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Displaying 20 of 41 results for "GLOD4"
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  1. Crystal structure of human glyoxalase domain-containing protein 4 (GLOD4) PDB

    ID: PDB:3ZI1

    Description: GLYOXALASE DOMAIN-CONTAINING PROTEIN 4

  2. GLOD4_RAT UniProt:Swiss-Prot

    ID: Q5I0D1

    Description: Glyoxalase domain-containing protein 4 N6-succinyllysine Phosphoserine N6-...

  3. Transcriptional Profile Analysis of RPGRORF15 frameshift mutation ArrayExpress

    ID: E-GEOD-19124

    Description: NKAP). Furthermore, DE genes at 7 weeks (ELOVL6, GLOD4, NDUFS4, and REEP1) and 16 weeks (SLC25A5 and TARS2) are related to mitochondrial functions. Real-time PCR of 11 genes confirmed the microarray results and showed differential expression for additional genes not on the array, such as GFAP, RHO, OPN1SW, CNGB3 and the mutated RPGR. Western blotting and IHC analysis also confirmed the high reliability of the presented transcriptomic data. Conclusions: A list of mutated genes in RPGRORF15 diseased retinas, which are likely candidates to further study their role in age-related photoreceptor degeneration diseases, is reported at different crucial ages. The results indicate that at 7 weeks a combination of non-classical anti- and pro-apoptotic genes appears to be involved in photoreceptor degeneration, whereas at both 7 and 16 weeks expression of mitochondria related genes indicates they may play a relevant role in the disease process. 3 biological replicates each for normal and XLPRA2 affected retinas were analyzed at 7 and 16 weeks of age. Each individual sample was hybridized in a reference design using a custom-made retinal cDNA microarray against brain pool to enable cross comparison between groups. Retina was labeled with Cy5 in all samples but 5615 (GSM474350)....

  4. Transcriptional Profile Analysis of RPGRORF15 frameshift mutation BioProject

    ID: PRJNA120739

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: NKAP). Furthermore, DE genes at 7 weeks (ELOVL6, GLOD4, NDUFS4, and REEP1) and 16 weeks (SLC25A5 and TARS2) are related to mitochondrial functions. Real-time PCR of 11 genes confirmed the microarray results and showed differential expression for additional genes not on the array, such as GFAP, RHO, OPN1SW, CNGB3 and the mutated RPGR. Western blotting and IHC analysis also confirmed the high reliability of the presented transcriptomic data. Conclusions: A list of mutated genes in RPGRORF15 diseased retinas, which are likely candidates to further study their role in age-related photoreceptor degeneration diseases, is reported at different crucial ages. The results indicate that at 7 weeks a combination of non-classical anti- and pro-apoptotic genes appears to be involved in photoreceptor degeneration, whereas at both 7 and 16 weeks expression of mitochondria related genes indicates they may play a relevant role in the disease process. Overall design: 3 biological replicates each for normal and XLPRA2 affected retinas were analyzed at 7 and 16 weeks of age. Each individual sample was hybridized in a reference design using a custom-made retinal cDNA microarray against brain pool to enable cross comparison between groups. Retina was labeled with Cy5 in all samples but 5615 (GSM474350)....
  5. Structure of Human CHMP3 (residues 1-150) PDB

    ID: PDB:3FRV

    Description: Charged multivesicular body protein 3

  6. Transcriptional Profile Analysis of RPGRORF15 frameshift mutation OmicsDI

    ID: E-GEOD-19124

    Date Released: 05-02-2014

    Description: NKAP). Furthermore, DE genes at 7 weeks (ELOVL6, GLOD4, NDUFS4, and REEP1) and 16 weeks (SLC25A5 and TARS2) are related to mitochondrial functions. Real-time PCR of 11 genes confirmed the microarray results and showed differential expression for additional genes not on the array, such as GFAP, RHO, OPN1SW, CNGB3 and the mutated RPGR. Western blotting and IHC analysis also confirmed the high reliability of the presented transcriptomic data. Conclusions: A list of mutated genes in RPGRORF15 diseased retinas, which are likely candidates to further study their role in age-related photoreceptor degeneration diseases, is reported at different crucial ages. The results indicate that at 7 weeks a combination of non-classical anti- and pro-apoptotic genes appears to be involved in photoreceptor degeneration, whereas at both 7 and 16 weeks expression of mitochondria related genes indicates they may play a relevant role in the disease process. 3 biological replicates each for normal and XLPRA2 affected retinas were analyzed at 7 and 16 weeks of age. Each individual sample was hybridized in a reference design using a custom-made retinal cDNA microarray against brain pool to enable cross comparison between groups. Retina was labeled with Cy5 in all samples but 5615 (GSM474350)....

