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Title: Cell Image Library Dataset CIL:13458      
dateReleased:
03-30-2011
description:
Intracellular localization of GFP-Vps74 in S. cerevisiae pik1-83 temperature-conditional strain grown at permissive temperature of 26C (control for 37C temperature-shift). In pik1-83 mutant after a 30 minute incubation at the restrictive temperature of 37C, GFP-Vps74 loses punctate localization and is mostly in the cytosol and nucleus. The Golgi-localized PtdIns 4-kinase in yeast is composed of the Pik1 catalytic subunit and a myristoylated calcium-binding protein, Frq1. Vps74 is essential to maintain glycosyltransferases in the Golgi and results in this study reveal a previously unrecognized PtdIns4P-binding site in Vps74/GOLPH3 family proteins and link Pik1 signaling to retention of Golgi-resident proteins. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1A Vps74/pik1-83 26C panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 1A include CIL#s 13439, 13440, 13441, 13442, 13443, 13444, 13445, 13446, 13447, 13448, 13458, 13459.
privacy:
not applicable
aggregation:
instance of dataset
ID:
13458
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
EGFP; GFP
name:
live-cell imaging, living tissue
name:
cis-Golgi to rough endoplasmic reticulum transport
name:
enzyme transport
name:
inositol phospholipid-mediated signaling
name:
phosphatidylinositol-mediated signaling
name:
protein localisation in Golgi apparatus
name:
protein localization in Golgi apparatus
name:
protein transport
name:
retrograde vesicle-mediated transport
name:
Golgi to ER
name:
pik1-83
name:
cell nucleus
name:
cytosol
name:
extrinsic to membrane
name:
Golgi
name:
Golgi apparatus
name:
Golgi trans face
name:
nucleus
name:
peripheral membrane protein
name:
trans-Golgi network
name:
fluorescence microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/13458.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13458.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_nc_sa
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/13458.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_nc_sa
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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