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Title: Cell Image Library Dataset CIL:837      
dateReleased:
08-05-2010
description:
Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Left image of a stereo pair.
privacy:
not applicable
aggregation:
instance of dataset
ID:
837
refinement:
curated
availability:
available
types:
image
dimensions:
electron density, elevation
keywords:
shadowing and plating
name:
freeze_fracture/freeze_etch, glutaraldehyde fixed tissue
name:
skeletal muscle cell
name:
skeletal muscle contraction
name:
cytoskeleton
name:
detection of electrons, illumination by electrons, transmission electron microscopy (TEM), wide-field detection, widefield illumination
accessURL: www.cellimagelibrary.org/images/download_jpeg/837.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/837.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/837.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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