  7. RNA-seq from ENCODE/Stanford/Yale/USC/Harvard OmicsDI

    ID: E-GEOD-35587

    Date Released: 11-06-2012

    Description: A (polyA+) . PCR amplified, adapter ligated cDNA, 150-300nt long, was sequenced on an Illumina GA sequencer. Where designated, cell lines received specific treatments prior to RNA isolation. As indicated, K562 cells were treated with either interferon-a or interferon-g for 30 minutes or 6 hours. These experiments were carried out in conjunction with ChIP-Seq experimen...

  8. Abundant Quantitative Trait Loci for CpG Methylation and Expression Across Human Brain Tissues OmicsDI

    ID: E-GEOD-15745

    Date Released: 12-16-2015

    Description: ess, see http://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000249.v1.p1 Because of our interest in genomic regulation of expression and neurological disorders we embarked upon a series of experiments to provide a brain region-specific contextual framework for genetic and epigenetic regulation of gene expression. We obtained frozen brain tissue from the cerebellum, frontal cortex, pons and temporal cortex from 150 subjects (total 600 tissue samples). We undertook four separate assays across this series; first, genome-wide SNP genotyping; second, assay of >27,000 CpG methylation sites in each of the four brain regions; third, mRNA expression profiling of >22,000 transcripts in all four brain regions; and, fourth, miRNA expression profiling of 735 miRNA transcripts. Here we discuss the results of these experiments, particularly in the context of integrated datasets to define expression and CpG methylation quantitative trait loci (eQTL and methQTL) and detailing differences and similarities across brain regions....

  9. Histone Modifications by ChIP-seq from ENCODE/University of Washington OmicsDI

    ID: E-GEOD-35583

    Date Released: 05-02-2014

    Description: different cell lines (http://hgwdev.cse.ucsc.edu/cgi-bin/hgEncodeVocab?type=cellType) using ChIP-seq high-throughput sequencing. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Cells were grown according to the approved ENCODE cell culture protocols (http://hgwdev.cse.ucsc.edu/ENCODE/protocols/cell). Cells were cross-linked with 1% formaldehyde, and the reaction was quenched by the addition of glycine. Fixed cells were rinsed with PBS, lysed in nuclei lysis buffer,...

  10. Abundant Quantitative Trait Loci Exist for DNA Methylation and Gene Expression in Human Brain dbGaP

    ID: phs000249.v2.p1

    Description: ay based methylation analysis, across a series of 150 subjects from whom we had cerebellum, frontal cortex, pons, and temporal cortex tissue (600 tissue samples). In each tissue we assessed 27,578 DNA methylation sites and the expression level of 22,184 genes. This work was then expanded to include assessment of 232 additional subjects and two tissues (cerebellum and frontal cortex) for whom we collected genotype, RNA expression and DNA methylation data....

    Study Types: Control Set

  11. Abundant Quantitative Trait Loci for CpG Methylation and Expression Across Human Brain Tissues BioProject

    ID: PRJNA116889

    Keywords: Other

    Access Type: download

    dataset.description: ess, see http://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000249.v1.p1 Overall design: Because of our interest in genomic regulation of expression and neurological disorders we embarked upon a series of experiments to provide a brain region-specific contextual framework for genetic and epigenetic regulation of gene expression. We obtained frozen brain tissue from the cerebellum, frontal cortex, pons and temporal cortex from 150 subjects (total 600 tissue samples). We undertook four separate assays across this series; first, genome-wide SNP genotyping; second, assay of >27,000 CpG methylation sites in each of the four brain regions; third, mRNA expression profiling of >22,000 transcripts in all four brain regions; and, fourth, miRNA expression profiling of 735 miRNA transcripts. Here we discuss the results of these experiments, particularly in the context of integrated datasets to define expression and CpG methylation quantitative trait loci (eQTL and methQTL) and detailing differences and similarities across brain regions....
  12. Large-scale temporal gene expression profiling during gonadal differentiation and early gametogenesis in rainbow-trout OmicsDI

