DEFB128 | bioCADDIE Data Discovery Index
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Displaying 4 of 4 results for "DEFB128"
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  1. DB128_HYLLA UniProt:Swiss-Prot

    ID: A4H254

    Description: Beta-defensin 128

  2. DB128_PONPY UniProt:Swiss-Prot

    ID: A4H253

    Description: Beta-defensin 128

  3. DB128_MACFA UniProt:Swiss-Prot

    ID: A4H255

    Description: Beta-defensin 128

  4. Expression data from primary bovine mammary epithelial cells (pbMEC) primed with 100 ng/ml LPS and subsequently challenged with heat inactivated E. co... ArrayExpress

    ID: E-GEOD-32186

    Description: Higher expressed were anti-microbial factors (ß-defensin LAP, SLPI), cell and tissue protecting factors (DAF, MUC1, TGM1,-3) as well as mediators of the sentinel function of MEC (CCL5, IL-8). Dampened was the expression of potentially harmful pro-inflammatory master cytokines (IL-1B, IL-6, TNF-alpha and immune effectors (iNOS, matrix metalloproteases). Functional network analysis highlighted the reduced expression of IL-1B and of IRF7 as key to this modulation. Conclusion: LPS-primed MEC are fitter to repel pathogens and better protected against misguided attacks of the immune response. Attenuated is the exuberant expression of factors potentially promoting immunopathological processes. MEC therefore recapitulate many aspects of ET known so far from professional immune cells. Each experiment was performed in triplicate using separate pbMEC cultures (80% confluence) prepared from the udders of udders of three healthy, pregnant (d130 of gestation) cows in the mid of their first lactation. Controls (C.) were cultured for 12 h in GM, washed 3x with PBS and cultivated for additional 18 h (short waiting experiment) or 48 h (long waiting experiment). For the priming experiment (P.) the pbMEC cultures were stimulated (“primed”) at time 0 h with 100 ng/ml LPS , washed three times with PBS and cultivated for another 18 h or 48 h. For the induction trial (I.) cells were challenged with 107/ml particles of heat inactivated E. coli1303 for 6 h. The cultures were handled exactly like the control group (C.) prior to the E. coli challenge. For the induction post priming experiment (I.p.P.) cultures were primed for 12 h with LPS, just as described for the priming group (P.). After 3x washing wi...


Displaying 4 of 4 results for "DEFB128"