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Title: Cell Image Library Dataset CIL:13389      
dateReleased:
04-29-2011
description:
This image is one of a group of 4 images that supports the conclusion that hRio2 kinase activity is required for cytoplasmic 18S-E pre-rRNA processing. This image shows the distribution of all 18S rRNA precursors (white), via fluorescence in situ hybridization (FISH) with a 5' ITS1 probe, upon depletion of a protein important in rRNA processing, hRio2, and subsequent attempted rescue by transfection with an EGFP-tagged kinase-dead version of hRio2, in Hela cells. Depletion of hRio2 leads to the accumulation of 18S-E pre-rRNA in the cytoplasm (white), and this is rescued only by WT hRio2 (a companion image in this group), but not by hRio2 protein that lacks kinase activity, seen here in green. Note that the 5' ITS1 probe shows the localization of all 18S rRNA precursors, but only 18S-E pre rRNA is detected in the cytoplasm. HeLaK cells were transfected with Rio2-d siRNA and then the rescue construct was transfected after 48 h of RNAi. Cells were fixed after 72 h of RNAi, followed by FISH analysis. In the set of four images in this group, FISH pictures were processed in parallel by enhancing levels, followed by setting gamma correction to 0.75.
privacy:
not applicable
aggregation:
instance of dataset
ID:
13389
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
Cy5; EGFP; GFP
name:
detergent permeabilized, formaldehyde fixed tissue
name:
maturation of SSU-rRNA
name:
processing of 20S pre-rRNA
name:
HeLa
name:
43S preribosome
name:
preribosome
name:
small subunit precursor
name:
confocal microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/13389.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13389.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13389.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_nc_sa
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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