    ID: E-GEOD-2151

    Date Released: 03-27-2012

    Description: iction parameters : 21-23 bp length, no more than 4 identical successive nucleotides, 30-70% GC content, a maximum of 2 G or C among the five 3'-end bases, no primer dimers and a short amplicon size (70-150 bp). Secondary structures were searched with DNA mfold (http://bioinfo.math.rpi.edu/~mfold/DNA/form1.cgi). Whenever possible, each pair was chosen with at least one primer flanking an intron-exon boundary, in order to prevent genomic amplification. Real-time RT-PCR Real-time RT-PCR was carried out on an iCycler iQTM (BioRad, Hercules, CA). Reactions were performed in 20µl with 300nM of each primer, 5µl of a 1/50 dilution of the RT reaction and the SYBER-Green PCR master Mix (Eurogentec) according to the manufacturer's instructions. After two incubation steps (50°C 10 min, 95°C 2 min), the thermal cycling protocol was 95°C for 10 min followed by 40 cycles of PCR (95°C 30 s, 60°C or 65°C 1 min). For each primer set the efficiency of the PCR reaction (linear equation : y = slope + intercept) was measured in triplicate on serial dilutions of the same cDNA sample (pool of reverse transcribed RNA samples). Real-time PCR efficiencies for each reaction were then calculated using the formula : Efficiency (E)=[10(1/slope)]-1. Melting curve analysis was also performed for each gene to check the specificity and identity of the RT-PCR products. The relative amount of the target RNA called the Starting Quantity (SQ) was then determined using the I-Cycler IQ software by comparison with the corresponding standard curve for each sample run in duplicate. SQ were calculated as follow : SQ = [10((Ct -intercept)/slope))]-1 where Ct is the Cycle threshold of the unknown sample. Each transcript level was then normalized by division with the expression values of the constitutive elongation factor 1 alpha (EF1a) used as an internal standard....

  13. Whole Genome Association Study of Bipolar Disorder dbGaP

    ID: phs000017.v3.p1

    Description: elated disorders (BARD): 841 cases (691 EA cases, 150 AA cases) This consent group includes a subset of the Bipolar cases. Bipolar disorder only (BDO): 653 cases (388 EA cases, 265 AA cases) This consent group includes a subset of the Bipolar cases....

    Study Types: Case-Control

  14. B and T Cell Determinants of Influenza Vaccine Responses in the Elderly dbGaP

    ID: phs000666.v1.p1

    Description: The study sequenced antibody heavy chain gene rearrangements from human subjects receiving influenza vaccination.

    Study Types: Longitudinal

    study.selectionCriteria: e with the protocol.
  15. Blood pressure > 150 systolic or > 95 diastolic at Visit 1
  16. Chronic Hepatitis B or C
  17. Recent or current use of systemic immunosuppressive medication, including glucocorticoids (corticosteroid nasal sprays and inhaled steroids are permissible). Use of oral steroids (< 20mg predisone - equivalent/day) may be acceptable after review by the investigator.
  18. Autoimmune disease
  19. History of blood dyscrasias or hemoglobinopathies requiring regular medical follow up or hospitalization during the preceding year
  20. Use of anti-coagulation medication such as Coumadin or Lovenox, or anti-platelet agents such as aspirin, Plavix, Aggrenox must be reviewed by investigator to determine if this would affect the volunteer's safety.
  21. Receipt of blood or blood products within the past 6 months
  22. Medical or psychiatric condition or occupational responsibilities that preclude subject compliance with the protocol
  23. Receipt of inactivated vaccine within 14 days prior to vaccination
  24. Receipt of live, attenuated vaccine within 60 days of vaccination
  25. History of Guillain-Barré Syndrome
  26. Pregnant or lactating woman
  27. Use of inv...
  28. Sensitivity to the subjective effects of amphetamine dbGaP

    ID: phs000832.v1.p1

    Description: ntervals (pre-drug administration and 30, 60, 90, 150, and 180 minutes post-drug administration). The subjective measures included in the study were the Profile of Mood States (POMS), Drug Effects Questionnaire (DEQ) and Addiction Research Center Inventory (ARCI). Tasks included were the Digit Symbol Substitution (DSST) and Stop Task....

