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Title: Myosin VIIA 138-1 immunoaffinity purification      
availability:
available
aggregation:
instance of dataset
privacy:
not applicable
refinement:
curated
dateReleased:
01-01-1000
ID:
PXD004221
description:
Through its activity in stereocilia, MYO7A (myosin VIIA) is essential for hair cell function in the inner ear. Utilizing multiple stages of immunoaffinity enrichment, we have developed a strategy that allows us to partially purify stereocilia membranes from thousands of chick inner ears and isolate low-abundance MYO7A protein complexes from those membranes. By analysis of MYO7A and co-purifying molecules with shotgun and targeted mass spectrometry, we demonstrated that MYO7A forms a complex with PDZD7, a paralog of USH1C and DFNB31, which has been shown to localize to the ankle-link region of stereocilia.
keywords:
auditory
PDZ
myosin
Hair cell
hair bundle
stereocilia
vestibular
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none
authentication:
none
primary:
true
accessURL: http://www.ebi.ac.uk/pride/archive/projects/PXD004221
format:
JSON
storedIn:
OmicsDI
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not compressed
accessType:
download
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primary:
false
accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/PXD004221.json
format:
XML
storedIn:
OmicsDI
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not compressed
accessType:
download
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none
authentication:
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primary:
false
accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/PXD004221.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/