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Title: Cell Image Library Dataset CIL:13998      
dateReleased:
04-02-2011
description:
Hela cells were treated with control siRNA and synchronized by double thymidine block. Metaphase cells were fixed and stained with CREST sera (red, Alexa Flour 568), CenpI antibody (green, Alexa Fluor 488) and Hoechst 33342 (blue). Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm). Image Reference: PMID 20212317
privacy:
not applicable
aggregation:
instance of dataset
ID:
13998
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
Alexa Fluor 488; Alexa Fluor 568; HO342; Hoechst 33342
name:
formaldehyde fixed tissue
name:
cell cycle checkpoint
name:
HeLa
name:
centromere
name:
chromosome
name:
centromeric region
name:
nuclear chromosome
name:
nuclear interphase chromosome
name:
single-spot confocal microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/13998.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13998.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13998.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_nc_sa
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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