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Title: Cell Image Library Dataset CIL:35148      
dateReleased:
07-27-2011
description:
This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo expressing H2B::GFP by DIC and fluorescence microscopy. GFP-tagged histone H2B allows chromosome segregation to be followed. In wild-type, DNA replication generates two sets of sister chromatids that segregate toward opposite spindle poles during mitosis. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The series corresponds to experiments shown in Fig. 3A and Fig S5 and Movie 7 of Mouysset et al. Movie is CIL 25634 and original data is CIL 35148.
privacy:
not applicable
aggregation:
instance of dataset
ID:
35148
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission, optical path length gradient
keywords:
EGFP; GFP; visualization of contiguous regions
name:
live-cell imaging, living tissue
name:
early embryonic cell
name:
cell division
name:
mitosis
name:
nuclear morphology
name:
nucleus organization
name:
unc-119(ed3)
name:
cell nucleus
name:
nucleosome
name:
nucleus
name:
DIC, differential interference contrast microscopy, fluorescence microscopy, time lapse microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/35148.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa, copyright
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/35148.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa, copyright
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/35148.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_nc_sa, copyright
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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