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Displaying 20 of 29 results for "GCLM"
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  1. Adaptative changes in protein expression and lysine acetylation induced by decreased glutathione levels in astrocytes OmicsDI

    ID: PXD001834

    Date Released: 10-24-2015

    Description: ned from wild type mice or those deficient in the glutamate-cysteine ligase modifier subunit (GCLM). GCLM knockout astrocytes display an approximately 80% reduction in total glutathione levels. We identified potential molecular targets that are affected by the chronic decrease in glutathione levels and observed an adaptative response mediated by Nrf2 activation. In addition, sequence analysis of peptides more extensively acetylated in the absence of GCLM revealed an enrichment of cysteine residues immediately surrounding the site of acetylation; which suggests a potential specific regulation by decreased glutathione content. Pathway analysis revealed that some pathways exhibited both differential protein expression and lysine acetylation, revealing a coordinated response involving transcriptional and post-translational regulation....

  2. Data from: Decreased brain levels of vitamin B12 in aging, autism and schizophrenia Dryad

    DateIssued: 02-02-2016

    Description: both total Cbl and MeCbl levels were decreased in glutamate-cysteine ligase modulatory subunit knockout (GCLM-KO) mice, which exhibit low GSH levels. Thus our findings reveal a previously unrecognized decrease in brain vitamin B12 status across the lifespan that may reflect an adaptation to increasing antioxidant demand, while accelerated deficits due to GSH deficiency may contribute to neurodevelopmental and neuropsychiatric disorders....

  3. Transcription profiling of UV- or oxidized lipid treated dermal skin fibroblasts ArrayExpress

    ID: E-GEOD-13606

    Description: C and UVA-1 radiation. The cluster included HO-1, glutamate-cysteine ligase modifier subunit (GCLM), aldo-keto reductases-1-C1 and -C2 (AKR1C1, AKR1C2), and interleukin 8 (IL8). These genes are members of the cellular stress response system termed “antioxidant response” or “Phase II detoxification”. Accordingly, the regulatory regions of all these genes contain binding sites for NF-E2-related factor 2 (Nrf2), a major regulator of the antioxidant response. Both UVA-1 irradiation and treatment with oxidized lipids led to increased nuclear accumulation of Nrf2. Silencing expression of Nrf2 using siRNA or using cells and tissue from Nrf2-deficient mice, we show that the induction of the co-regulated genes was suppressed. Expression of other canonical UVA-1-induced genes, including cyclooxygenase 2...

  4. Transcription profiling of Puga- tBHQ and arsenic treated GCLM-WT and KO mice ArrayExpress

    ID: E-MEXP-371

    Description: Evaluate the protective effect of prior tBHQ treatment from arsenic damage at two dosage levels of arsenic (2 and 20 uM), and compare to the effect of...

  5. Crystal structure of Putative gamma-glutamylcysteine synthetase (YP_546622.1) from METHYLOBACILLUS FLAGELLATUS KT at 1.90 A resolution PDB

    ID: PDB:3K1T

    Description: Glutamate--cysteine ligase GshA

  6. Comparison of gene expression profiles of anaplastic glioma with or without the mutated IDH1/2 gene BioProject

    ID: PRJNA230506

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ntative NRF2 target genes, including NQO1, HMOX1, GCLM, TXNRD1, and PRDX1. Overall design: 12 anaplastic gliomas with or without mutated IDH1/2....
  7. UV- or oxidized lipid treated dermal skin fibroblasts BioProject

    ID: PRJNA110627

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: C and UVA-1 radiation. The cluster included HO-1, glutamate-cysteine ligase modifier subunit (GCLM), aldo-keto reductases-1-C1 and -C2 (AKR1C1, AKR1C2), and interleukin 8 (IL8). These genes are members of the cellular stress response system termed “antioxidant response” or “Phase II detoxification”. Accordingly, the regulatory regions of all these genes contain binding sites for NF-E2-related factor 2 (Nrf2), a major regulator of the antioxidant response. Both UVA-1 irradiation and treatment with oxidized lipids led to increased nuclear accumulation of Nrf2. Silencing expression of Nrf2 using siRNA or using cells and tissue from Nrf2-deficient mice, we show that the induction of the co-regulated genes was suppressed. Expression of other canonical UVA-1-induced genes, including cyclooxygenase 2 ...
  8. Crystal Structure of gamma-Glutamylcysteine Synthetase from Escherichia coli B PDB

    ID: PDB:1V4G

    Description: Glutamate--cysteine ligase (E.C.6.3.2.2)

  9. Crystal structure of Gamma-glutamylcysteine synthetase from Escherichia Coli B complexed with Transition-state analogue PDB

    ID: PDB:1VA6

    Description: Glutamate--cysteine ligase (E.C.6.3.2.2)

  10. Comparison of gene expression profiles of anaplastic glioma with or without the mutated IDH1/2 gene ArrayExpress

    ID: E-GEOD-52942

    Description: ntative NRF2 target genes, including NQO1, HMOX1, GCLM, TXNRD1, and PRDX1. 12 anaplastic gliomas with or without mutated IDH1/2....

