its splicing subunit RnpS1 in preventing transposon accumulation in both Drosophila germline and surrounding follicular cells. This function is mediated specifically through the control of the splicing of the piwi transcript. In absence of RnpS1 one of the piwi intron is retained. This intron contains a weak 5’ splice site as well as degenerate transposon fragments, reminiscent of heterochromatic introns. In addition, we identified a small A/T rich region, which alters its polypyrimidine tract (PPT) and confers the RnpS1’s dependency. Finally, we showed that the removal of this intron by
  2. The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript ArrayExpress

    ID: E-GEOD-59327

    Description: vel function for the EJC and its splicing subunit RnpS1 in preventing transposon accumulation in both Drosophila germline and surrounding somatic follicle cells. This function is mediated specifically through the control of piwi transcript splicing, where in the absence of RnpS1 the fourth intron of piwi is retained. Within this intron the polypyrimidine tract is disrupted by a transposon-adjacent A/T-rich sequence that confers dependence on RnpS1. Finally, we demonstrate that RnpS1-dependent removal of this intron requ...

  3. The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript OmicsDI

    ID: E-GEOD-59327

    Date Released: 12-10-2014

    Description: vel function for the EJC and its splicing subunit RnpS1 in preventing transposon accumulation in both Drosophila germline and surrounding somatic follicle cells. This function is mediated specifically through the control of piwi transcript splicing, where in the absence of RnpS1 the fourth intron of piwi is retained. Within this intron the polypyrimidine tract is disrupted by a transposon-adjacent A/T-rich sequence that confers dependence on RnpS1. Finally, we demonstrate that RnpS1-dependent removal of this intron requ...

  4. The exon junction complex controls transposable element activity by ensuring the faithful splicing of the piwi transcript OmicsDI

    ID: E-GEOD-57710

    Date Released: 12-10-2014

    Description: vel function for the EJC and its splicing subunit RnpS1 in preventing transposon accumulation in both Drosophila germline and surrounding follicular cells. This function is mediated specifically through the control of the splicing of the piwi transcript. In absence of RnpS1 one of the piwi intron is retained. This intron contains a weak 5’ splice site as well as degenerate transposon fragments, reminiscent of heterochromatic introns. In addition, we identified a small A/T rich region, which alters its polypyrimidine tract (PPT) and confers the RnpS1’s dependency. Finally, we showed that the removal of this intron by

  5. The exon junction complex controls transposable element activity by ensuring the faithful splicing of the piwi transcript ArrayExpress

    ID: E-GEOD-57710

    Description: vel function for the EJC and its splicing subunit RnpS1 in preventing transposon accumulation in both Drosophila germline and surrounding follicular cells. This function is mediated specifically through the control of the splicing of the piwi transcript. In absence of RnpS1 one of the piwi intron is retained. This intron contains a weak 5’ splice site as well as degenerate transposon fragments, reminiscent of heterochromatic introns. In addition, we identified a small A/T rich region, which alters its polypyrimidine tract (PPT) and confers the RnpS1’s dependency. Finally, we showed that the removal of this intron by

  6. The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript BioProject

    ID: PRJNA255082

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: vel function for the EJC and its splicing subunit RnpS1 in preventing transposon accumulation in both Drosophila germline and surrounding somatic follicle cells. This function is mediated specifically through the control of piwi transcript splicing, where in the absence of RnpS1 the fourth intron of piwi is retained. Within this intron the polypyrimidine tract is disrupted by a transposon-adjacent A/T-rich sequence that confers dependence on RnpS1. Finally, we demonstrate that RnpS1-dependent removal of this intron requ...
  7. siRNA profiling of human HeLa cells response to UPF1 depletion ArrayExpress

    ID: E-GEOD-7009

    Description: reduced abundance of the exon junction component RNPS1 in one of the HeLa strain analyzed. Furthermore, restoration of functional RNPS1 expression, but not of NMD-inactive mutant proteins, also restores efficient NMD in the RNPS1 deficient cell line. We conclude that cellular concentrations of RNPS1 modify NMD efficiency and propose that the cell type specific co-factor availability represents a novel principle that controls NMD. Experiment Overall Design: HeLa cells were treated with UPF1 siRNA or Luciferase siRNA as a negative control. After 72 hs, cytoplasmic RNA was isolated and the integrity of the RNA was assessed using a Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). We performed preparation, processing, and hybridisation of labelled and fragmented cRNA targets to Affymetrix HG_U133A GeneChipsTM according to the manufacturer’s protocols (Affymetrix Inc., Santa Clara, CA). Oligonucleotide arrays w...

  8. siRNA profiling of human HeLa cells response to UPF1 depletion OmicsDI

    ID: E-GEOD-7009

    Date Released: 05-02-2014

    Description: reduced abundance of the exon junction component RNPS1 in one of the HeLa strain analyzed. Furthermore, restoration of functional RNPS1 expression, but not of NMD-inactive mutant proteins, also restores efficient NMD in the RNPS1 deficient cell line. We conclude that cellular concentrations of RNPS1 modify NMD efficiency and propose that the cell type specific co-factor availability represents a novel principle that controls NMD. Experiment Overall Design: HeLa cells were treated with UPF1 siRNA or Luciferase siRNA as a negative control. After 72 hs, cytoplasmic RNA was isolated and the integrity of the RNA was assessed using a Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). We performed preparation, processing, and hybridisation of labelled and fragmented cRNA targets to Affymetrix HG_U133A GeneChipsTM according to the manufacturer’s protocols (Affymetrix Inc., Santa Clara, CA). Oligonucleotide arrays w...

