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Displaying 20 of 21 results for "ALAS1"
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  1. Figure4.ALAS Dryad

    DateIssued: 04-07-2016

  2. SOLUTION STRUCTURE OF THE 49 AA PRESEQUENCE OF 5-ALAS PDB

    ID: PDB:1H7D

    Description: AMINOLEVULINIC ACID SYNTHASE 2, ERYTHROID (E.C.2.3.1.37)

  3. SOLUTION STRUCTURE OF THE 26 AA PRESEQUENCE OF 5-ALAS PDB

    ID: PDB:1H7J

    Description: AMINOLEVULINIC ACID SYNTHASE 2, ERYTHROID (E.C.2.3.1.37)

  4. HEM1_OPSTA UniProt:Swiss-Prot

    ID: P43091

    Description: Mitochondrion 5-aminolevulinate synthase, nonspecific, mitochondrial Substrate Substrate Substrate Pyridoxal phosphate Pyridoxal phosphate Pyridoxal p...

    gene.name: alas1
  5. HEM1_PONAB UniProt:Swiss-Prot

    ID: Q5R9R9

    Description: Mitochondrion 5-aminolevulinate synthase, nonspecific, mitochondrial Substrate Substrate Substrate Pyridoxal phosphate Pyridoxal phosphate Pyridoxal p...

    gene.name: ALAS1
  6. HEM1_BOVIN UniProt:Swiss-Prot

    ID: A6QLI6

    Description: Mitochondrion 5-aminolevulinate synthase, nonspecific, mitochondrial Substrate Substrate Substrate Pyridoxal phosphate Pyridoxal phosphate Pyridoxal p...

    gene.name: ALAS1
  7. Project ALAS: Arthropods of La Selva Dryad

    DateIssued: 01-09-2014

    Description: Project ALAS is a large-scale inventory of arthropod diversity along an elevational gradient in Costa Rica, from lowland tropical rainforest through m...

  8. Transcription profiling of human and moise primary hepatocytes from 12 donors were treated with PPARI? agonist Wy14643 ArrayExpress

    ID: E-GEOD-17254

    Description: re specifically induced by PPARα in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARα targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARα activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARα as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARα regulates a mostly divergent set of genes in mouse and human hepatocytes. GSE17250: Comparative analysis of gene regulation by the transcription factor PPARα_mouse; GSE17251: Comparative analysis of gene regulation by the transcription factor PPARα_human Experiment Overall Design: Refer to individual Series...

  9. Transcription profiling of human and mouse hepatocytes to perform a comparative analysis of gene regulation by the transcription factor PPARI?_mouse ArrayExpress

    ID: E-GEOD-17250

    Description: re specifically induced by PPARα in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARα targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARα activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARα as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARα regulates a mostly divergent set of genes in mouse and human hepatocytes. Experiment Overall Design: Primary hepatocytes from 6 mice from 6 different strains were treated with the PPARα agonist Wy14643 for 6 and 24 hours, and gene expression profiling was performed using Affymetrix GeneChips. Mouse primary hepatocytes were isolated by two-step collagenase perfusion from 6 different strains of mouse; NMRI, SV129, FVB, DBA, BALB/C and C57BL/6J. Cells were plated on collagen-coated six-well plates. Viability was determined by Trypan Blue exclusion, and was at least 75%. Hepatocytes were suspended in William's E medium (Lonza Bioscience, Verviers, Belgium) supplemented with 10% (v/v) fetal calf serum, 20 m-units/mL insulin, 10 nM dexamethasone, 100 U/mL penicillin, 100 μg/mL of streptomycin, 0.25 μg/mL fungizone and 50 μg/mL gentamycin. After four hours the medium was discarded and replaced with fresh medium. The nex...

  10. Transcription profiling by array of human primary hepatocytes after treatment with pirinixic acid ArrayExpress

    ID: E-GEOD-17251

    Description: specifically induced by PPARalpha in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARalpha targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARalpha activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARalpha as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARalpha regulates a mostly divergent set of genes in mouse and human hepatocytes. Experiment Overall Design: Primary hepatocytes from 6 human subjects were treated with the PPARalpha agonist Wy14643 for 6 and 24 hours, and gene expression profiling was performed using Affymetrix GeneChips. Human hepatocytes and Hepatocyte Culture Medium Bulletkit were purchased from Lonza Bioscience (Verviers, Belgium). Primary hepatocytes were isolated from surgical liver biopsies obtained from six individual donors who underwent surgery after informed consent was obtained for surgery with subsequent use of samples in experiments. Hepatocytes were isolated with two-step collagenase perfusion method and the viability of the cells was over 80%. Cells were plated on collagen-coated six-well plates and filled with maintenance medium. Upon arrival of the cells, the medium was discarded and was replaced by Hepatocyte Culture Medium (HCM) with additives. The a...

