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Displaying 20 of 24 results for "REC114"
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  1. Rec114 ChIP-seq ArrayExpress

    ID: E-GEOD-52970

    Description: time points contains ChIP and input samples) from Rec114-myc ARS+, Rec114-myc ars∆ strains, Rec114-myc tof1∆ARS+ and Rec114-myc tof1∆ ars∆ strains...

  2. Rec114 ChIP-seq BioProject

    ID: PRJNA230576

    Keywords: Epigenomics

    Access Type: download

  3. strand-break (DSB) homeostasis by down-regulating Rec114, an essential component of the DSB-machinery... ArrayExpress

    ID: E-GEOD-43384

    Description: TM/ATR, regulate DSB formation by phosphorylating Rec114, an essential Spo11-accessory protein. Analyses of a non-phosphorylatable- or phosphomimetic- alleles of rec114 revealed that DSB-dependent phosphorylation of Rec114 limited its association with DSB-hotspots resulting in reduction in DSB formation. Also obser...

  4. strand-break (DSB) homeostasis by down-regulating Rec114, an essential component of the DSB-machinery... BioProject

    ID: PRJNA185805

    Keywords: Epigenomics

    Access Type: download

  5. strand-break (DSB) homeostasis by down-regulating Rec114, an essential component of the DSB-machinery... OmicsDI

    ID: E-GEOD-43384

    Date Released: 05-02-2014

    Description: TM/ATR, regulate DSB formation by phosphorylating Rec114, an essential Spo11-accessory protein. Analyses of a non-phosphorylatable- or phosphomimetic- alleles of rec114 revealed that DSB-dependent phosphorylation of Rec114 limited its association with DSB-hotspots resulting in reduction in DSB formation. Also obser...

  6. RE114_MOUSE UniProt:Swiss-Prot

    ID: Q9CWH4

    Description: Meiotic recombination protein REC114 Poly-Ala

  7. Rec114 ChIP-seq OmicsDI

    ID: E-GEOD-52970

    Date Released: 09-06-2014

    Description: time points contains ChIP and input samples) from Rec114-myc ARS+, Rec114-myc ars∆ strains, Rec114-myc tof1∆ARS+ and Rec114-myc tof1∆ ars∆ strains...

  8. RE114_SACPA UniProt:Swiss-Prot

    ID: P78956

    Description: Meiotic recombination protein REC114

  9. RE114_SACPS UniProt:Swiss-Prot

    ID: P78957

    Description: Meiotic recombination protein REC114

  10. RE114_HUMAN UniProt:Swiss-Prot

    ID: Q7Z4M0

    Description: Meiotic recombination protein REC114 In dbSNP:rs12102004.

  11. Spo11-accessory proteins link DNA double-strand break sites to the chromosome axis in early meiotic recombination BioProject

    ID: PRJNA140909

    Keywords: Epigenomics

    Access Type: download

    dataset.description: p in yeast, we show that Spo11-accessory proteins Rec114, Mer2 and Mei4 stably interact with chromosome axis sequences, upon phosphorylation of Mer2 by S-phase Cdk. This axis tethering requires meiotic axis components (Red1/Hop1) and is modulated in a domain-specific fashion by cohesin. Loss of Rec114, Mer2 and Mei4 binding correlates with loss of DSBs. Our results strongly suggest that hotspot sequences become tethered to axis sites by the DSB machinery prior to DSB formation. Overall design: 34 ChIPs...
  12. ChIP-chip of DSB factors in the presence and absence of S phase checkpoint induction ArrayExpress

    ID: E-GEOD-46839

    Description: helicase Mcm2-7 and the meiotic DSB factors Mer2, Rec114 and Mre11. Immunoprecipitated and input DNA samples were differentially labeled and cohybridized to a single microarray. We have included 2-3 biological replicates of each experiment, including one dye swap experiment....

  13. ChIP-chip of DSB factors in the presence and absence of S phase checkpoint induction BioProject

    ID: PRJNA202450

    Keywords: Epigenomics

    Access Type: download

    dataset.description: helicase Mcm2-7 and the meiotic DSB factors Mer2, Rec114 and Mre11. Immunoprecipitated and input DNA samples were differentially labeled and cohybridized to a single microarray. We have included 2-3 biological replicates of each experiment, including one dye swap experiment....
  14. DNA replication and DSB factor binding in the meiotic S-phase checkpoint in budding yeast ArrayExpress

    ID: E-GEOD-46841

    Description: Cdc7 kinase, preclusion of chromosomal loading of Rec114 and Mre11, and lowered abundance of the Spo11 nuclease. Without this checkpoint, cells formed DSBs on partially replicated chromosomes. Importantly, such DSBs frequently failed to be repaired and impeded further DNA synthesis, leading to a rapid loss in cell viability. We conclude that a checkpoint-dependent constraint of DSB formation to duplicated DNA is critical not only for meiotic chromosome assortment, but also to protect genome integrity during gametogenesis. DSB factor association was measured in wild-type and checkpoint mutants strains under non-inducing or replication checkpoint inducing conditions. Additionally, ...

