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Title: Cell Image Library Dataset CIL:9971      
dateReleased:
11-30-2010
description:
Normal Rat Kidney (NRK) cells grown in culture expressing Galactosyl Transferase-YFP (GalT-YFP) and p58-CFP. This file is the CFP time series demonstrating the organization of the early secretory pathway (Golgi and intermediate compartment). Images were collected on a Zeiss Spinning Disk confocal as a single z slice over time with CFP imaged using 445ht and YFP imaged using 515ht and a 63X oil objective. Images were collected every 2 minutes. Exposure time: 515 was 50ms; 445 was 200ms: for both channels, the gain was 3, the camera intensification was 200, the laser neutral density was 10.
privacy:
not applicable
aggregation:
instance of dataset
ID:
9971
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
CFP; ECFP
name:
live-cell imaging, living tissue
name:
permanent cell line cell
name:
constitutive secretory pathway
name:
Kidney
name:
NRK
name:
endoplasmic reticulum-Golgi intermediate compartment
name:
ER-Golgi intermediate compartment
name:
Golgi
name:
Golgi apparatus
name:
spinning disk confocal microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/9971.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/9971.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/9971.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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