CLDN1 | bioCADDIE Data Discovery Index
Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Displaying 20 of 32 results for "CLDN1"
i
  1. Claudin-1 is an Invasion and Metastasis Suppressor of Lung Adenocarcinoma BioProject

    ID: PRJNA108533

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: Background: Claudin-1(CLDN1), one of the key components of tight junction proteins, was found to be down-regulated in human lung adenocarcinomas. This st...
  2. CLD1_MOUSE UniProt:Swiss-Prot

    ID: O88551

    Description: Claudin-1 Cytoplasmic Helical Extracellular Helical Cytoplasmic Helical Extracellular Helical Cytoplasmic Interactions with TJP1, TJP2, TJP3 and PATJ

    gene.name: Cldn1
  3. Transcription profiling of wild type mouse livers and livers from hepatocyte nuclear factor 4alpha deficient mice to study the role of HNF4a in hepato... ArrayExpress

    ID: E-GEOD-3126

    Description: of cellular junctions including tight junctions (Cldn1, Cldn-2, Cldn-12, Ocln, F11r/Jam1, Cxadr, Crb3), adherens junctions (Cdh1), desmosomes (Dsc2, Pkp2, Krt2-8), and gap junctions (Gjb1, Gjb2). Using immunoblotting and immunohistochemistry, we found that the expression of proteins representing each of these junction types was reduced or absent. Analysis of these genes for the presence of putative HNF4a binding sites revealed that 25 of 27 contain such sites. We have used chromatin immunoprecipitation to confirm that HNF4a occupies several of these sites in vivo, including Cdh1, Cldn1, and F11r/Jam1. From these data, we conclude that HNF4_ is a central mediator of the fully differentiated hepatocyte gene expression program and that it orchestrates the expression of cell junction and adhesion proteins required for establishing the hepatic epithelium. Experiment Overall Design: comparison of three hnf4 null livers to three control....

  4. bacteria : bacteria Targeted loci BioProject

    ID: PRJNA383961

    Keywords: targeted loci

    Access Type: download

  5. A Molecular Signature Associated with Human Melanoma Brain Metastasis and Spontaneous Micrometastasis ArrayExpress

    ID: E-GEOD-27980

    Description: , COX-2, MMP1, MMP2 and PRAME and lower levels of CLDN1, CYR61 and IL-6R than the cutaneous variants. These gene products may be involved in melanoma brain metastasis and may serve as novel brain metastasis biomarkers and targets for therapy. 8 Samples (arrays) were analyzed. We generated pairwise comparisons between cutaneous and brain metastatic variants of the same genetic background, using Partek Genomics Suite, in the three melanoma models. Genes with p≤5% and a fold-change difference of ≥1.25 or <-1.25 were selected....

  6. Comparison of HNF4 null mouse embryonic livers with control mouse embryonic livers BioProject

    ID: PRJNA93021

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: of cellular junctions including tight junctions (Cldn1, Cldn-2, Cldn-12, Ocln, F11r/Jam1, Cxadr, Crb3), adherens junctions (Cdh1), desmosomes (Dsc2, Pkp2, Krt2-8), and gap junctions (Gjb1, Gjb2). Using immunoblotting and immunohistochemistry, we found that the expression of proteins representing each of these junction types was reduced or absent. Analysis of these genes for the presence of putative HNF4a binding sites revealed that 25 of 27 contain such sites. We have used chromatin immunoprecipitation to confirm that HNF4a occupies several of these sites in vivo, including Cdh1, Cldn1, and F11r/Jam1. From these data, we conclude that HNF4a is a central mediator of the fully differentiated hepatocyte gene expression program and that it orchestrates the expression of cell junction and adhesion proteins required for establishing the hepatic epithelium. Keywords: E18.5 fetal hnf4 null and control mouse livers Overall design: comparison of three hnf4 null livers to three control...
  7. Downregulation of CK2-Beta is sufficient to drive epithelial-to-mesenchymal transition through Snail 1 induction ArrayExpress

    ID: E-GEOD-28569

    Description: ted, whereas epithelial genes such as CDH1, CDH3, CLDN1, CLDN7, OCLN, KRT5, KRT6B, COL2A1 and MUC1, were inversely turned down. The expression of the EMT-inducing transcription factors, Snail and Zeb, was also significantly up-regulated in accordance with the repression of their known target genes. While showing similar phenotypes, the genetic program of TGFβ1-treated or CK2β-depleted MCF10A cells also exhibited distinct features. These results highlight that in addition to TGFβ1-regulated genes, CK2β-depletion may affect alternative pathways. The microarray analysis was performed with RNAs isolated from parental MCF10A cells and the following MCF10A-derived cells: ΔCK2β- and TGFβ1-treated cells (2ng/ml for 72h) and each from two independent cell cultures (duplicates)....

