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Title: Cell Image Library Dataset CIL:12957      
dateReleased:
10-17-2011
description:
Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and GOLPH3 (a trans-Golgi associated protein that can also be associated with mitochondria; smaller gold particles) staining. Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.
privacy:
not applicable
aggregation:
instance of dataset
ID:
12957
refinement:
curated
availability:
available
types:
image
dimensions:
electron density
keywords:
gold; primary antibody plus labeled secondary antibody; uranyl salt
name:
cryostat-sectioned tissue, embedded tissue, formaldehyde fixed tissue, methylcellulose embedded
name:
glandular epithelial cell
name:
mammary alveolar cell
name:
milk secreting cell
name:
lactation
name:
lipid retention
name:
lipid storage
name:
secretion
name:
adiposome
name:
Golgi trans face
name:
lipid particle
name:
mitochondria
name:
mitochondrion
name:
SER
name:
smooth endoplasmic reticulum
name:
trans-Golgi network
name:
detection of electrons, illumination by electrons, transmission electron microscopy (TEM)
accessURL: www.cellimagelibrary.org/images/download_jpeg/12957.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12957.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12957.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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