  11. Comparative analysis of gene regulation by the transcription factor PPARα between mouse and human BioProject

    ID: PRJNA118935

    Access Type: download

    dataset.description: re specifically induced by PPARα in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARα targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARα activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARα as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARα regulates a mostly divergent set of genes in mouse and human hepatocytes. This SuperSeries is composed of the SubSeries listed below. Overall design: Refer to individual Series...
  12. Transcription profiling of human and moise primary hepatocytes from 12 donors were treated with PPARI? agonist Wy14643 OmicsDI

    ID: E-GEOD-17254

    Date Released: 03-27-2012

    Description: re specifically induced by PPARα in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARα targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARα activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARα as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARα regulates a mostly divergent set of genes in mouse and human hepatocytes. GSE17250: Comparative analysis of gene regulation by the transcription factor PPARα_mouse; GSE17251: Comparative analysis of gene regulation by the transcription factor PPARα_human Experiment Overall Design: Refer to individual Series...

  13. Comparative analysis of gene regulation by the transcription factor PPARα_human BioProject

    ID: PRJNA123587

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: re specifically induced by PPARα in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARα targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARα activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARα as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARα regulates a mostly divergent set of genes in mouse and human hepatocytes. Keywords: Analysis of target gene regulation by using microarrays Overall design: Primary hepatocytes from 6 human subjects were treated with the PPARα agonist Wy14643 for 6 and 24 hours, and gene expression profiling was performed using Affymetrix GeneChips. Human hepatocytes and Hepatocyte Culture Medium Bulletkit were purchased from Lonza Bioscience (Verviers, Belgium). Primary hepatocytes were isolated from surgical liver biopsies obtained from six individual donors who underwent surgery after informed consent was obtained for surgery with subsequent use of samples in experiments. Hepatocytes were isolated with two-step collagenase perfusion method and the viability of the cells was over 80%. Cells were plated on collagen-coated six-well plates and filled with maintenance medium. Upon arrival of the cells, the medium was discarded and was r...
  14. Comparative analysis of gene regulation by the transcription factor PPAR? between mouse and human - Homo sapiens GEMMA

    ID: 1517

    Keywords: functional genomics

    Description: specifically induced by PPARalpha in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARalpha targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARalpha activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARalpha as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARalpha regulates a mostly divergent set of genes in mouse and human hepatocytes. GSE17254.2: Comparative analysis of gene regulation by the transcription factor PPARalpha_mouse GSE17254.1: Comparative analysis of gene regulation by the transcription factor PPARalpha_human Last Updated (by provider): Jan 15 2010 Contributers: Maryam Rakhshandehroo Michael Müller Sander Kersten Guido Hooiveld...

  15. Comparative analysis of gene regulation by the transcription factor PPARalpha between mouse and human - Mus musculus GEMMA

    ID: 1516

    Keywords: functional genomics

    Description: specifically induced by PPARalpha in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARalpha targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARalpha activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARalpha as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARalpha regulates a mostly divergent set of genes in mouse and human hepatocytes. GSE17254.2: Comparative analysis of gene regulation by the transcription factor PPARalpha_mouse GSE17254.1: Comparative analysis of gene regulation by the transcription factor PPARalpha_human Last Updated (by provider): Jan 15 2010 Contributers: Maryam Rakhshandehroo Michael Müller Sander Kersten Guido Hooiveld...

  16. Comparative analysis of gene regulation by the transcription factor PPARα_mouse BioProject

    ID: PRJNA123585

    Keywords: Transcriptome or Gene expression

    Access Type: download

    dataset.description: re specifically induced by PPARα in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARα targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARα activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARα as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARα regulates a mostly divergent set of genes in mouse and human hepatocytes. Keywords: Analysis of target gene regulation by using microarrays Overall design: Primary hepatocytes from 6 mice from 6 different strains were treated with the PPARα agonist Wy14643 for 6 and 24 hours, and gene expression profiling was performed using Affymetrix GeneChips. Mouse primary hepatocytes were isolated by two-step collagenase perfusion from 6 different strains of mouse; NMRI, SV129, FVB, DBA, BALB/C and C57BL/6J. Cells were plated on collagen-coated six-well plates. Viability was determined by Trypan Blue exclusion, and was at least 75%. Hepatocytes were suspended in William's E medium (Lonza Bioscience, Verviers, Belgium) supplemented with 10% (v/v) fetal calf serum, 20 m-units/mL insulin, 10 nM dexamethasone, 100 U/mL penicillin, 100 μg/mL of streptomycin, 0.25 μg/mL fungizone and 50 μg/mL gentamycin. After four hours the mediu...
  17. Transcription profiling by array of human primary hepatocytes after treatment with pirinixic acid OmicsDI

