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Displaying 6 of 6 results for "CARD10"
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  1. Lipopolysaccharide-induced microRNA-146a targets CARD10 and involves in angiogenesis of human umbilical vein endothelial cells BioProject

    ID: PRJNA218514

    Keywords: Transcriptome or Gene expression

    Access Type: download

  2. Lipopolysaccharide-induced microRNA-146a targets CARD10 and involves in angiogenesis of human umbilical vein endothelial cells ArrayExpress

    ID: E-GEOD-50619

    Description: or pre-miR-146a into the HUVEC validated that the CARD10 was the target gene of the miR-146a both in the transcript and protein level. immunoprecipitation of RNA-induced silencing complexes (RISC) with an Ago2-specific monoclonal antibody followed by RNA extraction and subsequent quantification of mRNAs on microarrays was utilized to identify mRNAs that are associated with the RNA-silencing machinery and are therefore targets of cellular miRNAs. In brief, 100 μg of total protein was diluted with 200 μL of PBS buffer (pH 7.4). For each sample, 25 μL of Protein A/G plus agarose (Santa Cruz, Dallas, TX) was washed with PBS and incubated with 2 μg of rabbit anti-Ago2 (Abcam, Cambridge, MA) or rabbit normal IgG (Santa Cruz) antibodies for 2 h at 4°C. The beads containing the immobilized anti-Ago2 antibody were then added to 400 μL of diluted serum and incubated for 4 h at 4°C. The beads were washed 3 times with 1% NT-2 buffer (1% Nonidet P-40, 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 2 mM EDTA) and the mixture was split in half. E...

  3. Lipopolysaccharide-induced microRNA-146a targets CARD10 and involves in angiogenesis of human umbilical vein endothelial cells OmicsDI

    ID: E-GEOD-50619

    Date Released: 06-03-2014

    Description: or pre-miR-146a into the HUVEC validated that the CARD10 was the target gene of the miR-146a both in the transcript and protein level. immunoprecipitation of RNA-induced silencing complexes (RISC) with an Ago2-specific monoclonal antibody followed by RNA extraction and subsequent quantification of mRNAs on microarrays was utilized to identify mRNAs that are associated with the RNA-silencing machinery and are therefore targets of cellular miRNAs. In brief, 100 μg of total protein was diluted with 200 μL of PBS buffer (pH 7.4). For each sample, 25 μL of Protein A/G plus agarose (Santa Cruz, Dallas, TX) was washed with PBS and incubated with 2 μg of rabbit anti-Ago2 (Abcam, Cambridge, MA) or rabbit normal IgG (Santa Cruz) antibodies for 2 h at 4°C. The beads containing the immobilized anti-Ago2 antibody were then added to 400 μL of diluted serum and incubated for 4 h at 4°C. The beads were washed 3 times with 1% NT-2 buffer (1% Nonidet P-40, 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 2 mM EDTA) and the mixture was split in half. E...

  4. Global DNA Methylation Levels in Lung Fibroblasts from Patients with Idiopathic Pulmonary Fibrosis Compared to Nonfibrotic Controls BioProject

    ID: PRJNA242344

    Keywords: Epigenomics

    Access Type: download

    dataset.description: erify DNA methylation changes in 3 genes (CDKN2B, CARD10, and MGMT); these methylation changes corresponded with changes in gene expression at the mRNA and protein level. These changes in DNA methylation were stable throughout multiple cell passages. DNA methylation changes may thus help to explain a proportion of the differences in gene expression previously observed in studies of IPF fibroblasts. Moreover, significant variability in DNA methylation was observed among individual IPF cell lines, suggesting that differences in DNA methylation may contribute to fibroblast heterogeneity among patients with IPF. These results demonstrate that IPF fibroblasts exhibit global differences in DNA methylation that may contribute to the excessive fibroproliferation associated with this disease. Overall design: Bisulfite converted DNA from the 12 samples were hybridized to the Illumina Infinium 27k Human Methylation Beadchip v1.2...
  5. Oncoarray Consortium - Lung Cancer Studies dbGaP

    ID: phs001273.v1.p1

    Description: ollution and passive smoking), diet, personal and family history of cancer, and occupational history from each participant. Peripheral blood samples (or mouthwash samples when they refused to donate blood) were collected from all participants. Coding of histology was based on 2001 WHO/IASLC. Genomic DNA was extracted based on standard protocol. The Canadian screening study. It includes the nested case-control samples from 3 screening programs: IELCAP-Toronto: Ever smokers of more than 10 pack-years age 50 and above were eligible for the I-ELCAP screening program since 2003, and a total of 4782 individuals have been enrolled in the Greater Toronto Area. Participants were administered a LDCT scan along with a standard study questionnaire at baseline. Blood samples were systematically collected at baseline since 2006. Participants who had an abnormality in a CT scan were followed up every 1 to 2 years. The screening program was organized by the Princess Margaret Hospital. PanCan: Ever smokers between the ages of 50-75 with no previous history of invasive cancer are eligible to participate in the study. The study was carried out across Canada in Vancouver, Calgary, Hamilton, Toronto, Ottawa, Quebec, Halifax, and St. John's. A total of 2537 smokers have been screened from 2008 to 2011. All study participants completed a detailed questionnaire, spirometry, collection of blood specimens for biomarker measurement and LDCT at baseline. All participants are followed for a minimum of 3 years. On yearly follow up, an updated shorter questionnaire is administered, blood is collected and CT scans are performed. Blood samples are available from all 2537 individuals. BCCA Screening Program: From 1990 to 2007, 4274 smokers above 40 years old who had smoked 20 pack-years or more were enrolled at BCCA. Upon enrollment, subjects completed a questionnaire for their lifestyle and medical history. Baseline spirometry was conducted using a flow-sensitive spirometer in accordance with the American Thoracic Society recommendations. Since 2000, a LDCT was obtained in 2440 individuals. The participants were followed prospectively to determine whether they developed lung cancer. A total of 9759 individuals participated in the CT screening program in Canada from these 3 programs. The samples included in this project is based on a subset of n...

    Study Types: Case-Control

  6. Data used for the analysis of CoMeta program Dataverse

    Description: ssification of metagenomic shotgun sequences with CARMA3. Nucleic acids research 39. [Str] Stranneheim H, Käller M, Allander T, Andersson B, Arvestad L, Lundeberg J (2010) Classification of DNA sequences using Bloom filters. Bioinformatics 26: 1595-1600. [Liu] Liu B, Gibbons T, Ghodsi M, Pop M (2010) MetaPhyler: Taxonomic profiling for metagenomic sequences. In: Proceedings of the 2010 IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2010. pp. 95-100. [Pat] Patil KR, Haider P, Pope PB, Turnbaugh PJ, Morrison M, Scheffer T, McHardy AC (2011) Taxonomic metagenome sequence assignment with structured output models. Nature Methods 8: 191-192. [BaCu] Bazinet A, Cummings M (2012) A comparative evaluation of sequence classification programs. BMC Bioinformatics 13: 1-13. [WoSa] Wood DE, Salzberg SL: Kraken: ultrafast metagenomic sequence classification using exact alignments. Genome Biology 2014, 15:R46....

    Person: Kawulok, Jolanta Deorowicz, Sebastian

    Release Date: 03-02-2015


Displaying 6 of 6 results for "CARD10"