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Displaying 4 of 4 results for "CARD10"
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  1. Lipopolysaccharide-induced microRNA-146a targets CARD10 and involves in angiogenesis of human umbilical vein endothelial cells BioProject

    ID: PRJNA218514

    Keywords: Transcriptome or Gene expression

    Access Type: download

  2. Lipopolysaccharide-induced microRNA-146a targets CARD10 and involves in angiogenesis of human umbilical vein endothelial cells ArrayExpress

    ID: E-GEOD-50619

    Description: or pre-miR-146a into the HUVEC validated that the CARD10 was the target gene of the miR-146a both in the transcript and protein level. immunoprecipitation of RNA-induced silencing complexes (RISC) with an Ago2-specific monoclonal antibody followed by RNA extraction and subsequent quantification of mRNAs on microarrays was utilized to identify mRNAs that are associated with the RNA-silencing machinery and are therefore targets of cellular miRNAs. In brief, 100 μg of total protein was diluted with 200 μL of PBS buffer (pH 7.4). For each sample, 25 μL of Protein A/G plus agarose (Santa Cruz, Dallas, TX) was washed with PBS and incubated with 2 μg of rabbit anti-Ago2 (Abcam, Cambridge, MA) or rabbit normal IgG (Santa Cruz) antibodies for 2 h at 4°C. The beads containing the immobilized anti-Ago2 antibody were then added to 400 μL of diluted serum and incubated for 4 h at 4°C. The beads were washed 3 times with 1% NT-2 buffer (1% Nonidet P-40, 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 2 mM EDTA) and the mixture was split in half. E...

  3. Lipopolysaccharide-induced microRNA-146a targets CARD10 and involves in angiogenesis of human umbilical vein endothelial cells OmicsDI

    ID: E-GEOD-50619

    Date Released: 06-03-2014

    Description: or pre-miR-146a into the HUVEC validated that the CARD10 was the target gene of the miR-146a both in the transcript and protein level. immunoprecipitation of RNA-induced silencing complexes (RISC) with an Ago2-specific monoclonal antibody followed by RNA extraction and subsequent quantification of mRNAs on microarrays was utilized to identify mRNAs that are associated with the RNA-silencing machinery and are therefore targets of cellular miRNAs. In brief, 100 μg of total protein was diluted with 200 μL of PBS buffer (pH 7.4). For each sample, 25 μL of Protein A/G plus agarose (Santa Cruz, Dallas, TX) was washed with PBS and incubated with 2 μg of rabbit anti-Ago2 (Abcam, Cambridge, MA) or rabbit normal IgG (Santa Cruz) antibodies for 2 h at 4°C. The beads containing the immobilized anti-Ago2 antibody were then added to 400 μL of diluted serum and incubated for 4 h at 4°C. The beads were washed 3 times with 1% NT-2 buffer (1% Nonidet P-40, 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 2 mM EDTA) and the mixture was split in half. E...

  4. Global DNA Methylation Levels in Lung Fibroblasts from Patients with Idiopathic Pulmonary Fibrosis Compared to Nonfibrotic Controls BioProject

    ID: PRJNA242344

    Keywords: Epigenomics

    Access Type: download

    dataset.description: erify DNA methylation changes in 3 genes (CDKN2B, CARD10, and MGMT); these methylation changes corresponded with changes in gene expression at the mRNA and protein level. These changes in DNA methylation were stable throughout multiple cell passages. DNA methylation changes may thus help to explain a proportion of the differences in gene expression previously observed in studies of IPF fibroblasts. Moreover, significant variability in DNA methylation was observed among individual IPF cell lines, suggesting that differences in DNA methylation may contribute to fibroblast heterogeneity among patients with IPF. These results demonstrate that IPF fibroblasts exhibit global differences in DNA methylation that may contribute to the excessive fibroproliferation associated with this disease. Overall design: Bisulfite converted DNA from the 12 samples were hybridized to the Illumina Infinium 27k Human Methylation Beadchip v1.2...

Displaying 4 of 4 results for "CARD10"