Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Transcription profiling of grapevine cell culture treated with methyly jasmonate, salicylic acids and ethanol controls      
availability:
available
aggregation:
instance of dataset
privacy:
not applicable
refinement:
curated
dateReleased:
12-07-2015
ID:
E-MEXP-1524
description:
Calli generated from grape berries were used to establish a cell suspension in Gamborgs B5 liquid medium with minimal organics supplemented with 30 gL-1 sucrose, 0.25 gL-1 casein hydrolysate, 0.93 ?M kinetin and 0.54 ?M naphthaleneacetic acid (NAA) and incubated in darkness at 26°C.
The cells were cultured in 250 mL flasks (50 mL of cell suspension in each) incubated in darkness on an orbital shaker at 100 rpm. The cell suspensions were sub-cultured every 2 weeks using an initial packed cell volume (PCV) of 15%. For the evaluation of the effect of elicitor addition on the changes in gene expression, 100 mL flasks containing 20 mL of the above cell suspension were used. Each treatment was conducted in triplicate.
In a set of preliminary experiments studying the effect of elicitors on the volatiles produced by the cell suspensions, the compounds were added during the exponential growth phase when the PCV was approximately 50%. After 72h incubation in darkness after elicitor addiction, cells were harvested. The elicitors screened for their ability to induce volatile compound production were: 500 µM methyl jasmonate (MeJA); 500 uM MeJA + 500 µM salicylic acid (SA); 500 µM jasmonic acid (JA). Controls were conducted by adding the same volume of the solvent used to dissolve each elicitor to the cultures. For the jasmonate and SA experiments the solvent was ethanol.
keywords:
transcription profiling by array
format:
HTML
storedIn:
Array Express
qualifier:
not compressed
accessType:
landing page
authorization:
none
authentication:
none
primary:
true
accessURL: https://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1524
format:
JSON
storedIn:
OmicsDI
qualifier:
not compressed
accessType:
download
authorization:
none
authentication:
none
primary:
false
accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/E-MEXP-1524.json
format:
XML
storedIn:
OmicsDI
qualifier:
not compressed
accessType:
download
authorization:
none
authentication:
none
primary:
false
accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/E-MEXP-1524.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/