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Title: Cell Image Library Dataset CIL:13436      
dateReleased:
04-27-2011
description:
FISH detection of ERBB2 (florescein, green) and MYC (rhodamine, red) in normal breast tissue. Nuclei counterstained with DAPI (blue). Tissue was fixed in formaldehyde, embedded in paraffin, and 4-5 micron sections cut. Stained sections were viewed using an Olympus IX70 microscope with a 60x 1.4 NA objective lens, and images acquired with a Photometrics CoolSnap CCD with a 1024X1024 pixel resolution, with a pixel size equivalent to 0.07427 microns in X and Y. Shown is a maximum intensity projection of the stack. The complete stack is included in this group of images Part of this field of view is shown in Fig 1B of Meaburn et al., 2009, J Cell Biol 187:801-812.
privacy:
not applicable
aggregation:
instance of dataset
ID:
13436
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
4',6-diamidino-2-phenylindole (DAPI); DAPI; Fluorescein (FITC); Rhodamine
name:
formaldehyde fixed tissue, tissue in paraffin embedment
name:
breast
name:
carcinogenesis
name:
nuclear chromatin
name:
fluorescence microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/13436.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13436.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13436.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_nc_sa
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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