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Displaying 20 of 28 results for "LPIN1"
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  1. derived from three patients carrying mutations in LPIN1 gene and three matched controls with or without TNF-alpha/IL-1-beta treatment... ArrayExpress

    ID: E-MEXP-3761

    Description: s carrying two recessively-inherited mutations in LPIN1 gene and 3 age and sex matched controls with or without TNF-alpha plus IL-1-beta stimulation for 24h....

  2. lipin 1beta overexpression in mouse liver BioProject

    ID: PRJNA96025

    Keywords: Transcriptome or Gene expression

    Access Type: download

  3. derived from three patients carrying mutations in LPIN1 gene and three matched controls with or without TNF-alpha/IL-1-beta treatment... OmicsDI

    ID: E-MEXP-3761

    Date Released: 10-19-2015

    Description: s carrying two recessively-inherited mutations in LPIN1 gene and 3 age and sex matched controls with or without TNF-alpha plus IL-1-beta stimulation for 24h....

  4. Transcription profiling of mouse liver overexpressing lipin 1beta ArrayExpress

    ID: E-GEOD-5538

    Description: Mutations in the gene encoding lipin 1 cause hepatic steatosis in fld mice, a genetic model of lipodystrophy. Lipin

  5. Expression of Splicing Factor Genes is Reduced in Human Obesity and Contributes to Enhanced Lipogenesis ArrayExpress

    ID: E-GEOD-22435

    Description: nd hypertriglyceridemia. We also demonstrate that LPIN1, a key regulator of lipid metabolism, is a splicing target of SFRS10, with reduced SFRS10 levels favoring the lipogenic β isoform of LPIN1. Importantly, LPIN1β-specific siRNA abolished the lipogenic effects of decreased SFRS10 expression. Together, our results indicate reduced expression of SFRS10 alters LPIN1 splicing and induces lipogenesis, demonstrating that reduced splicing factor expression observed in human tissues may contribute to metabolic phenotypes associated with human obesity. Skeletal muscle samples were obtained from 10 lean control subjects and 7 obese subjects with either IGT or DM2 undergoing elective cholecystectomy. Data for liver samples presented in the same manuscript are available at GEO GSE15653. In this analysis RNA was isolated...

  6. Evaluation of lipin-1 effects on gene expression during TLR4 stimulation of macrophages BioProject

    ID: PRJNA235311

    Keywords: Transcriptome or Gene expression

    Access Type: download

  7. Expression of Splicing Factor Genes is Reduced in Human Obesity and Contributes to Enhanced Lipogenesis BioProject

    ID: PRJNA128545

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: nd hypertriglyceridemia. We also demonstrate that LPIN1, a key regulator of lipid metabolism, is a splicing target of SFRS10, with reduced SFRS10 levels favoring the lipogenic β isoform of LPIN1. Importantly, LPIN1β-specific siRNA abolished the lipogenic effects of decreased SFRS10 expression. Together, our results indicate reduced expression of SFRS10 alters LPIN1 splicing and induces lipogenesis, demonstrating that reduced splicing factor expression observed in human tissues may contribute to metabolic phenotypes associated with human obesity. Overall design: Skeletal muscle samples were obtained from 10 lean control subjects and 7 obese subjects with either IGT or DM2 undergoing elective cholecystectomy. Data for liver samples presented in the same manuscript are available at GEO GSE15653. In this analysis ...
  8. Expression of Splicing Factor Genes is Reduced in Human Obesity and Contributes to Enhanced Lipogenesis OmicsDI

    ID: E-GEOD-22435

    Date Released: 08-15-2011

    Description: nd hypertriglyceridemia. We also demonstrate that LPIN1, a key regulator of lipid metabolism, is a splicing target of SFRS10, with reduced SFRS10 levels favoring the lipogenic β isoform of LPIN1. Importantly, LPIN1β-specific siRNA abolished the lipogenic effects of decreased SFRS10 expression. Together, our results indicate reduced expression of SFRS10 alters LPIN1 splicing and induces lipogenesis, demonstrating that reduced splicing factor expression observed in human tissues may contribute to metabolic phenotypes associated with human obesity. Skeletal muscle samples were obtained from 10 lean control subjects and 7 obese subjects with either IGT or DM2 undergoing elective cholecystectomy. Data for liver samples presented in the same manuscript are available at GEO GSE15653. In this analysis RNA was isolated...