  8. Smoking-induced Wnt pathway downregulation ArrayExpress

    ID: E-GEOD-19407

    Description: f Wnt target genes, including VEGFA, CCND1, MMP7, CLDN1, SOX9, RHOU (all p<0.05 compared to healthy nonsmokers). As a mechanism to explain this broad, smoking-induced suppression of the Wnt pathway, we assessed expression of the DKK and SFRP families, extracellular regulators that suppress the Wnt pathway. Among these, secreted frizzled-related protein 2 (SFRP2), was up-regulated 4.3-fold (p<0.0001) in healthy smokers and 4.9-fold (p<0.0001) in COPD smokers, an observation confirmed by TaqMan Real-time PCR. AT the protein levels, Western analysis demonstrated SFRP2 up-regulation, and immunohistochemistry demonstrated that the smoking-induced SFRP2 upregulation occurred in differentiated ciliated cells. Finally, cigarette smoke extract mediated up-regulation of SFRP2 and downregulation of Wnt target genes in airway epithelial cells in vitro. These observations are consistent with the hypothesis that the Wnt pathway plays a role in airway epithelial cell differentiation in the adult human airway epithelium, with smoking associated with down-regulation of Wnt pathway, contributing to the dysregulation of airway epithelial differentiation observed in the smoking-related airway disorders. Affymetrix arrays were used to assess gene expression data of genes...

  9. Effect of IL-1β on mesenchymal stem cells ArrayExpress

    ID: E-GEOD-33755

    Description: L-8, IL23A, IL32, Toll-like receptors TLR2, TLR4, CLDN1, metalloproteins MMP1 and MMP3, growth factors CSF2 and TNF, together with adhesion molecules ICAM1 and ICAM4. Functional analysis of MSC proliferation, migration and adhesion to extracellular matrix components revealed that IL-1β did not affect proliferation whereas constitute a throphic factor for MSC and increases adhesion mainly to collagen and laminin. Moreover, IL-1β treatment enhanced the ability of MSC to recruit monocytes and granulocytes cells in vitro. Blockade of NFkβ transcription factor activation with IκB kinase beta (IKKb) siRNA impaired MSC migration, adhesion and leucocyte recruitment, indicating that NF-kB signaling pathway is the main mechanism implicated in these biological processes. These findings are relevant for designing protocols that implicate MSC delivery into inflammatory environments. Comparison between two experimental conditions, ...

  10. Theaflavins enhance intestinal barrier of Caco-2 Cell monolayers through the expression of AMP-activated protein kinase-mediated Occludin, Claudin-1, ... Figshare

    ID: doi:10.6084/M9.FIGSHARE.1291530.V1

    Release Date: 03-10-2016

    Description:

  11. Theaflavins enhance intestinal barrier of Caco-2 Cell monolayers through the expression of AMP-activated protein kinase-mediated Occludin, Claudin-1, ... Figshare

    ID: doi:10.6084/M9.FIGSHARE.1291530

    Release Date: 03-10-2016

    Description:

  12. Transcription profiling of wild type mouse livers and livers from hepatocyte nuclear factor 4alpha deficient mice to study the role of HNF4a in hepato... OmicsDI

    ID: E-GEOD-3126

    Date Released: 03-27-2012

    Description: of cellular junctions including tight junctions (Cldn1, Cldn-2, Cldn-12, Ocln, F11r/Jam1, Cxadr, Crb3), adherens junctions (Cdh1), desmosomes (Dsc2, Pkp2, Krt2-8), and gap junctions (Gjb1, Gjb2). Using immunoblotting and immunohistochemistry, we found that the expression of proteins representing each of these junction types was reduced or absent. Analysis of these genes for the presence of putative HNF4a binding sites revealed that 25 of 27 contain such sites. We have used chromatin immunoprecipitation to confirm that HNF4a occupies several of these sites in vivo, including Cdh1, Cldn1, and F11r/Jam1. From these data, we conclude that HNF4_ is a central mediator of the fully differentiated hepatocyte gene expression program and that it orchestrates the expression of cell junction and adhesion proteins required for establishing the hepatic epithelium. Experiment Overall Design: comparison of three hnf4 null livers to three control....

  13. RNA-sequencing of luminal epithelium and stroma cells isolated by LCM from pseudopregnant day 4 Msx1/Msx2 knockout and control uteri ArrayExpress

    ID: E-GEOD-57680

    Description: We recently demonstrated that Msx genes, which encode muscle segment homeobox (Msh) transcription factors, regulate the transition of the luminal epit...

