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Title: Cell Image Library Dataset CIL:13685      
dateReleased:
04-30-2011
description:
To study the cellular consequences of misfolding of plasma membrane proteins, a transmembrane model protein that was constitutively targeted to the plasma membrane was developed for use as a reporter molecule. The reporter consisted of a C-terminally truncated CD4, incorporating a flexible cytoplasmic linker (CD4tl), fused to the N-terminal DNA-binding domain of the wild type (wt) bacteriophage lambda repressor (CD4tl-lambda) or L57C mutant repressor (CD4tl-lambdaC). The CD4tl-lambdaC cytosolic domain was largely in native state at 26°C but predominantly nonnative state at 37°C thus allowing the use of thermal shifts to follow the fate of unfolded proteins. In this image, one of six of a group from Figure 1 of Pirjo et al., JCB 2010, the expression of the control reporter, CD4tl-lambda, at 37 degrees is seen with CD4 antibody (green) in a non-permeabilized COS7 cell and the apparent plasma membrane targeting shows the value of this model plasma membrane protein. The ER marker, calreticulin antibody (red), is not apparent here because lack of detergent-permeabilization limited access of the antibody to the ER.
privacy:
not applicable
aggregation:
instance of dataset
ID:
13685
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
Alexa Fluor 488; Alexa Fluor 555
name:
crosslinking-fixative fixed tissue
name:
protein-plasma membrane targeting
name:
protein targeting to plasma membrane
name:
COS7
name:
COS-7
name:
endoplasmic reticulum
name:
ER
name:
integral to plasma membrane
name:
confocal microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/13685.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13685.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13685.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_nc_sa
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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