  9. Transcription profiling of mouse liver overexpressing lipin 1beta OmicsDI

    ID: E-GEOD-5538

    Date Released: 03-27-2012

    Description: Mutations in the gene encoding lipin 1 cause hepatic steatosis in fld mice, a genetic model of lipodystrophy. Lipin

  10. Evaluation of lipin-1 effects on gene expression during TLR4 stimulation of macrophages OmicsDI

    ID: E-GEOD-54155

    Date Released: 10-04-2014

    Description: Lipin-1 affects the expression levels of many genes during TLR4 stimulation of macrophages. Gene expression anal...

  11. Aging of myelinating glial cells predominantly affects lipid metabolism and immune response pathways ArrayExpress

    ID: E-MTAB-944

    Description: tants carrying deletions of either Pmp22, SCAP or Lpin1. Seven mice were dissected per developmental or aging time-point. Both sciatic nerves were isolated from each mouse and tissues were pooled per time-point to extract total RNA. For the mutants and their wild-type littermates, sciatic nerves were dissected from three mice per genotype. Tissues were not pooled, which generated triplicates per genotype. Total RNA was extracted, purified and quality-controlled. For each condition, 300 ng of total RNA was used to syn...

  12. Effect of dietary cholesterol on gene expression in the liver of apoE-KO mice OmicsDI

    ID: E-GEOD-27457

    Date Released: 05-03-2014

    Description: ted. To evaluate the impact of high-Chol diet for 1 week on gene expression in the liver of apoE-KO mice, DNA microarray analysis was performed with comparison to apoE-KO mice fed a normal chow. We found that mRNA expression related to lipid metabolism was suppressed by the high-Chol diet in the liver of apoE-KO mice, which includes beta-oxidation and glycerol-3-phosphate (G3P) pathway for TG synthesis. In particular, the mRNA and protein expression of lipin-1 and lipin-2 was markedly decreased. PGC-1α, which up-regulates the transcription of lipin-1, was also suppressed. Lipin is reported to function as a coactivator of PGC-1α and is an inducible amplifier of PPARα, indicating that the suppression of genes involved in beta-oxida...

  13. Evaluation of lipin-1 effects on gene expression during TLR4 stimulation of macrophages ArrayExpress

    ID: E-GEOD-54155

    Description: Lipin-1 affects the expression levels of many genes during TLR4 stimulation of macrophages. Gene expression anal...

  14. Effect of dietary cholesterol on gene expression in the liver of apoE-KO mice BioProject

    ID: PRJNA138469

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ted. To evaluate the impact of high-Chol diet for 1 week on gene expression in the liver of apoE-KO mice, DNA microarray analysis was performed with comparison to apoE-KO mice fed a normal chow. We found that mRNA expression related to lipid metabolism was suppressed by the high-Chol diet in the liver of apoE-KO mice, which includes beta-oxidation and glycerol-3-phosphate (G3P) pathway for TG synthesis. In particular, the mRNA and protein expression of lipin-1 and lipin-2 was markedly decreased. PGC-1α, which up-regulates the transcription of lipin-1, was also suppressed. Lipin is reported to function as a coactivator of PGC-1α and is an inducible amplifier of PPARα, indicating that the suppression of genes involved in beta-oxida...
  15. H. pylori infection blocks DNA damage induced apoptosis by T4SS-dependent upregulation of miR-155 ArrayExpress

    ID: E-GEOD-29388

    Description: ori infection. Thereby we could validate Tspan14, Lpin1, and Pmaip1 as new targets of miR-155 and identified their binding site. These and other already published targets showed a substantial pro-apoptotic potential. We observed that H. pylori infected wild type BMMs were much more resistant to DNA damage induced apoptosis by cisplatin when compared to their respective miR-155-/- BMMs. Our data strongly suggests that there is an additional innate immune mechanism of BMMs leading to the upregulation of the anti-apoptotic miR-155 that causes resistance to DNA damage in BMMs. Microarray experiments were performed as dual-color hybridizations. To compensate for dye-specific effects, an independent dye-reversal color-swap was applied. Bone marrow derived macrophages were 'in vitro' infected with H.pylori P12 and the expression changes were measured....

