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Title: Cell Image Library Dataset CIL:13376      
dateReleased:
04-18-2011
description:
Z-focal series through HeLa cell in mitosis illustrating MCAK localization in Bod1siRNA cells. Cells were transfected with control (CIL: 13377) or Bod1 (this image) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.
privacy:
not applicable
aggregation:
instance of dataset
ID:
13376
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
primary antibody plus labeled secondary antibody
name:
chemically fixed tissue
name:
Bod1 siRNA
name:
mitosis
name:
HeLaS3
name:
HeLa S3
name:
centromere
name:
chromosome
name:
centromeric region
name:
DNA
name:
HEC1
name:
MCAK
name:
fluorescence microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/13376.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13376.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13376.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_nc_sa
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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