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Title: Cell Image Library Dataset CIL:35689      
dateReleased:
08-02-2011
description:
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged MAD2. MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, mad2 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad2p forms a tight complex with another spindle checkpoint protein, Mad1p, throughout the cell cycle. Mad2p also forms a complex with Cdc20p, which activates the anaphase promoting complex, and Mad3p; the presence of Mad1p is required for the complex to form. Bub1p, Bub3p, Mad1p, Mad2p, Mad3p, and the protein kinase Mps1p act in a branch of the spindle checkpoint pathway that may prevent premature chromosome disjunction. A second branch involves Bub2p and Bfa1p, and may prevent cytokinesis prior to chromosome segregation. Homologs of Mad2p act in the spindle checkpoint in Xenopus (Xmad2) and human (MAD2L1 and MAD2L2. MAD2 was originally identified as the open reading frame YJL031C. However, it is now known that MAD2 corresponds to the adjacent open reading frame YJL030W, while YJL031C encodes the geranylgeranyltransferase subunit Bet4p. These phase, gfp images are part of an image group that ranges from CIL:35680-35699. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
privacy:
not applicable
aggregation:
instance of dataset
ID:
35689
refinement:
curated
availability:
available
types:
image
dimensions:
elastic scattering of photons, fluorescence emission
keywords:
EGFP; GFP; visualization of contiguous regions
name:
live-cell imaging, living tissue
name:
chromosome decondensation
name:
Mad2-dependent checkpoint
name:
mitotic cell cycle G2/M transition decatenation checkpoint
name:
mitotic cell cycle spindle assembly checkpoint
name:
topo II checkpoint
name:
MAD2 protein
name:
MCC
name:
mitotic checkpoint complex
name:
NPC
name:
nuclear pore
name:
fluorescence microscopy, phase contrast microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/35689.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_cc_by
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/35689.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_cc_by
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/35689.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_cc_by
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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