  16. Profiling of promoter occupancy by PPARα in human hepatoma cells via ChIP-chip analysis ArrayExpress

    ID: E-GEOD-25547

    Description: o SREBP-targets HMGCS1, HMGCR, FDFT1, SC4MOL, and LPIN1, expression of which was induced by GW7647, suggesting cross-talk between PPARα and SREBP signaling. Our data furthermore demonstrate interaction between PPARα and STAT transcription factors in PPARα-mediated transcriptional repression, and suggest interaction between PPARα and TBP and C/EBPα in PPARα-mediated transcriptional activation. Overall, our analysis leads to important new insights into the mechanisms and impact of transcriptional regulation by PPARα in human liver and highlight the importance of cross-talk with other transcription factors. HepG2 cells were grown in phenol red-free Dulbecco’s modified medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 0.1 mg/ml streptomycin and 100 U/ml penicillin. Cells were split the day before experiments. Cells were kept at 37 °C and 5% CO2. The following day, cells were treated with either 100 nM of the PPARα agonist GW7647 or control vehicle (DMSO). Cells used for gene expression analys...

  17. Effect of dietary cholesterol on gene expression in the liver of apoE-KO mice ArrayExpress

    ID: E-GEOD-27457

    Description: ted. To evaluate the impact of high-Chol diet for 1 week on gene expression in the liver of apoE-KO mice, DNA microarray analysis was performed with comparison to apoE-KO mice fed a normal chow. We found that mRNA expression related to lipid metabolism was suppressed by the high-Chol diet in the liver of apoE-KO mice, which includes beta-oxidation and glycerol-3-phosphate (G3P) pathway for TG synthesis. In particular, the mRNA and protein expression of lipin-1 and lipin-2 was markedly decreased. PGC-1α, which up-regulates the transcription of lipin-1, was also suppressed. Lipin is reported to function as a coactivator of PGC-1α and is an inducible amplifier of PPARα, indicating that the suppression of genes involved in beta-oxida...

  18. Aging of myelinating glial cells predominantly affects lipid metabolism and immune response pathways OmicsDI

    ID: E-MTAB-944

    Date Released: 05-02-2014

    Description: tants carrying deletions of either Pmp22, SCAP or Lpin1. Seven mice were dissected per developmental or aging time-point. Both sciatic nerves were isolated from each mouse and tissues were pooled per time-point to extract total RNA. For the mutants and their wild-type littermates, sciatic nerves were dissected from three mice per genotype. Tissues were not pooled, which generated triplicates per genotype. Total RNA was extracted, purified and quality-controlled. For each condition, 300 ng of total RNA was used to syn...

  19. Profiling of promoter occupancy by PPARα in human hepatoma cells via ChIP-chip analysis BioProject

    ID: PRJNA134025

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: o SREBP-targets HMGCS1, HMGCR, FDFT1, SC4MOL, and LPIN1, expression of which was induced by GW7647, suggesting cross-talk between PPARα and SREBP signaling. Our data furthermore demonstrate interaction between PPARα and STAT transcription factors in PPARα-mediated transcriptional repression, and suggest interaction between PPARα and TBP and C/EBPα in PPARα-mediated transcriptional activation. Overall, our analysis leads to important new insights into the mechanisms and impact of transcriptional regulation by PPARα in human liver and highlight the importance of cross-talk with other transcription factors. Overall design: HepG2 cells were grown in phenol red-free Dulbecco’s modified medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 0.1 mg/ml streptomycin and 100 U/ml penicillin. Cells were split the day before experiments. Cells were kept at 37 °C and 5% CO2. The following day, cells were treated with either 100 nM of the PPARα agonist GW7647 or control vehicle (DMSO). Cells used for gene e...
  20. H. pylori infection blocks DNA damage induced apoptosis by T4SS-dependent upregulation of miR-155 BioProject

    ID: PRJNA140035

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ori infection. Thereby we could validate Tspan14, Lpin1, and Pmaip1 as new targets of miR-155 and identified their binding site. These and other already published targets showed a substantial pro-apoptotic potential. We observed that H. pylori infected wild type BMMs were much more resistant to DNA damage induced apoptosis by cisplatin when compared to their respective miR-155-/- BMMs. Our data strongly suggests that there is an additional innate immune mechanism of BMMs leading to the upregulation of the anti-apoptotic miR-155 that causes resistance to DNA damage in BMMs. Overall design: Microarray experiments were performed as dual-color hybridizations. To compensate for dye-specific effects, an independent dye-reversal color-swap was applied. Bone marrow derived macrophages were 'in vitro' infected with H.pylori P12 and the expression changes were measured....

Displaying 20 of 28 results for "LPIN1"