  9. UPF1-depleted HeLa cells - effect on physiological targets BioProject

    ID: PRJNA99241

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: reduced abundance of the exon junction component RNPS1 in one of the HeLa strain analyzed. Furthermore, restoration of functional RNPS1 expression, but not of NMD-inactive mutant proteins, also restores efficient NMD in the RNPS1 deficient cell line. We conclude that cellular concentrations of RNPS1 modify NMD efficiency and propose that the cell type specific co-factor availability represents a novel principle that controls NMD. Keywords: NMD UPF1 knock down Overall design: HeLa cells were treated with UPF1 siRNA or Luciferase siRNA as a negative control. After 72 hs, cytoplasmic RNA was isolated and the integrity of the RNA was assessed using a Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). We performed preparation, processing, and hybridisation of labelled and fragmented cRNA targets to Affymetrix HG_U133A GeneChipsTM according to the manufacturer’s protocols (Affymetrix Inc., Santa Clara, CA). Oligon...
  10. Analysis of pre-mRNA splicing trans-regulation in human lymphoblastoid cell lines BioProject

    ID: PRJNA230267

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: cing and alternative splicing, we knocked-down 22 RNA-binding proteins previously shown or suspected to be involved in the regulation of splicing (“splicing factors”). We performed knockdown experiments in triplicate in the...
  11. RNP1B_XENLA UniProt:Swiss-Prot

    ID: Q3KPW1

    Description: RNA-binding protein with serine-rich domain

  12. The exon junction complex is required for definition and excision of neighboring introns in Drosophila ArrayExpress

    ID: E-GEOD-58830

    Description: show that the core EJC plus the accessory factors RnpS1 and Acinus aid in definition and efficient splicing of neighboring introns. This requires prior deposition of the EJC in close proximity either from an upstream or downstream splicing event. If present in isolation, EJC-dependent introns are splicing-defective also in wildtype cells. Interestingly, the most affected intron belongs to the piwi locus, which explains the reported transposon de-silencing in EJC-depleted Drosophila ovaries. We propose that the dependency ...

  13. RNP1A_XENLA UniProt:Swiss-Prot

    ID: Q5XG24

    Description: RNA-binding protein with serine-rich domain

  14. Identification of cis- and trans-acting factors involved in the localization of MALAT-1 to nuclear speckles BioProject

    ID: PRJNA125093

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: . The knockdown of the nuclear speckle proteins, RNPS1, SRm160 or IBP160, resulted in the diffusion of MALAT-1 to the nucleoplasm. In addition, we have demonstrated that depletion of MALAT-1 represses the expression of several genes. This repression of gene expression also occurs in response to the delocalization of MALAT-1 from the nuclear speckles. These results suggest that RNPS1, SRm160 and IBP160 contribute to the localization of MALAT-1 to nuclear speckles where it is involved in regulating gene expression Overall design: We used DNA microarray analysis to analyze differentially expressed (up- and down-regulated) genes in cells depleted of MALAT-1 by RNAi. Microarray analysis using the Agilent Whole Human Genome 4 x 44K oligo microarray in two independent MALAT-1 knockdown cell lines transfected with different siRNAs. Total RNA (800 ng) was labeled with either Cy3 or Cy5 dye using an Agi...
  15. RNPS1_RAT UniProt:Swiss-Prot

    ID: Q6AYK1

    Description: RNA-binding protein with serine-rich domain

  16. The exon junction complex is required for definition and excision of neighboring introns in Drosophila BioProject

    ID: PRJNA253623

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: show that the core EJC plus the accessory factors RnpS1 and Acinus aid in definition and efficient splicing of neighboring introns. This requires prior deposition of the EJC in close proximity either from an upstream or downstream splicing event. If present in isolation, EJC-dependent introns are splicing-defective also in wildtype cells. Interestingly, the most affected intron belongs to the piwi locus, which explains the reported transposon de-silencing in EJC-depleted Drosophila ovaries. We propose that the dependency ...
  17. RNPS1_BOVIN UniProt:Swiss-Prot

    ID: A6QR16

    Description: RNA-binding protein with serine-rich domain

  18. RNPS1_PONAB UniProt:Swiss-Prot

    ID: Q5NVM8

    Description: RNA-binding protein with serine-rich domain

  19. The exon junction complex is required for definition and excision of neighboring introns in Drosophila OmicsDI

    ID: E-GEOD-58830

    Date Released: 12-06-2014

    Description: show that the core EJC plus the accessory factors RnpS1 and Acinus aid in definition and efficient splicing of neighboring introns. This requires prior deposition of the EJC in close proximity either from an upstream or downstream splicing event. If present in isolation, EJC-dependent introns are splicing-defective also in wildtype cells. Interestingly, the most affected intron belongs to the piwi locus, which explains the reported transposon de-silencing in EJC-depleted Drosophila ovaries. We propose that the dependency ...


Displaying 19 of 19 results for "RNPS1"