    ID: E-GEOD-17251

    Date Released: 07-28-2015

    Description: specifically induced by PPARalpha in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARalpha targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARalpha activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARalpha as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARalpha regulates a mostly divergent set of genes in mouse and human hepatocytes. Experiment Overall Design: Primary hepatocytes from 6 human subjects were treated with the PPARalpha agonist Wy14643 for 6 and 24 hours, and gene expression profiling was performed using Affymetrix GeneChips. Human hepatocytes and Hepatocyte Culture Medium Bulletkit were purchased from Lonza Bioscience (Verviers, Belgium). Primary hepatocytes were isolated from surgical liver biopsies obtained from six individual donors who underwent surgery after informed consent was obtained for surgery with subsequent use of samples in experiments. Hepatocytes were isolated with two-step collagenase perfusion method and the viability of the cells was over 80%. Cells were plated on collagen-coated six-well plates and filled with maintenance medium. Upon arrival of the cells, the medium was discarded and was replaced by Hepatocyte Culture Medium (HCM) with additives. The a...

  18. Transcription profiling of human and mouse hepatocytes to perform a comparative analysis of gene regulation by the transcription factor PPARI?_mouse OmicsDI

    ID: E-GEOD-17250

    Date Released: 06-10-2011

    Description: re specifically induced by PPARα in human (MBL2, ALAS1, CYP1A1, TSKU) or mouse (Fbp2, lgals4, Cd36, Ucp2, Pxmp4). Furthermore, several putative novel PPARα targets were identified that were commonly regulated in both species, including CREB3L3, KLF10, KLF11 and MAP3K8. Our results suggest that PPARα activation has a major impact on gene regulation in human hepatocytes. Importantly, the role of PPARα as master regulator of hepatic lipid metabolism is generally well-conserved between mouse and human. Overall, however, PPARα regulates a mostly divergent set of genes in mouse and human hepatocytes. Experiment Overall Design: Primary hepatocytes from 6 mice from 6 different strains were treated with the PPARα agonist Wy14643 for 6 and 24 hours, and gene expression profiling was performed using Affymetrix GeneChips. Mouse primary hepatocytes were isolated by two-step collagenase perfusion from 6 different strains of mouse; NMRI, SV129, FVB, DBA, BALB/C and C57BL/6J. Cells were plated on collagen-coated six-well plates. Viability was determined by Trypan Blue exclusion, and was at least 75%. Hepatocytes were suspended in William's E medium (Lonza Bioscience, Verviers, Belgium) supplemented with 10% (v/v) fetal calf serum, 20 m-units/mL insulin, 10 nM dexamethasone, 100 U/mL penicillin, 100 μg/mL of streptomycin, 0.25 μg/mL fungizone and 50 μg/mL gentamycin. After four hours the medium was discarded and replaced with fresh medium. The nex...

  19. Early life stress perturbs the maturation of microglial in the developing hippocampus [P28] BioProject

    ID: PRJNA320322

    Keywords: Other

    Access Type: download

    dataset.description: positive controls and 5 housekeeping genes (e.g. Alas1, Gapdh, Oaz1, Polr1b, Sdha, Tbp) that were not affected by either BDS or age and showed a coefficient of variation of less than 20%. Genes were considered “present” if at least 40% of the samples showed hybridization signal that was 2 s.d. above the mean value for the negative control probes...
  20. Early life stress perturbs the maturation of microglia in the developing hippocampus [P14] BioProject

    ID: PRJNA320323

    Keywords: Other

    Access Type: download

    dataset.description: positive controls and 5 housekeeping genes (e.g. Alas1, Gapdh, Oaz1, Polr1b, Sdha, Tbp) that were not affected by either BDS or age and showed a coefficient of variation of less than 20%. Genes were considered “present” if at least 40% of the samples showed hybridization signal that was 2 s.d. above the mean value for the negative control probes...

Displaying 20 of 21 results for "ALAS1"