POU3F2 | bioCADDIE Data Discovery Index
Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Displaying 20 of 45 results for "POU3F2"
i
  1. Gene expression changes in response to the overexpression of the Arabidopsis SMB gene, and simultaneous loss of SMB, BRN1 and BRN2 functions BioProject

    ID: PRJNA341940

    Keywords: Transcriptome or Gene expression

    Access Type: download

  2. PO3F2_RAT UniProt:Swiss-Prot

    ID: P56222

    Description: POU domain, class 3, transcription factor 2 POU-specifi...

  3. PO2F3_XENLA UniProt:Swiss-Prot

    ID: P20912

    Description: POU domain, class 2, transcription factor 3 POU-specifi...

  4. PO3F3_MOUSE UniProt:Swiss-Prot

    ID: P31361

    Description: POU domain, class 3, transcription factor 3 POU-specifi...

  5. PO3F3_RAT UniProt:Swiss-Prot

    ID: Q63262

    Description: POU domain, class 3, transcription factor 3 POU-specifi...

  6. PO2F3_MOUSE UniProt:Swiss-Prot

    ID: P31362

    Description: POU domain, class 2, transcription factor 3 POU-specifi...

  7. PO3F1_MOUSE UniProt:Swiss-Prot

    ID: P21952

    Description: POU domain, class 3, transcription factor 1 POU-specific

  8. PO3F1_RAT UniProt:Swiss-Prot

    ID: P20267

    Description: POU domain, class 3, transcription factor 1 POU-specific

  9. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells [ChIP-seq] BioProject

    ID: PRJNA301599

    Keywords: Epigenomics

    Access Type: download

    dataset.description: n, which comprises nPTB, the transcription factor BRN2, and miR-9. These findings suggest that two separate gatekeepers control neuronal conversion and maturation and consecutively overcoming these gatekeepers enables deterministic reprogramming of HAFs into functional neurons. Overall design: Four Brn2 ChIP-seq libraries are analyzed. Brn2 genomic binding positions are revealed in human neural progenitor cells before and after two weeks of differentiation with two specific antibodies....
  10. Optogenetic induction of Brn2 drive neural differentiation BioProject

    ID: PRJNA292417

    Keywords: Transcriptome or Gene expression

    Access Type: download

  11. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells [RNA-seq] BioProject

    ID: PRJNA310707

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: n, which comprises nPTB, the transcription factor BRN2, and miR-9. These findings suggest that two separate gatekeepers control neuronal conversion and maturation and consecutively overcoming these gatekeepers enables deterministic reprogramming of HAFs into functional neurons. Overall design: Six RNA-seq libraries are generated by MAPS approach. Total RNA is extracted from induced neuronal cells derived from control shRNA or PTB shRNA treated human adult fibroblasts. Please note that the 'RNA-seq HAF hygro' sample was on 6 days after switching to N3 media *without* nPTB depletion. The other two 'shPTB 6d' and 'shPTB 3w' were on 6 days and 3 weeks respectively after switching to N3 media *with* nPTB depletion....
  12. Dissecting the direct reprogramming path of fibroblasts into neurons by single cell RNA-sequencing BioProject

    ID: PRJNA279486

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: by the combination of additional factors (Myt1l, Pou3f2). We find transgene silencing and emergence of alternative fates are the major efficiency limits of direct reprogramming. These data provide a high-resolution approach for understanding transcriptome states during lineage differentiation. Overall design: 405 single-cell transcriptomes from 5 time points (0 days, 2 days, 5 days, 20 days and 22 days upon Ascl1 induction) during reprogramming of MEFs into iN cells were analyzed in total; All single cells contain 92 external RNA spike-ins; The following single cell transcriptomes were sequenced: 73 single MEF cells (day 0, iN2), 81 single cells at day 2 (iN1), 55 single cells at day 5 (iN3), 33 single cells at day 20 (iN6) and 73 single cells at day 22 (iN7,iN8) upon Ascl1 induction from virally transfected MEFs. Additionally, we generated the transcriptome of 47 single cells that were induced with Ascl1 for 2 days from a clonal ...
  13. ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs BioProject

    ID: PRJNA152295

    Keywords: Epigenomics

    Access Type: download

  14. A novel way for enriching human melanoma precursor cells to identify cellular targets for antineoplastic therapy ArrayExpress

