Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Cell Image Library Dataset CIL:35899      
dateReleased:
08-02-2011
description:
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Lte1. LTE1 is essential for termination of M phase at low temperatures, along with TEM1, and CDC15, all part of the mitotic exit network which is an elaborate signaling system comprising at least eight essential genes: CDC5, CDC14, CDC15, DBF2, DBF20, LTE1, MOB1, and TEM1. LTE1 was originally discovered as a locus essential for growth at 8 C, and was named for this trait as Low Temperature Essential 1. It was later inferred from sequence similarities that LTE1 must code for a guanine nucleotide exchange factor. Genetic evidence suggests that Lte1p activates the GTPase Tem1p which activates the protein kinase Cdc15p. Cdc15p then relieves the inhibition of the protein phosphatase Cdc14p by Net1p, thereby allowing exit from mitosis. In a normal cell cycle, Bub2p bound to Tem1p restrains mitotic exit until the daughter-bound centrosome (with which both Bub2p and Tem1p preferentially associate) comes into contact with Lte1p in the bud, effectively coupling mitotic exit with nuclear segregation. Some evidence suggests that the spatial separation of Tem1p and Lte1p is a key mechanism for controlling the timing of mitotic exit. These phase, gfp images are part of an image group that ranges from CIL:35880-35899. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
privacy:
not applicable
aggregation:
instance of dataset
ID:
35899
refinement:
curated
availability:
available
types:
image
dimensions:
elastic scattering of photons, fluorescence emission
keywords:
EGFP; GFP; visualization of contiguous regions
name:
live-cell imaging, living tissue
name:
mitotic cell cycle spindle orientation checkpoint
name:
nonselective vesicle transport
name:
regulation of exit from mitosis
name:
SOC
name:
vesicle-mediated transport
name:
cellular bud
name:
LTE1 protein
name:
fluorescence microscopy, phase contrast microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/35899.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_cc_by
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/35899.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_cc_by
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/35899.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_cc_by
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.