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Title: Cell Image Library Dataset CIL:13124      
dateReleased:
04-20-2011
description:
A high resolution micrograph of the collecting canal of the contractile vacuole surrounded by smooth spongiome remnants and decorated tubules of the CVC. Microtubules bordering the flattened collecting canal are labeled with immunogold when thin frozen sections of the CVC are exposed by the indirect immunogold technique to show anti-b-tubulin. These microtubules have their origin at the cytosolic side of the CV pore. TEM taken on 2/25/94 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are outlined in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
privacy:
not applicable
aggregation:
instance of dataset
ID:
13124
refinement:
curated
availability:
available
types:
image
dimensions:
electron density
keywords:
gold; positive staining; stain with broad specificity; uranyl salt
name:
glutaraldehyde fixed tissue, microtome-sectioned tissue, monoclonal antibody, tissue in amorphous ice embedment, tissue in vitreous ice embedment
name:
cell by organism
name:
Ciliated Protist
name:
eukaryotic cell
name:
Eukaryotic Protist
name:
contractile vacuole organisation
name:
contractile vacuole organization
name:
contractile vacuolar membrane
name:
contractile vacuole
name:
contractile vacuole membrane
name:
cytoplasmic microtubule
name:
non-spindle-associated astral microtubule
name:
detection of electrons, film
accessURL: www.cellimagelibrary.org/images/download_jpeg/13124.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13124.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13124.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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