Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Cell Image Library Dataset CIL:1311      
dateReleased:
02-18-2011
description:
High resolution image of the interphase micronucleus with only a small amount of b-tubulin in its matrix or on its nuclear envelope. Segments of microtubules are scattered in the cytosol. Immunogold labeled for anti-b-tubulin. TEM taken on 7/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a Nikon Coolscan 9000ED. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
privacy:
not applicable
aggregation:
instance of dataset
ID:
1311
refinement:
curated
availability:
available
types:
image
dimensions:
electron density
keywords:
gold; positive staining; stain with broad specificity; uranyl salt
name:
glutaraldehyde fixed tissue, microtome-sectioned tissue, monoclonal antibody, tissue in amorphous ice embedment, tissue in vitreous ice embedment
name:
cell by organism
name:
Ciliated Protist
name:
eukaryotic cell
name:
Eukaryotic Protist
name:
cytoplasm organisation
name:
cytoplasm organization
name:
micronuclear organization
name:
micronucleus organization
name:
cytoplasm
name:
cytoplasmic microtubule
name:
micronucleus
name:
non-spindle-associated astral microtubule
name:
detection of electrons, film
accessURL: www.cellimagelibrary.org/images/download_jpeg/1311.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/1311.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/1311.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.