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Displaying 20 of 25 results for "ASPN"
  1. Asporin is Expressed at Higher Levels in the More Degenerate Human Intervertebral Disc Tissue

    ID: PRJNA116483

    Keywords: Transcriptome or Gene expression

    Access Type: download

  2. Asporin is Expressed at Higher Levels in the More Degenerate Human Intervertebral Disc Tissue

    ID: E-GEOD-15227

    Date Released: 06-27-2012

    Description: Asporin, also known as periodontal ligament-associated protein 1 (PLAP1), is a member of the family of small leu...

  3. Shared gene expression profiles in developmental heart valve remodeling and osteoblast progenitor cells

    ID: PRJNA107137

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: ulogenesis were members of the small leucine-rich proteoglycan (SLRP) family including Asporin, a known negative regulator of osteoblast differentiation and mineralization. Together, these data support shared gene expression profiles of the remodeling valves and osteoblast bone precursor cells in normal valve development and homeostasis with potential functions in calcific valve disease. Keywords: Embryonic valve development time point Overall design: In the study, we hybridized RNA from E12.5 AV cushion and E17.5 AV valve from wild-type FVB/N mice and in vitro cultured MC3T3 cells to Affymetrix MOE430 2 GeneChip® arrays....
  4. Hs_Phospho_AspN_CID

    ID: PAe005276

    Date Released: 12-31-2015

    Description: Multiple protease based human phosphopeptide, AspN, CID

  5. SHR_AspN_Velos

    ID: PAe004320

    Date Released: 12-31-2013

    Description: SHR_liver, AspN, LTQ Orbi Velos

  6. BN_AspN_Velos

    ID: PAe004324

    Date Released: 12-31-2013

    Description: BN-Lx_liver, AspN, LTQ Orbi Velos

  7. SHR_AspN_TTOF

    ID: PAe004330

    Date Released: 12-31-2013

    Description: SHR_liver, AspN, ABI TripleTOF

  8. Hs_Hela_AspN_CID

    ID: PAe005008

    Date Released: 12-31-2014

    Description: Human hela proteome, AspN CID

  9. Hs_Phospho_AspN_ETD

    ID: PAe005250

    Date Released: 12-31-2015

    Description: Multiple protease based human phosphopeptide, AspN, ETD

  10. BN_AspN_TTOF

    ID: PAe004329

    Date Released: 12-31-2013

    Description: BN-Lx_liver, AspN, ABI TripleTOF

  11. Hs_Hela_AspN_HCD

    ID: PAe005048

    Date Released: 12-31-2014

    Description: Human hela proteome, AspN HCD

  12. Gene Expression Analyses of Subchondral Bone in Early Experimental Osteoarthritis by Microarray

    ID: E-GEOD-30322

    Date Released: 06-02-2014

    Description: Igf1, Tgf β1, Postn, Mmp3, Tnfsf11, Acp5, Bmp5, Aspn and Ihh, were confirmed by real-time PCR, and results indicated that our microarray data could accurately reflect gene expression patterns of early OA. Subsequently, to validate the results of our microarray analysis at protein level, immunohistochemistry staining was introduced to investigate the translational level of genes Mmp3 and Aspn in tissue sections, and results showed that the level of Mmp3 protein expression was totally matched the results of microarray and real-time PCR analysis. Nevertheless, the expression of Aspn protein was not observed differentially expressed at any time point. Ninety 10-week-old male Sprague-Dawley rats, weighing 300-325g, were used in the study. Animals were equally divided into two groups: experimental group (E-Group) and sham-operated group (S-Group). The E-Group rats underwent open surgery, involved in both medial meniscectomy and medial collateral ligament (MCL)...