  11. Crystal Structure of gamma-Glutamylcysteine Synthetase Complexed with Aluminum Fluoride PDB

    ID: PDB:2D33

    Description: Glutamate--cysteine ligase (E.C.6.3.2.2)

  12. Crystal Structure of Michaelis Complex of gamma-Glutamylcysteine Synthetase PDB

    ID: PDB:2D32

    Description: Glutamate--cysteine ligase (E.C.6.3.2.2)

  13. Transcription profiling of UV- or oxidized lipid treated dermal skin fibroblasts OmicsDI

    ID: E-GEOD-13606

    Date Released: 05-01-2014

    Description: C and UVA-1 radiation. The cluster included HO-1, glutamate-cysteine ligase modifier subunit (GCLM), aldo-keto reductases-1-C1 and -C2 (AKR1C1, AKR1C2), and interleukin 8 (IL8). These genes are members of the cellular stress response system termed “antioxidant response” or “Phase II detoxification”. Accordingly, the regulatory regions of all these genes contain binding sites for NF-E2-related factor 2 (Nrf2), a major regulator of the antioxidant response. Both UVA-1 irradiation and treatment with oxidized lipids led to increased nuclear accumulation of Nrf2. Silencing expression of Nrf2 using siRNA or using cells and tissue from Nrf2-deficient mice, we show that the induction of the co-regulated genes was suppressed. Expression of other canonical UVA-1-induced genes, including cyclooxygenase 2...

  14. Transcription profiling of Puga- tBHQ and arsenic treated GCLM-WT and KO mice OmicsDI

    ID: E-MEXP-371

    Date Released: 05-03-2014

    Description: Evaluate the protective effect of prior tBHQ treatment from arsenic damage at two dosage levels of arsenic (2 and 20 uM), and compare to the effect of...

  15. Genome-wide map of BACH1 binding in HEK 293T cells ArrayExpress

    ID: E-GEOD-28051

    Description: , FTH1, ME1, SLC48A1) and redox regulation (GCLC, GCLM, SLC7A11), we also discovered BACH1 target genes effecting cell cycle and apoptosis pathways (ITPR2, CALM1, SQSTM1, TFE3, EWSR1, CDK6, BCL2L11, MAFG) as well as subcellular transport processes (CLSTN1, PSAP, MAPT, vault RNA). The newly identified impact of BACH1 on genes involved in neurodegenerative processes and proliferation provides an interesting basis for future dissection of BACH1-mediated gene repression in neurodegeneration and virus-induced cancerogenesis. Examination of BACH1 binding in HEK 293T cells by chromatin immunoprecipitation-sequencing (CHIP-seq) with input DNA as control....

  16. GSH0_BOVIN UniProt:Swiss-Prot

    ID: Q2T9Y6

    Description: Glutamate--cysteine ligase regulatory subunit Phosphoserine N6-acetyllysin...

  17. Structure of homoglutathione synthetase from Glycine max in open conformation with gamma-glutamyl-cysteine bound. PDB

    ID: PDB:3KAK

    Description: Homoglutathione synthetase (E.C.6.3.2.3)

  18. Comparison of gene expression profiles of anaplastic glioma with or without the mutated IDH1/2 gene OmicsDI

    ID: E-GEOD-52942

    Date Released: 08-25-2015

    Description: ntative NRF2 target genes, including NQO1, HMOX1, GCLM, TXNRD1, and PRDX1. 12 anaplastic gliomas with or without mutated IDH1/2....

  19. Yeast Glutathione Synthase Bound to gamma-glutamyl-cysteine, AMP-PNP and 2 Magnesium Ions PDB

    ID: PDB:1M0W

    Description: glutathione synthetase (E.C.6.3.2.3)

  20. A systems biology-based approach to deciphering the etiology of steatosis employing patient-derived dermal fibroblasts and iPS cells BioProject

    ID: PRJNA162687

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: in the flux through glutathione synthesis as both gamma-glutamylcysteine synthetase and glutathione synthetase have an increased flux. The transcriptomes of dermal fibroblasts derived from two patients with steatosis (60 yr, male BMI 45.7 (H0008) and 33 yr, female, BMI 51.3 (H0007)) and control fibroblasts (19 yr, female, BMI 21, (H0002)) were analyzed. Overall design: Total RNA obtained from dermal fibroblasts derived from two patients with steatosis (60 yr, male BMI 45.7 (H0008) and 33 yr, female, BMI 51.3 (H0007)) and control fibroblasts (19 yr, female, BMI 21, (H0002))....

Displaying 20 of 29 results for "GCLM"