    Study Types: Cohort

  29. Integrative Clinical Sequencing Analysis of Metastatic Castration Resistant Prostate Cancer Reveals a High Frequency of Clinical Actionability dbGaP

    ID: phs000915.v1.p1

    Description: ained high quality DNA and RNA sequence data from 150 bone or soft tissue biopsies. Central pathology revealed high-grade adenocarcinoma with only four cases (3.6%) showing neuroendocrine differentiation. Aberrations of AR, ETS genes, TP53 and PTEN were frequent (40-60% of cases), with TP53 and AR alterations being the most enriched in mCRPC compared to primary prostate cancer. We identified novel genomic alterations in PIK3CA/B (fusions and mutations); R-spondin, BRAF and RAF1 (fusions); APC (inactivating mutations); delta-catenin (...

  30. Melanoma Genome Sequencing Project dbGaP

    ID: phs000452.v2.p1

    Description: encing tumor and normal DNA from a set of roughly 150 melanomas. For the majority of samples (approximately 90% of the cases) mutations in protein coding genes will be assessed in the exonic DNA of tumor-normal pairs using hybrid capture and paired-end DNA sequencing. Additionally, in a few DNA samples (approximately 10% of the cases) the entire genomes will be analy...

    Study Types: Case Set

  31. Gene Expression in Postmortem DLPFC and Hippocampus from Schizophrenia and Mood Disorders dbGaP

    ID: phs000979.v1.p1

    Description: 79 75 DLPFC (mRNA) 1 2 3 2 347 97 12 15 150 9 2 5 202...

    Study Types: Case-Control

  32. Genetic Measurement of Memory B-cell Recall Using Antibody Repertoire Sequencing dbGaP

    ID: phs000656.v1.p1

    Description: We developed an improved high throughput sequencing approach to measure the quantities and sequences of the repertoire of antibody heavy chain RNA in ...

    Study Types: Longitudinal

    study.selectionCriteria: ce with the protocol.
  33. Blood pressure >150 systolic or >95 diastolic at first study visit.
  34. Hospitalization in the past year for congestive heart failure or emphysema.
  35. Chronic Hepatitis B or C.
  36. Recent or current use of immunosuppressive medication, including systemic glucocorticoids (corticosteroid nasal sprays and topical steroids are permissible; after review by the investigator.
  37. Malignancy, other than squamous cell or basal cell skin cancer (includes solid tumors such as breast cancer or prostate cancer with recurrence in the past year, and any hematologic cancer such as leukemia).
  38. Autoimmune disease (including rheumatoid arthritis treated with immunosuppressive medication such as Plaquenil, methotrexate, prednisone, Enbrel) which, in the opinion of the investigator, might jeopardize volunteer safety or compliance with the protocol.
  39. History of blood dyscrasias, renal disease, or hemoglobinopathies requiring regular medical follow up or hospitalization during the preceding year.
  40. Use of any anti-coagulation medication such as Coumadin or Lovenox, or anti-platelet agents such as aspirin (except up to 325 mg. per day), Plavix, or Aggrenox must be reviewed by investigator to determine if this...
  41. Abundant Quantitative Trait Loci Exist for DNA Methylation and Gene Expression in Human Brain dbGaP

    ID: phs000249.v1.p1

    Description: tal cortex, temporal cortex and pons regions of 150 individuals (600 tissue samples). In each tissue we assessed 27,578 DNA methylation sites and the expression level of 22,184 genes. Our research shows that DNA methylation and RNA expression patterns differ between brain regions. Further we show that DNA genotype is correlated with gene expression and DNA methylation, particularly when the genetic variation is close to the DNA me...

    Study Types: Control Set


Displaying 20 of 41 results for "GLOD4"