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Displaying 8 of 8 results for "FANCD2"
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  1. FANCD2 Activates Transcription of TAp63 and Suppresses Tumorigenesis ArrayExpress

    ID: E-GEOD-46902

    Description: Fanconi Anemia (FA) is a rare genetic disorder characterized by an increased susceptibility t...

  2. Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine ArrayExpress

    ID: E-GEOD-18598

    Description: gulation. Since the enzymatic activity of LSD1 is FAD (flavin adenine dinucleotide)-dependent, its effects on gene expression may be influenced by FAD availability. To address the importance of histone demethylation in adipogenic differentiation and function, we performed cDNA microarray in LSD1-deficient 3T3-L1 cells as well as in the cells treated with LSD1 inhibitor tranylcypromine (TC). FAD-synthesizing enyme, riboflavin kinase (RFK) -deficient cells were also subjected to the microarray ana...

  3. Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine BioProject

    ID: PRJNA123791

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: gulation. Since the enzymatic activity of LSD1 is FAD (flavin adenine dinucleotide)-dependent, its effects on gene expression may be influenced by FAD availability. To address the importance of histone demethylation in adipogenic differentiation and function, we performed cDNA microarray in LSD1-deficient 3T3-L1 cells as well as in the cells treated with LSD1 inhibitor tranylcypromine (TC). FAD-synthesizing enyme, riboflavin kinase (RFK) -deficient cells were also subjected to the microarray ana...
  4. Exploring the role of sigma factor gene expression on production by Corynebacterium glutamicum: sigma factor H and FMN as example BioProject

    ID: PRJNA287495

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: osphate pathway and for enzymes dependent on FMN, FAD or NADPH as cofactor were upregulated when sigH was overexpressed. To test if sigH overexpression can be exploited for production of riboflavin-derived FMN or FAD, the endogenous gene for bifunctional riboflavin kinase/FMN adenyltransferase was co-expressed with sigH from a plasmid. Balanced expression of sigH and ribF improved accumulation of riboflavin (19.8 ± 0.3 μM) and allowed for its conversion to FMN (33.1 ± 1.8 μM) in the supernatant. While a proof-of-concept was reached, conversion was not complete and titers were not high. This study revealed that inducible and gradable overexpression of sigma factor genes is an interesting approach to switch gene expression profiles and to discover untapped potential of bacteria for chemical production. Overall design: Endogenous sigma factor gene, sigH, was overexpressed in C. glutamicum ATCC13032 from IPTG inducible vector, pEKEx3. Two different concentration of IPTG (10 μM and 15 μM) was used for induction of SigH expression....
  5. Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate BioProject

    ID: PRJNA133817

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: pectrum of purified Dld indicated the presence of FAD as bound cofactor. Inactivation of dld resulted in the loss of the ability to grow with D-lactate, which could be restored by plasmid-borne expression of dld. Heterologous expression of dld from C. glutamicum ATCC 13032 in C. efficiens enabled this species to grow with D-lactate as sole carbon source. Homologs of dld of C. glutamicum ATCC 13032 are not encoded in the sequenced genomes of other corynebacteria and mycobacteria. However, the dld locus of C. glutamicum ATCC 13032 shares 2367 bp of 2372 bp identical nucleotides with the dld locus of Propionibacterium freudenreichii subsp. shermanii, a ...
  6. Discovery and validation of gene classifiers for endocrine disrupting chemicals in Zebrafish (Danio rerio) ArrayExpress

    ID: E-GEOD-38070

    Description: mical abbreviations: EE2, 17α-ethynyl estradiol; FAD, fadrozole; TRB, 17 -trenbolone; FIP, fipronil; PRO, prochloraz; FLU, flutamide; MUS, muscimol; KET, ketoconazole; TRI, trilostane; VIN, vinclozolin Since this study was conducted in several phases, three different version of Agilent zebrafish two color microarrays were used based on their availability at the time. These include G2518A (designID 013223) and G2519F (designID 015064, 019161). There were a total of 58 treatment conditions with various combinations of chemical, tissue type, exposure time, and gender. Each condition contained eight to 12 independent samples, half from chemical-treated fish and half from water- control fish....

  7. Gene expression profiles of familial Alzheimer's disease with presenilin 2 mutation patient-specific induced pluripotent stem cells BioProject

    ID: PRJNA139443

    Keywords: Transcriptome or Gene expression

    Access Type: download

  8. Fathead minnow ovaries exposed in vivo or in vitro to fadrozole BioProject

    ID: PRJNA116933

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ls were analyzed for each of the treatments (24hr FAD in vivo and 12hr FAD in vitro along with corresponding controls). cDNA synthesis, cRNA labeling and amplification and hybridization were performed following the manufacturer’s kits and protocols (Agilent Low RNA Input Fluorescent Linear Amplification Kit and Agilent 60-mer oligo microarray processing protocol; Agilent, Palo Alto, CA). Briefly, a primer containing poly dT and a T7 polymerase promoter was added to 500 ng of total RNA. Reverse transcriptase was added to the reaction to synthesize the first and second strands of cDNA. Next, cRNA was synthesized from the double-stranded cDNA using T7 RNA polymerase, which simultaneously incorporates cyanine 3- (Cy3) or cyanine 5- (Cy5) labeled CTP (Perkin-Elmer, Boston, MA). The ovary samples were labeled with Cy5 while the reference sample was labeled with Cy3. Once the labeling was complete, samples were hybridized to the microarray for 17 hours. The microarrays were washed and scanned with a laser-based detection system (Agilent, Palo Alto, CA)....

Displaying 8 of 8 results for "FANCD2"