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Title: Cell Image Library Dataset CIL:25635      
dateReleased:
08-30-2011
description:
This movie illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 by RNAi expressing H2B::GFP by DIC and fluorescence microscopy. GFP-tagged histone H2B allows chromosome segregation to be followed. In wild-type, DNA replication generates two sets of sister chromatids that segregate toward opposite spindle poles during mitosis. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. In npl-4(RNAi) embryos, the movie shows chromosome bridges during mitosis as well as a delay in chromatin condensation, especially in the P1 cell. The movie corresponds to experiments shown in Fig. 3A and Fig S5 and Movie 8 of Mouysset et al. Movie is CIL 25635 and original data is CIL35149.
privacy:
not applicable
aggregation:
instance of dataset
ID:
25635
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission, optical path length gradient
keywords:
EGFP; GFP; visualization of contiguous regions
name:
live-cell imaging, living tissue
name:
early embryonic cell
name:
cell division
name:
mitosis
name:
nuclear morphology
name:
nucleus organization
name:
unc-119(ed3)
name:
Cdc48p-Npl4p-Ufd1p AAA ATPase complex
name:
cell nucleus
name:
nucleosome
name:
nucleus
name:
DIC, differential interference contrast microscopy, fluorescence microscopy, time lapse microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/25635.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
copyright
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/25635.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
copyright
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/25635.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
copyright
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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