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Title: Cell Image Library Dataset CIL:12655      
dateReleased:
02-18-2011
description:
Human primary keratinocyte spreading on an asymmetric "arc"-shaped collagen I micropattern. The cells are stained for the focal adhesion protein vinculin (green, Alexa488), F-Actin (red, TRITC phalloidin) and the nucleus (blue, DAPI). The polymer brush micropatterning technique used is described in Gautrot et al. Biomaterials 2010, 31, 5030-5040. Images were acquired on a Leica DMI4000 using a 63X, NA: 1.25 and a Leica DFC 340 FX camera with 17 ms exposure for blue; 117 ms for green, and 159 ms for red.
privacy:
not applicable
aggregation:
instance of dataset
ID:
12655
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
4',6-diamidino-2-phenylindole (DAPI); Alexa Fluor 488; DAPI; phalloidin; primary antibody plus labeled secondary antibody; Tetramethyl rhodamine (TRITC)
name:
formaldehyde fixed tissue, permeabilized tissue
name:
keratinized cell of epidermis
name:
keratinocyte
name:
cell spreading during cell substrate adhesion
name:
substrate adhesion-dependent cell spreading
name:
actin cytoskeleton
name:
cell nucleus
name:
focal adhesion
name:
focal contact
name:
nucleus
name:
fluorescence microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/12655.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
copyright
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12655.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
copyright
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/12655.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
copyright
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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