    ID: E-GEOD-20469

    Description: in addition to enrichment of self-renewing cells [2]. Further characterization of these precursor cultures prepared from 6 human metastatic melanoma lesions revealed increased expression of stemness genes such as nanog and Oct3/4, as well as two recently reported melanoma precursor markers, MDR1 and ABCB5. Consistent with the normal melanoblast cultures we found increased expression of the melanoblast marker POU3F2 (BRN2, DCT). In contrast, the expression of MITF, a melanocyte differentiation marker was reduced by 85% in MPCs when compared with the primary culture (Figure 1a, relative expression of MPC vs. cells obtained from the same patient and cultured in regular conditions). Cell cycle analysis excluded death of differentiated cells as the cause of the precursor enrichment, as no apoptosis was evident (Figure 1b, c). Interestingly, there was an increase in S phase cells, indicating that these cells are undergoing divisions. Similarly, Wang et al. reported that CD133+ glioma cells have higher percentage of S phase cells as compared to CD133- cells [8]. We concluded that these culture conditions cause dedifferentiation of melanocytes in addition to enrichment by increased divisions of melanoma precursor cells. Therefore, we defined these cells as melanoma precursor cells (MPC). To reveal pathways of self-renewal in melanoma we have used two single cell clones that were prepared from primary human melanoma cultures obtained from a primary lesion. These cell clones differed in the self-renewal capacity (in the limiting dilution assay) and in their morphology: high self-renewal/polygonal vs low self-renewal/spindle shaped. We compared the two cell types described above, cultured in either regular RPMI-based media or culture conditions developed to enrich for normal melanocytic precursors (Cook AL, Donatien PD, Smith AG, Murphy M, Jones MK, et al. (2003) Human melanoblasts in culture: expression of BRN2 and synergistic regulation by fibroblast growth factor-2, stem cell factor, and endothelin-3. J Invest Dermatol 121: 1150-1159.), designated MPC (melanoma precursor cells) conditions, for two weeks....

  15. ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs ArrayExpress

    ID: E-GEOD-35496

    Description: We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and

  16. Reconstructing and reprogramming the tumor propagating potential of glioblastoma stem-like cells: RNA-seq ArrayExpress

    ID: E-GEOD-54791

    Description: we identify a core set of neurodevelopmental TFs (POU3F2, SOX2, SALL2, OLIG2) essential for GBM propagation. These TFs coordinately bind and activate TPC-specific regulatory elements, and are sufficient to fully reprogram differentiated GBM cells to ‘induced’ TPCs that recapitulate the epigenetic landscape and phenotype of native TPCs. We reconstruct a TF network model that highlights critical interactions and identifies novel therapeutic targets for eliminating TPCs. Our study establishes the epigenetic basis of a developmental hierarchy in a devastating malignancy, provides detailed insight into the underlying gene regulatory programs, and suggests attendant the...

  17. Reconstructing and reprogramming the tumor propagating potential of glioblastoma stem-like cells: ChIP-seq ArrayExpress

    ID: E-GEOD-54047

    Description: we identify a core set of neurodevelopmental TFs (POU3F2, SOX2, SALL2, OLIG2) essential for GBM propagation. These TFs coordinately bind and activate TPC-specific regulatory elements, and are sufficient to fully reprogram differentiated GBM cells to ‘induced’ TPCs that recapitulate the epigenetic landscape and phenotype of native TPCs. We reconstruct a TF network model that highlights critical interactions and identifies novel therapeutic targets for eliminating TPCs. Our study establishes the epigenetic basis of a developmental hierarchy in a devastating malignancy, provides detailed insight into the underlying gene regulatory programs, and suggests attendant the...

  18. Optogenetic induction of Brn2 drive neural differentiation ArrayExpress

    ID: E-GEOD-71889

    Description: We use optical induction of Brn2 to probe mechanisms for gating embryonic stem cell differentiation mRNA-seq time-course following Brn2 indu...

  19. Optogenetic induction of Brn2 drive neural differentiation OmicsDI

    ID: E-GEOD-71889

    Date Released: 08-29-2015

    Description: We use optical induction of Brn2 to probe mechanisms for gating embryonic stem cell differentiation mRNA-seq time-course following Brn2 indu...

  20. ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs OmicsDI

    ID: E-GEOD-35496

    Date Released: 03-14-2013

    Description: We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and


Displaying 20 of 45 results for "POU3F2"