  14. Smoking-induced Wnt pathway downregulation BioProject

    ID: PRJNA121779

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: f Wnt target genes, including VEGFA, CCND1, MMP7, CLDN1, SOX9, RHOU (all p<0.05 compared to healthy nonsmokers). As a mechanism to explain this broad, smoking-induced suppression of the Wnt pathway, we assessed expression of the DKK and SFRP families, extracellular regulators that suppress the Wnt pathway. Among these, secreted frizzled-related protein 2 (SFRP2), was up-regulated 4.3-fold (p<0.0001) in healthy smokers and 4.9-fold (p<0.0001) in COPD smokers, an observation confirmed by TaqMan Real-time PCR. AT the protein levels, Western analysis demonstrated SFRP2 up-regulation, and immunohistochemistry demonstrated that the smoking-induced SFRP2 upregulation occurred in differentiated ciliated cells. Finally, cigarette smoke extract mediated up-regulation of SFRP2 and downregulation of Wnt target genes in airway epithelial cells in vitro. These observations are consistent with the hypothesis that the Wnt pathway plays a role in airway epithelial cell differentiation in the adult human airway epithelium, with smoking associated with down-regulation of Wnt pathway, contributing to the dysregulation of airway epithelial differentiation observed in the smoking-related airway disorders. Overall design: Affymetrix arrays were used to assess gene expressi...
  15. Expression data for human epithelium from subjects with atopic dermatitis, psoriasis and nonatopic controls ArrayExpress

    ID: E-GEOD-26952

    Description: The expression and function of tight junction genes, in particular claudin-1, was studied in order to further understanding of the biology underlying ...

  16. Expression data for human epithelium from subjects with atopic dermatitis, psoriasis and nonatopic controls BioProject

    ID: PRJNA136959

    Keywords: Transcriptome or Gene expression

    Access Type: download

  17. Effect of IL-1β on mesenchymal stem cells BioProject

    ID: PRJNA148405

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: L-8, IL23A, IL32, Toll-like receptors TLR2, TLR4, CLDN1, metalloproteins MMP1 and MMP3, growth factors CSF2 and TNF, together with adhesion molecules ICAM1 and ICAM4. Functional analysis of MSC proliferation, migration and adhesion to extracellular matrix components revealed that IL-1β did not affect proliferation whereas constitute a throphic factor for MSC and increases adhesion mainly to collagen and laminin. Moreover, IL-1β treatment enhanced the ability of MSC to recruit monocytes and granulocytes cells in vitro. Blockade of NFkβ transcription factor activation with IκB kinase beta (IKKb) siRNA impaired MSC migration, adhesion and leucocyte recruitment, indicating that NF-kB signaling pathway is the main mechanism implicated in these biological processes. These findings are relevant for designing protocols that implicate MSC delivery into inflammatory environments. Overall design: Comparison between two experimen...
  18. A Molecular Signature Associated with Human Melanoma Brain Metastasis and Spontaneous Micrometastasis BioProject

    ID: PRJNA137829

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: , COX-2, MMP1, MMP2 and PRAME and lower levels of CLDN1, CYR61 and IL-6R than the cutaneous variants. These gene products may be involved in melanoma brain metastasis and may serve as novel brain metastasis biomarkers and targets for therapy. Overall design: 8 Samples (arrays) were analyzed. We generated pairwise comparisons between cutaneous and brain metastatic variants of the same genetic background, using Partek Genomics Suite, in the three melanoma models. Genes with p≤5% and a fold-change difference of ≥1.25 or <-1.25 were selected....
  19. Effect of IL-1β on mesenchymal stem cells OmicsDI

    ID: E-GEOD-33755

    Date Released: 07-17-2012

    Description: L-8, IL23A, IL32, Toll-like receptors TLR2, TLR4, CLDN1, metalloproteins MMP1 and MMP3, growth factors CSF2 and TNF, together with adhesion molecules ICAM1 and ICAM4. Functional analysis of MSC proliferation, migration and adhesion to extracellular matrix components revealed that IL-1β did not affect proliferation whereas constitute a throphic factor for MSC and increases adhesion mainly to collagen and laminin. Moreover, IL-1β treatment enhanced the ability of MSC to recruit monocytes and granulocytes cells in vitro. Blockade of NFkβ transcription factor activation with IκB kinase beta (IKKb) siRNA impaired MSC migration, adhesion and leucocyte recruitment, indicating that NF-kB signaling pathway is the main mechanism implicated in these biological processes. These findings are relevant for designing protocols that implicate MSC delivery into inflammatory environments. Comparison between two experimental conditions, ...

  20. Downregulation of CK2-Beta is sufficient to drive epithelial-to-mesenchymal transition through Snail 1 induction OmicsDI

    ID: E-GEOD-28569

    Date Released: 05-03-2014

    Description: ted, whereas epithelial genes such as CDH1, CDH3, CLDN1, CLDN7, OCLN, KRT5, KRT6B, COL2A1 and MUC1, were inversely turned down. The expression of the EMT-inducing transcription factors, Snail and Zeb, was also significantly up-regulated in accordance with the repression of their known target genes. While showing similar phenotypes, the genetic program of TGFβ1-treated or CK2β-depleted MCF10A cells also exhibited distinct features. These results highlight that in addition to TGFβ1-regulated genes, CK2β-depletion may affect alternative pathways. The microarray analysis was performed with RNAs isolated from parental MCF10A cells and the following MCF10A-derived cells: ΔCK2β- and TGFβ1-treated cells (2ng/ml for 72h) and each from two independent cell cultures (duplicates)....


Displaying 20 of 32 results for "CLDN1"