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Title: Arabidopsis endomembrane enrichments: Affinity enrichment of YFP-GOT1      
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available
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refinement:
curated
dateReleased:
04-22-2015
ID:
PXD001877
description:
Our aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations of endosomal proteomes associated with endomembrane markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, and RABG3f as markers for combinations of the Golgi, trans-Golgi Network (TGN), Early Endosomes (EE), secretory vesicles, Late Endosomes (LE), Multivesicular Bodies (MVB) compartments and tonoplast. As comparisons we used Golgi transport 1 (GOT1), which localises to the Golgi, Clathrin Light Chain 2 (CLC2) labelling Clathrin-coated vesicles and pits and the Vesicle Associated Membrane Protein 711 (VAMP711) present at the tonoplast. We developed an easy-to-use method by refining published protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker proteins in Arabidopsis thaliana seedlings.
keywords:
clathin
Arabidopsis
small GTPases
affnity purification
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accessURL: http://www.ebi.ac.uk/pride/archive/projects/PXD001877
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accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/PXD001877.json
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accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/PXD001877.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/