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Title: Cell Image Library Dataset CIL:13111      
dateReleased:
02-11-2011
description:
Cross section through a radial arm of the contractile vacuole complex. The collecting canal occupies the center of the arm whose membrane is supported by the radial microtubular ribbon which has now divided into at least six segments and surrounds the canal. Gaps between the segments are where the mass of membrane tubules of the smooth spongiome opens into the canal. Decorated tubules occurring in bundles form the peripheral layer of the radial arm. TEM taken on 5/2/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,000X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13105) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
privacy:
not applicable
aggregation:
instance of dataset
ID:
13111
refinement:
curated
availability:
available
types:
image
dimensions:
electron density
keywords:
lead salt; osmium tetroxide; positive staining; stain with broad specificity; uranyl salt
name:
glutaraldehyde fixed tissue, microtome-sectioned tissue, osmium tetroxide fixed tissue, tissue in epoxy resin embedment
name:
cell by organism
name:
Ciliated Protist
name:
eukaryotic cell
name:
Eukaryotic Protist
name:
contractile vacuole organisation
name:
contractile vacuole organization
name:
microtubule-based process
name:
water transport
name:
contractile vacuole
name:
contractile vacuole collecting tubule
name:
contractile vacuole radial arm
name:
microtubule associated complex
name:
detection of electrons, film
accessURL: www.cellimagelibrary.org/images/download_jpeg/13111.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13111.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13111.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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