  13. Gene Expression Profiling of Peri-implant Healing of PLGA-Li+ Implants Suggests an Activated Wnt Signaling Pathway in vivo

    ID: PRJNA236109

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: SL1, and the extracellular protein-encoding gene, ASPN, were significantly upregulated by Li+ compared with Ctrl. The presence of β-catenin, FOSL1 and ASPN positive cells was confirmed around implants of both groups. Interestingly, a significantly reduced bone area was observed over time around both implant groups. The presence of periostin and calcitonin receptor-positive cells was observed at both time points. This study is to the best of the authors’ knowledge the first report evaluating the effect of a local release of Li+ from PLGA at the fracture site. The present study shows that during the current time frame and with the present dose of Li+ in PLGA implants, Li+ is not an enhancer of early bone growth, although it affects the Wnt signaling pathway. Overall design: PLGA implants with +/- incorporated Li were inserted in rat tibia for 7 or 28 days, peri-implant bone was harvested and RNA extracted using Qiazol lysis reagent and TissueLyser followed by Qiagen's Rneasy Micro Kit. The microarray experiment was conducted at SCIBLU Genomics (www.lu.se/sciblu) according to Affymetrix guidelines (n=6)....
  14. Gene Expression Analyses of Subchondral Bone in Early Experimental Osteoarthritis by Microarray

    ID: PRJNA143609

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: Igf1, Tgf β1, Postn, Mmp3, Tnfsf11, Acp5, Bmp5, Aspn and Ihh, were confirmed by real-time PCR, and results indicated that our microarray data could accurately reflect gene expression patterns of early OA. Subsequently, to validate the results of our microarray analysis at protein level, immunohistochemistry staining was introduced to investigate the translational level of genes Mmp3 and Aspn in tissue sections, and results showed that the level of Mmp3 protein expression was totally matched the results of microarray and real-time PCR analysis. Nevertheless, the expression of Aspn protein was not observed differentially expressed at any time point. Overall design: Ninety 10-week-old male Sprague-Dawley rats, weighing 300-325g, were used in the study. Animals were equally divided into two groups: experimental group (E-Group) and sham-operated group (S-Group). The E-Group rats underwent open surgery, involved in both medial meniscectomy and medial collatera...
  15. Transcription profiling of mouse E12.5 AV cushion and E17.5 AV valve from wild-type FVB/N mice and in vitro cultured MC3T3 cells

    ID: E-GEOD-11040

    Date Released: 03-27-2012

    Description: ulogenesis were members of the small leucine-rich proteoglycan (SLRP) family including Asporin, a known negative regulator of osteoblast differentiation and mineralization. Together, these data support shared gene expression profiles of the remodeling valves and osteoblast bone precursor cells in normal valve development and homeostasis with potential functions in calcific valve disease. Experiment Overall Design: In the study, we hybridized RNA from E12.5 AV cushion and E17.5 AV valve from wild-type FVB/N mice and in vitro cultured MC3T3 cells to Affymetrix MOE430 2 GeneChip® arrays....

  16. Gene expression changes in NFAT5/TonEBP under isotonic and hypertonic conditions

    ID: PRJNA135623

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: e experiments showed that some of these genes – asporin, insulin-like growth factor-binding protein 5 and 7, and an extracellular lysophosphlipase D – plus Hsp70, a known TonEBP target gene, contributed to the adaptation to hypertonicity without promoting organic osmolyte accumulation. We conclude that TonEBP stimulates multiple cellular pathways for adaptation to hypertonic stress in addition to organic osmolyte accumulation. Overall design: Quadruplicate samples were collected for each condition and then pooled into a single sample for hybridization to microarrays....
  17. Gene Expression Profiling of Peri-implant Healing of PLGA-Li+ Implants Suggests an Activated Wnt Signaling Pathway in vivo.

