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Title: Cell Image Library Dataset CIL:12977      
dateReleased:
02-09-2011
description:
The cortex of Paramecium consists of a plasma membrane and alveolar sacs, with their inner and outer alveolar membranes that limit the area in which endocytosis can easily occur to only the parasomal sacs. Parasomal sacs are cylindrical indentations of the plasma membrane that pass through the alveoli, not by penetrating through the sac but by passing through the septum. A short branch of the septum passes around the parasomal sac. In some areas the parasomal sacs extend almost perpendicularly from the basal bodies (not shown in this micrograph). There is one parasomal sac per surface depression in non-dividing cells. Rough ER, mitochondria, peroxisomes, trichocysts, and kinetodesmal fiber stack are seen in this image. The cell was incubated in hydrogen peroxide and diaminobenzidine to label the peroxisomes. TEM taken on 2/27/81 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12610) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
privacy:
not applicable
aggregation:
instance of dataset
ID:
12977
refinement:
curated
availability:
available
types:
image
dimensions:
electron density
keywords:
diaminobenzidine; lead salt; osmium tetroxide; positive staining; stain with broad specificity; uranyl salt
name:
glutaraldehyde fixed tissue, microtome-sectioned tissue, osmium tetroxide fixed tissue, tissue in epoxy resin embedment
name:
Ciliated Protist
name:
eukaryotic cell
name:
Eukaryotic Protist
name:
clathrin coating of vesicles
name:
cortical organization
name:
peroxisome localisation
name:
peroxisome localization
name:
plasma membrane organisation
name:
plasma membrane organization
name:
bacterial inner membrane
name:
cell cortex
name:
cell periphery
name:
clathrin coat of coated pit
name:
internal side of plasma membrane
name:
juxtamembrane
name:
peroxisome
name:
peroxisome vesicle
name:
plasma membrane
name:
trichocyst
name:
detection of electrons, film
accessURL: www.cellimagelibrary.org/images/download_jpeg/12977.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12977.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12977.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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