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Title: Cell Image Library Dataset CIL:12980      
dateReleased:
03-15-2011
description:
High magnification of early endosomes in which the membranes are easily seen as a tripartite track. Gold, labeling antigen C6 is observed only in the body of the early endosome and not in the coated evagination or small coated vesicle lying nearby, which may be a newly released carrier vesicle or a coated evagination still attached to an early endosome. TEM taken on 5/14/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are outlined in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12642) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
privacy:
not applicable
aggregation:
instance of dataset
ID:
12980
refinement:
curated
availability:
available
types:
image
dimensions:
electron density
keywords:
gold; positive staining; stain with broad specificity; uranyl salt
name:
glutaraldehyde fixed tissue, microtome-sectioned tissue, monoclonal antibody, tissue in amorphous ice embedment, tissue in vitreous ice embedment
name:
cell by organism
name:
Ciliated Protist
name:
eukaryotic cell
name:
Eukaryotic Protist
name:
early endosome to late endosome transport
name:
endosome organisation
name:
endosome organization
name:
bacterial inner membrane
name:
early endosome
name:
gold labelled antigen
name:
plasma membrane
name:
detection of electrons, film
accessURL: www.cellimagelibrary.org/images/download_jpeg/12980.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12980.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12980.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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