Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Transcription profiling by array of Saccharomyces cerevisiae in response to ethanol      
availability:
available
aggregation:
instance of dataset
privacy:
not applicable
refinement:
curated
dateReleased:
12-11-2015
ID:
E-MTAB-3874
description:
The genome-wide transcriptional responses of the wild type laboratory strain, and two deletion strains (pmt7Δ/pmt7Δ and yhl042wΔ/yhl042wΔ) were comparatively investigated in the absence and in the presence of ethanol for two hours. Strains were cultivated in flasks at 30°C and 180 rpm in an orbital shaker till the mid-exponential phase of growth (OD600 of 0.85-0.95). The exponential cultures were then divided into two flasks and cells in one flask were grown without ethanol, whereas cells in the latter flask were treated with ethanol to have 8% (v/v) final ethanol concentration. In order to analyze the transcriptional response of each strain to ethanol, samples were collected 2 h after ethanol treatment from both treated and untreated cultures, immediately frozen in liquid nitrogen and stored at -80C until RNA isolation. All experiments were carried out in triplicate.
keywords:
transcription profiling by array
format:
HTML
storedIn:
Array Express
qualifier:
not compressed
accessType:
landing page
authorization:
none
authentication:
none
primary:
true
accessURL: https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3874
format:
JSON
storedIn:
OmicsDI
qualifier:
not compressed
accessType:
download
authorization:
none
authentication:
none
primary:
false
accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/E-MTAB-3874.json
format:
XML
storedIn:
OmicsDI
qualifier:
not compressed
accessType:
download
authorization:
none
authentication:
none
primary:
false
accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/E-MTAB-3874.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/