    ID: E-GEOD-54294

    Date Released: 07-26-2014

    Description: SL1, and the extracellular protein-encoding gene, ASPN, were significantly upregulated by Li+ compared with Ctrl. The presence of β-catenin, FOSL1 and ASPN positive cells was confirmed around implants of both groups. Interestingly, a significantly reduced bone area was observed over time around both implant groups. The presence of periostin and calcitonin receptor-positive cells was observed at both time points. This study is to the best of the authors’ knowledge the first report evaluating the effect of a local release of Li+ from PLGA at the fracture site. The present study shows that during the current time frame and with the present dose of Li+ in PLGA implants, Li+ is not an enhancer of early bone growth, although it affects the Wnt signaling pathway. PLGA implants with +/- incorporated Li were inserted in rat tibia for 7 or 28 days, peri-implant bone was harvested and RNA extracted using Qiazol lysis reagent and TissueLyser followed by Qiagen's Rneasy Micro Kit. The microarray experiment was conducted at SCIBLU Genomics (www.lu.se/sciblu) according to Affymetrix guidelines (n=6)....

  18. Proteogenomic analysis of Helicobacter pylori strain 26695

    ID: PXD000054

    Date Released: 08-01-2013

    Description: SEC fractionation of LMW proteins and subsequent AspN digestion, replicate 1 A2: SEC fractionation of LMW proteins and subsequent AspN digestion, replicate 2 L1: SEC fractionation of LMW proteins and subsequent LysC digestion, replicate 1 L2: SEC fractionation of LMW proteins and subsequent LysC digestion, replicate 2 In our proteogenomics approach, we could identify four previously missing protein annotations and were able to correct sequences of six protein coding regions. Furthermore we identified signal peptidase cleavage sites for 72 different proteins. MGFs were generated by Maxquant 1.1 [1] using recalibration of peptide parent masses. For PRIDE (http://www.e...

  19. Combining solid phase extraction with ultra-short gradients in LC-MS/MS substantially improves protein identification per unit of time

    ID: PXD001111

    Date Released: 12-12-2014

    Description: mics experiment, involving 36 SCX fractions of an AspN digested cell lysate, lead to the detection of over 3600 protein groups in only 3h cumulative gradient time when using a QExactive mass analyser. Application to typical various proteomics experiments such as samples from pull-downs allowed the detection of hundreds of proteins whereby a given protein’s interactome could be determined in less than 1h of accumulated gradient time. This new LC system diminishes proteome analysis time considerably, whole providing sufficient proteomic detail, and may thus also aid in bringing proteomics into the clinic where fast turn-over times are essential....

  20. miRNA expression profiling in neural differentiated mouse embryonic stem cells (mESCs) under exposure to sodium valproate and sodium arsenite

    ID: PRJNA217306

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: cific genes (Actc1, calponin, myosin light chain, asporin, decorin) and repression of genes involved in neurogenesis (Otx1 and 2, Zic3, 4, 5)) as well as morphologically by immunocytochemistry. The observed results were VPA specific and most probably due to inhibition of histone deacetylase (HDAC) activity of VPA for two reasons: (i) we did not observe any induction of muscle specific miRNAs in neural differentiating ES cells exposed to the unrelated developmental neurotoxicant sodium arsenite; (ii) expression of muscle specific mir-206 and muscle enriched mir-10a was similarly increased in cells exposed to a structurally different HDAC inhibitor, trichostatin A (TSA). Furthermore, using our in vitro cell system we could confirm an aberrant expression of known VPA target genes and genes involved in neural tube closure. We conclude that miRNA expression profiling is a suitable molecular endpoint for developmental neurotoxicity. Observed lineage shift into myogenesis, where miRNAs play a significant role, could be a major developmental neurotoxical mechanism of VPA. We used microarray approach to identify altered miRNA expression in neural differentiated mES cells exposed to two known developmental neurotxicants and epigenetic active substances, sodium valproate (VPA) and sodium arsenite (As) Overall design: mES cells line W4 were induced to differnetiate to neurons under exposure to VPA and As for 16 days. RNA for microarrays was collected on day 16 of differentiation from three biological replicates of solvent controls (...

Displaying 20 of 25 results for "ASPN"