Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Cell Image Library Dataset CIL:12810      
dateReleased:
02-05-2011
description:
This movie shows the "yolk flash". The apparently whole-embryo calcium pulse appears to initiate over a widespread area of blastoderm, possibly earliest at the animal pole, and then spreads first to the yolk cell membrane and then along the ventral midline heading rostrally. 30-sec integration window moving in 5-sec steps, elapsed real time of 340 sec played at 50x real time.. Fertilized zebrafish eggs were collected within 5 min of spawning, enzymatically dechorionated, and injected with approximately 0.9 nl of a 1% solution of recombinant f-aequorin in 100 mM KCl, 5 mM Mops, and 50 μM EDTA. During imaging the embryos were maintained at 28°C in 30% Danieau's medium containing penicillin (0.5 mg/ml), streptomycin (5,000 units/ml; Sigma), and 0.5% methylcellulose. Imaging was performed on a Photon Imaging Microscope (Science Wares, Falmouth, MA) that used a photon-counting spatial detector with a resistive anode output (Photek, St. Albens-on-Sea, U.K.). Digitized detector output in the form of a stream of time-labeled eight-bit x--y coordinates (256 × 256 pixels) was used to construct time-lapse imaging sequences. The imaging system software allowed the original photon data stream to be analyzed according to any chosen integration time, with the resulting image frames maintaining accurate photon quantitation up to 256 photons per pixel. These images are part of an image series within the Zebrafish--The Living Laboratory CD made available by Mark Cooper and described in Methods in Cell Biology Volume 77, 2004, Pages 439-457. Corresponds to supplemental video in PNAS January 5, 1999 vol. 96: 157-161 and quantification of color scale shown in Fig 1.
privacy:
not applicable
aggregation:
instance of dataset
ID:
12810
refinement:
curated
availability:
available
types:
image
dimensions:
bioluminescence
keywords:
f-aequorin
name:
live-cell imaging, living tissue, microinjection
name:
Ca2+ ion detection
name:
detection of calcium ion
name:
Embryo
name:
fluorescence microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/12810.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa, copyright
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12810.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa, copyright
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12810.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_nc_sa, copyright
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.