  15. PRJNA202447 BioProject

    Access Type: download

    dataset.description: Cdc7 kinase, preclusion of chromosomal loading of Rec114 and Mre11, and lowered abundance of the Spo11 nuclease. Without this checkpoint, cells formed DSBs on partially replicated chromosomes. Importantly, such DSBs frequently failed to be repaired and impeded further DNA synthesis, leading to a rapid loss in cell viability. We conclude that a checkpoint-dependent constraint of DSB formation to duplicated DNA is critical not only for meiotic chromosome assortment, but also to protect genome integrity during gametogenesis. Overall design: DSB factor association was measured in wild-type and checkpoint mutants strains under non-inducing or replication checkpoint inducing conditions...
  16. ChIP-chip of DSB factors in the presence and absence of S phase checkpoint induction OmicsDI

    ID: E-GEOD-46839

    Date Released: 06-03-2014

    Description: helicase Mcm2-7 and the meiotic DSB factors Mer2, Rec114 and Mre11. Immunoprecipitated and input DNA samples were differentially labeled and cohybridized to a single microarray. We have included 2-3 biological replicates of each experiment, including one dye swap experiment....

  17. DNA replication and DSB factor binding in the meiotic S-phase checkpoint in budding yeast OmicsDI

    ID: E-GEOD-46841

    Date Released: 06-03-2014

    Description: Cdc7 kinase, preclusion of chromosomal loading of Rec114 and Mre11, and lowered abundance of the Spo11 nuclease. Without this checkpoint, cells formed DSBs on partially replicated chromosomes. Importantly, such DSBs frequently failed to be repaired and impeded further DNA synthesis, leading to a rapid loss in cell viability. We conclude that a checkpoint-dependent constraint of DSB formation to duplicated DNA is critical not only for meiotic chromosome assortment, but also to protect genome integrity during gametogenesis. DSB factor association was measured in wild-type and checkpoint mutants strains under non-inducing or replication checkpoint inducing conditions. Additionally, ...

  18. Numerical and Spatial Patterning of Yeast Meiotic DNA Breaks by Tel1 ArrayExpress

    ID: E-GEOD-84696

    Description: ut mutating known Tel1 phosphotargets on Hop1 and Rec114 did not. Deep sequencing of Spo11 oligonucleotides from tel1Δ mutants demonstrated that Tel1 shapes the nonrandom DSB distribution in ways that are distinct but partially overlapping with previously described contributions of the recombination regulator Zip3. Finally, we uncover a context-dependent role for Tel1 in hotspot competition, in which an artificial DSB hotspot inhibits nearby hotspots. Evidence for Tel1-dependent competition involving strong natural hotspots is also provided. Sixteen samples total: The first 12 are two biological replicate Spo11-oligo maps from each of the following: wild type and tel1 each collected at 4, 5, and 6 hours after meiotic induction; the next 4 are one biological replicate Spo11-oligo map from each of the following: wild type and tel1 bearing an artificial hotspot insertion either o...

  19. Numerical and Spatial Patterning of Yeast Meiotic DNA Breaks by Tel1 OmicsDI

    ID: E-GEOD-84696

    Date Released: 07-22-2016

    Description: ut mutating known Tel1 phosphotargets on Hop1 and Rec114 did not. Deep sequencing of Spo11 oligonucleotides from tel1Δ mutants demonstrated that Tel1 shapes the nonrandom DSB distribution in ways that are distinct but partially overlapping with previously described contributions of the recombination regulator Zip3. Finally, we uncover a context-dependent role for Tel1 in hotspot competition, in which an artificial DSB hotspot inhibits nearby hotspots. Evidence for Tel1-dependent competition involving strong natural hotspots is also provided. Sixteen samples total: The first 12 are two biological replicate Spo11-oligo maps from each of the following: wild type and tel1 each collected at 4, 5, and 6 hours after meiotic induction; the next 4 are one biological replicate Spo11-oligo map from each of the following: wild type and tel1 bearing an artificial hotspot insertion either o...

  20. Numerical and Spatial Patterning of Yeast Meiotic DNA Breaks by Tel1 BioProject

    ID: PRJNA330852

    Keywords: Other

    Access Type: download

    dataset.description: ut mutating known Tel1 phosphotargets on Hop1 and Rec114 did not. Deep sequencing of Spo11 oligonucleotides from tel1Δ mutants demonstrated that Tel1 shapes the nonrandom DSB distribution in ways that are distinct but partially overlapping with previously described contributions of the recombination regulator Zip3. Finally, we uncover a context-dependent role for Tel1 in hotspot competition, in which an artificial DSB hotspot inhibits nearby hotspots. Evidence for Tel1-dependent competition involving strong natural hotspots is also provided. Overall design: Eighteen samples total: The first 14 are two biological replicate Spo11-oligo maps from each of the following: wild type and tel1 each collected at 4, 5, and 6 hours after meiotic induction; the next 4 are one biological replicate Spo11-oligo map from each of the following: wild type and tel1 bearing an artificial hotspot i...

Displaying 20 of 24 results for "REC114"