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Title: Chicken plasma proteome LPS      
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available
aggregation:
instance of dataset
privacy:
not applicable
refinement:
curated
dateReleased:
01-01-1000
ID:
PXD003335
description:
Lipopolysaccharides (LPS) are cell wall components of gram-negative bacteria and endotoxins that cause inflammation and sickness in vertebrate animals. Our objective to identify the plasma proteomic changes in an avian model of inflammation. Chickens were treated with either saline or LPS and the blood was collected 24 hours after injection. The pooled plasma samples were depleted of high abundant proteins, analyzed by Matrix assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography–tandem mass spectrometry (LC-MS/MS). MALDI-TOF analyses showed an increase in fibrinogen beta derived peptide and decrease in apolipoprotein AII- isoform X1 derived peptide in LPS samples. Label free quantitation of LC-MS/MS spectra revealed an increase in alpha-1 acid glycoprotein, chemokine-CCLI10, and cathelicidin-2 and decrease in interferon stimulated gene-12-2 in the LPS group. These differentially expressed proteins are associated with inflammation, cytokines, immunomodulation, and defense which are useful as candidate biomarkers of infection and inflammation.
keywords:
acute phase proteins
innate immunity
biomarkers
endotoxin
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HTML
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Pride
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accessType:
landing page
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none
authentication:
none
primary:
true
accessURL: http://www.ebi.ac.uk/pride/archive/projects/PXD003335
format:
JSON
storedIn:
OmicsDI
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accessType:
download
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none
primary:
false
accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/PXD003335.json
format:
XML
storedIn:
OmicsDI
qualifier:
not compressed
accessType:
download
authorization:
none
authentication:
none
primary:
false
accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/PXD003335.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/